Gelatin-based anionic hydrogel as biocompatible substrate for human keratinocyte growth
Tóm tắt
Ionic hydrogels are biocompatible candidates for skin tissue engineering. Two hydrogels synthesized by crosslinking gelatin with polylysine (positively charged HG1) or polyglutamic acid (negatively charged HG2) were tested using spontaneously immortalized human keratinocytes (HaCaT). HaCaT cells displayed higher adhesion and proliferation onto HG2, forming a continuous and stratified epithelium after 7 days. Moreover HaCaT cells grown onto HG2 showed a decreased Epilysin and Filaggrin expression, while transglutaminase-1 expression was increased. Those data indicate that human keratinocyte can form a stratified epithelium onto HG2 that could therefore be an useful tool for skin tissue engineering.
Tài liệu tham khảo
Feliciani C, Gupta AK, Sauder DN. Crit Rev Oral Biol Med. 1996;7:300–18.
Hakvoort TE, et al. Virchows Arch. 1999;434:221–6.
Powell HM, McFarland KL, Bulter DL, Supp DM, Boyce ST. Tissue Eng Part A. 2010;16:1083–92.
Rheinwald JG, Green H. Cell. 1975;6:331–43.
Machens HG, Berger AC, Mailaender P. Cells Tissues Organs. 2000;167:88–94.
Liu J, Bian Z, Kuijpers-Jagtman AM, Von den Hoff JW. Orthod Craniofac Res. 2010;13:11–20.
Layman H, Spiga MG, Brooks T, Pham S, Webster KA, Andreopoulos FM. Biomaterials. 2007;28:2646–54.
Tabata Y, Ikada Y. Biomaterials. 1999;20:2169–75.
Tabata Y, et al. Biomaterials. 1998;19:807–15.
Lalan S, Pomerantseva I, Vacanti JP. World J Surg. 2001;25:1458–66.
Ehrenreich M, Ruszczak Z. Tissue Eng. 2006;12:2407–24.
Kopeček J. Biomaterials. 2007;28:5185–92.
Peters T Jr. Adv Protein Chem. 1985;37:161–245.
Collier TO, Jenney CR, DeFife KM, Anderson JM. Biomed Sci Instrum. 1997;33:178–83.
Nimeri G, Ohman L, Elwing H, Wettero J, Bengtsson T. Biomaterials. 2002;23:1785–95.
McFarland CD, De Filippis C, Jenkins M, Tunstell A, Rhodes NP, Williams DF, Steele JG. J Biomater Sci Polym Edn. 1998;9:1227–39.
Presland RB, Dale BA. Crit Rev Oral Biol Med. 2000;11:383–408.
McGrath JA, Uitto J. Trends Mol Med. 2008;14:20–7.
Lohi J, Wilson CL, Roby JD, Parks WC. J Biol Chem. 2001;276:10134–44.
Saarialho-Kere U, Kerkelä E, Jahkola T, Suomela S, Keski-Oja J, Lohi J. J Invest Dermatol. 2002;119:14–21.
Renò F, Sabbatini M, Stella M, Magliacani G, Cannas M. Wound Repair Regen. 2005;13:255–61.
Hitomi K. Eur J Dermatol. 2005;15:313–9.
Matsuki M, et al. Proc Natl Acad Sci USA. 1998;95:1044–9.
Otani Y, Tabata Y, Ikada Y. J Biomed Mater Res. 1996;31:158–66.
Schmidt JJ, Rowley J, Kong HJ. J Biomed Mater Res. 2008;87A:1113–22.