Genetic Relationships among Aedes aegypti Collections in Venezuela as Determined by Mitochondrial DNA Variation and Nuclear Single Nucleotide Polymorphisms

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE - Tập 78 Số 3 - Trang 479-491 - 2008
Ludmel Urdaneta-Márquez1, Christopher F. Bosio, Flor Herrera, Yasmín Rubio-Palis, Michael L Salasek, Saúl Lozano‐Fuentes
1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA. [email protected]

Tóm tắt

A population genetic analysis of gene flow was conducted among 619 Aedes aegypti from nine collections distributed among six geographic regions of Venezuela. Genetic markers included a 387-basepair region of the mitochondrial NADH dehydrogenase 4 (ND4) gene and single nucleotide polymorphisms (SNPs) at 11 nuclear loci. Genotypes at SNP loci were identified using melting curve analysis. Six different ND4 haplotypes were detected and patterns of variation suggested that collections were isolated by distance. The variance in SNP allele frequencies was much less than the variance in haplotype frequencies and a pattern of isolation by distance was not detected. Aedes aegypti from eight collections were orally challenged with dengue 2 virus. Disseminated infection rates ranged from 77% to 95%. The percentage of mosquitoes exhibiting a midgut infection barrier ranged from 2% to 15%, and those exhibiting a midgut escape barrier ranged from 2% to 18%. Venezuelan Ae. aegypti appear to be susceptible to dengue virus infection.

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Tài liệu tham khảo

1994, 91, 2395

2000

2002, 33, 330

2002, 10, 100

2000

2002

1995, 29, 193

2005, 10, 597

2001, 53, 37

2001, 38, 623

2002, 67, 85

1979, 28, 1045

1985, 34, 1219

1991, 37, 14

1979, 34, 215

1983, 41, 241

1996, 76, 325

2002, 66, 213

2000, 62, 200

2006, 101, 625

2005, 100, 639

2002, 11, 1629

2003, 12, 631

2008

2005, 35, 771

2007, 16, 377

2003, 34, 1068

2002, 47, 593

1997

2003, 19, 2496

1990, 125, 873

1999, 9, 72

2005, 39, 885

1996, 21, 20

1992, 131, 479

1993, 47, 264

1962, 193, 855

1999, 15, 1068

1985, 70, 491

1986, 155, 365

2006, 37, 760

2002, 67, 85

2005, 42, 110

1985, 11, 41

1985, 66, 1369

2007, 16, 785

2005, 72, 434

1984, 38, 1358

2005, 42, 110

1965, 19, 395

1991, 58, 167

2001, 157, 295

2006, 312, 570

1984, 33, 690

1985, 34, 1219

1985, 34, 1225

Thông tin
Thông tin xuất bản

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

Tập 78 Số 3

479-491

Thông tin tác giả