Gastric anti-ulcerative and anti-inflammatory activity of metyrosine in rats

Pharmacological Reports - Tập 62 - Trang 113-119 - 2010
Abdulmecit Albayrak1, Beyzagul Polat1, Elif Cadirci1, Ahmet Hacimuftuoglu1, Zekai Halici1, Mine Gulapoglu2, Fatih Albayrak3, Halis Suleyman1
1Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
2Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey
3Department of Gastroenterology, Faculty of Medicine, Ataturk University, Erzurum, Turkey

Tóm tắt

In this study, the anti-inflammatory and anti-ulcerative effects of metyrosine, a selective tyrosine hydroxylase enzyme inhibitor, were investigated in rats. For ulcer experiments, indomethacin-induced gastric ulcer tests and ethanol-induced gastric ulcer tests were used. For these experiments, rats were fasted for 24 h. Different doses of metyrosine and 25 mg/kg doses of ranitidine were administered to rats, followed by indomethacin at 25 mg/kg for the indomethacin-induced ulcer test, or 50% ethanol for the ethanol-induced test. Results have shown that at all of the doses used (50, 100 and 200 mg/kg), metyrosine had significant anti-ulcerative effects in both indomethacin and ethanol-induced ulcer tests. Metyrosine doses of 100 and 200 mg/kg (especially the 200 mg/kg dose) also inhibited carrageenan-induced paw inflammation even more effectively than indomethacin. In addition, to characterize the anti-inflammatory mechanism of metyrosine we investigated its effects on cyclooxygenase (COX) activity in inflammatory tissue (rat paw). The results showed that all doses of metyrosine significantly inhibited high COX-2 activity. The degree of COX-2 inhibition correlated with the increase in anti-inflammatory activity. In conclusion, we found that metyrosine has more anti-inflammatory effects than indomethacin and that these effects can be attributed to the selective inhibition of COX-2 enzymes by metyrosine. We also found that adrenalin levels are reduced upon metyrosine treatment, which may be the cause of the observed gastro-protective effects of this compound.

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