Generation of an NANS homozygous knockout human induced pluripotent stem cell line by the insertion of GFP-P2A-Puro via CRISPR/Cas9 editing

Stem Cell Research - Tập 49 - Trang 102052 - 2020
Qian Bu1,2,3, Huaqin Zhang2, Qian Liu2, Yanping Dai2, Qian Wei2, Aiqin Xue2, Yan Huang1,2,3, Kai Zhong3, Yina Huang1, Hong Gao3, Xiaobo Cen2
1West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China
2National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China
3Department of Food Science and Technology, College of Biomass and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China

Tài liệu tham khảo

Hao, 2005, Cloning, expression, and characterization of sialic acid synthases, Biochem. Biophys. Res. Commun., 338, 1507, 10.1016/j.bbrc.2005.10.113 Schnaar, 2014, Sialic Acids in the Brain: Gangliosides and Polysialic Acid in Nervous System Development, Stability, Disease, and Regeneration, Physiol. Rev., 94, 461, 10.1152/physrev.00033.2013 Steyer, 2018, Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection, Stem Cell Rep., 10.1016/j.stemcr.2017.12.004 Van Karnebeek, 2016, NANS-mediated synthesis of sialic acid is required for brain and skeletal development, Nat Genet, 10.1038/ng.3578
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Stem Cell Research

Tập 49

102052

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