Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system

Stem Cell Research - Tập 71 - Trang 103136 - 2023
Meiling Jiang1, Chengcheng Tang1, Xian Luo1, Xiaoqing Zhou1, Min Chen1, Yue Chi1, Liangxue Lai1,2,3,4, Qingjian Zou1
1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, Wuyi University, Jiangmen, China
2China-New Zealand Joint Laboratory on Biomedicine and Health, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
3Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou 510530, China
4Sanya Institute of Swine Resource, Hainan Provincial Research Centre of Laboratory Animals, Sanya 572000, China

Tài liệu tham khảo

Soppert, 2020, Lipoproteins and lipids in cardiovascular disease: from mechanistic insights to therapeutic targeting, Adv. Drug Deliv. Rev., 159, 4, 10.1016/j.addr.2020.07.019 Sugita, 2021, Detection of mycoplasma contamination in transplanted retinal cells by rapid and sensitive polymerase chain reaction test, Int. J. Mol. Sci., 22, 12555, 10.3390/ijms222212555 Sun, 2022, Generation of induced pluripotent stem cells from peripheral blood mononuclear cells obtained from an adult with autosomal recessive polycystic kidney disease, Stem Cell Res., 61, 102772, 10.1016/j.scr.2022.102772 Zhao, 2022, Mog1 deficiency promotes cardiac contractile dysfunction and isoproterenol-induced arrhythmias associated with cardiac fibrosis and Cx43 remodeling, Biochim. Biophys. Acta, 1868 Zhou, 2016, Cardiac sodium channel regulator MOG1 regulates cardiac morphogenesis and rhythm, Sci. Rep., 6, 21538, 10.1038/srep21538