Generation and characterization of a human induced pluripotent stem (iPS) cell line derived from an acute myeloid leukemia patient evolving from primary myelofibrosis carrying the CALR 52 bp deletion and the ASXL1 p.R693X mutation

Chicaybam, 2017, An efficient electroporation protocol for the genetic modification of mammalian cells, Front. Bioeng. Biotechnol., 4, 99, 10.3389/fbioe.2016.00099 Ferrer-Marin, 2013, Leukemic transformation driven by an ASXL1 mutation after a JAK2V617F-positive primary myelofibrosis: clonal evolution and hierarchy revealed by next- generation sequencing, J. Hematol. Oncol., 6, 68, 10.1186/1756-8722-6-68 Klampfl, 2013, Somatic mutations of calreticulin in myelproliferative neoplasms, N. Engl. J. Med., 369, 2379, 10.1056/NEJMoa1311347 Lion, 2012, The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation, Leukemia, 26, 1821, 10.1038/leu.2012.66 Nangalia, 2013, Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2, N. Eng. J. Med., 2391, 10.1056/NEJMoa1312542 Vainchenker, 2017, Genetic basis and molecular pathophysiology of classical myeloproliferative neoplasms, Blood, 129, 667, 10.1182/blood-2016-10-695940