Fungi associated with the decline of Pinus halepensis in Spain

Springer Science and Business Media LLC - Tập 40 - Trang 1-11 - 2010
L. Botella1, O. Santamaría2, J. J. Diez1
1Departamento de Producción Vegetal y Recursos Forestales, Universidad de Valladolid, Palencia, Spain
2Departamento de Ingeniería del Medio Agronómico y Forestal, Universidad de Extremadura, Badajoz, Spain

Tóm tắt

Fungal species richness and composition within needles and twigs in 55 stands of Pinus halepensis, spread out over the whole Iberian Peninsula, were determined. The aim was to evaluate the relationships of fungal communities with local environmental variables, in order to analyze the potential causes of the current decline of this pine species in Spain. A total of 35 fungal taxa were isolated from 1980 moist chambers analysed (990 per vegetal tissue). A taxon within the Alternaria alternata complex was most frequent, followed by Leptostroma pinastri, Aspergillus niger, Diplodia pinea and Phomopsis sp. At the tree level, tissue was a significant response variable and a higher species richness was found in needles as compared to twigs. On the other hand, the multivariate analysis showed the environmental variables ‘age’, ‘shadow’, ‘elevation’, ‘mean temperature’, ‘illumination’ and ‘availability of water’ significantly influenced fungal species composition. In particular, ‘mean temperature’, was an important variable implicated in the general weakening of this thermophilic pine species, and appeared to be inversely correlated with the occurrence of several conifer pathogens such as Brunchorstia pinea, Cytospora sp., Diplodia pinea, Naemacyclus niveus, Pestalotiopsis stevensonii and Sclerophoma pythiophila. This study shows a possible combined effect of abiotic and biotic stresses in causing the general decline of Allepo pine in Spain.

Tài liệu tham khảo

Abelló MA (1998) Historia y Evolución de las Repoblaciones Forestales en España. Universidad Complutense de Madrid, Madrid, p 749 Anonymous (1989) SAS/STAT user´s guide, vol 1–2, 4th edn. SAS Institute, Cary Arnold AE (2007) Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biol Rev 21:51–66 Arnold AE, Herre EA (2003) Canopy cover and leaf age affect colonization by tropical fungal endophytes: ecological patterns and process in Theobroma cacao (Malvaceae). Mycologia 95:388–398 Bachi PR, Peterson JL (1985) Enhancement of Sphaeropsis sapinea stem invasion of pines by water deficits. Plant Dis 69:798–799 Bahnweg G, Heller W, Stich S, Knappe C, Betz C, Heerdt C, Kehr RD, Ernst D, Langebartels C, Nunn AJ, Rothenburger J, Schubert R, Wallis P, Müller-Starck G, Werner H, Matyssek R, Sandermann H Jr (2005) Beech leaf colonization by the endophyte Apiognomia errabunda. Plant Biol 7:659–669 Brandt JP, Cerezke HF, Mallett KI, Volney WJA, Weber JD (2003) Factors affecting trembling aspen (Populus tremuloides Michx.) health in the boreal forest of Alberta, Saskatchewan, and Manitoba, Canada. For Ecol Manag 178:287–300 Brener WD, Setliff EC, Norgren RL (1974) Sclerophoma pythiophila associated with a tip dieback of Juniper in Wisconsin. Plant Dis Rep 58:653–657 Bussoti F, Ferreti M (1998) Air pollution, forest condition and forest decline in Southern Europe: an overview. Environ Pollut 101:49–65 Calamassi R, Paoletti E, Strati S (2001) Frost hardening and resistance in three Aleppo pine (Pinus halepensis Mill.) provenances. Isr J Plant Sci 49:179–186 Climent J, Costa e Silva F, Chambel MR, Pardos M, Almeida MH (2009) Freezing injury in primary and secondary needles of Mediterranean pine species of contrasting ecological niches. Ann For Sci 66:407 Collado J, Platas G, Peláez F (1996) Fungal endophytes in leaves, twigs and bark of Quercus ilex from central Spain. Nova Hedwigia 63:347–360 Danti R, Sieber TN, Sanguineti G (2002) Endophytic mycobiota in bark of European beech (Fagus sylvatica) in the Apennines. Mycol Res 106:1343–1348 De Wet J, Burgess T, Slippers B, Preisig O, Wingfield BD, Winfield MJ (2003) Multiple gene genealogies and microsatellite markers reflect relationships between morphotypes of Sphaeropsis sapinea and distinguish a new species of Diplodia. Mycol Res 107:557–566 Desprez-Loustau ML, MarÇais B, Nageleisen LM, Piou D, Vannini A (2006) Interactive effects of drought and pathogens in forest trees. Ann For Sci 63:597–612 Farr D, Bills G, Chamuris G, Rossman A (1989) Fungi on Plants and plant products in the United States, St. Paul, MN Ganley RJ, Newcombe G (2006) Fungal endophytes in seeds and needles of Pinus monticola. Mycol Res 110:318–327 Gil, L., Díaz-Fernández, P.M., Jiménez, M.P., Roldán, M., Alía, R., Agúndez, D., De Miguel, J., Martín, S. and De Tuerto, M. (1996). Las regiones de Procedencia del Pinus halepensis Mill., Madrid Gimeno BS, Peñuelas L, Porcuna JL, Reinert RA (1995) Biomonitoring ozone phytotoxicity in eastern Spain. Water Air Soil Pollut 85:1521–1526 Giordano L, Gonthier P, Varese GC, Miserere L, Nicolotti G (2009) Mycobiota inhabiting sapwood of healthy and declining Scots pine (Pinus sylvestris L.) trees in the Alps. Fungal Divers 38:xx–xx Goidanich G (1990) Manuale di patologia vegetale. Edizioni Agricole della Calderini, Bologna, p 1283 Gonthier P, Gennaro M, Nicolotti G (2006) Effects of water stress on endophytic mycota of Quercus robur. Fungal Divers 21:69–80 Guo LD, Xu L, Zheng WH, Hyde KD (2004) Genetic variation of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis as determined by random amplified microsatellites (RAMS). Fungal Divers 16:53–65 Hanlin RT (1998) Illustrated genera of ascomycetes, vol I and II. The American Phytopathology Society, St. Paul Hoff JA, Klopfenstein NB, McDonald GI, Tonn JR, Kim MS, Zambino PJ, Hessburg PF, Rogers JD, Peever TL, Carris LM (2004) Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa). Forest Pathol 34:255–271 Hu HL, Jeewon R, Zhou DQ, Zhou TX, Hyde HD (2007) Phylogenetic diversity of endophytic Pestalotiopsis species in Pinus armandii and Ribes spp.: evidence from rDNA and β-tubulin gene phylogenies. Fungal Divers 24:1–22 Huang WY, Cai YZ, Hyde KD, Corke H, Sun M (2008) Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Divers 33:61–75 Johnson JW, Gleason ML, Parker SK, Provin EB, Iles JK (1997) Duration of water stress affects development of Sphaeropsis canker on Scots pine. J Arboric 23:73–76 Jurc M, Jurc D, Gogala N, Simoncic P (1996) Air pollution and fungal endophytes in needles of Austrian pine. Phyton-Annales Rei Botanicae 36:111–114 Kiffer E, Morelet M (1999) The deuteromycetes. Mitosporic fungi. Classification and generic keys. Science Publishers Inc., Enfield La Porta N, Capretti P, Thomsen IM, Kasanen R, Hietala AM, Von Weissenberg K (2008) Forest pathogens with higher damage potential due to climate change in Europe. Can J Plant Pathol 30:177–195 Lanier L, Joly P, Bonodoux P, Bellemere A (1978) Mycologie et pathologie forestieres, vol 1. Masson, Paris Le Houerou HN (1992) Vegetation and land use in the Mediterranean basin by the year 2050: a prospective study. Edward Arnold, London Legendre P, Legendre L (1998) Numerical ecology. Elsevier, Amsterdam Lehtijärvi A, Barklund P (2000) Seasonal patterns of colonization of Norway spruce needles by Lophodermium piceae. Forest Pathol 30:187–193 Magan N, Kirkwood IA, Mcleod AR, Smith MK (1995) Effect of open-air fumigation with sulfur-dioxide and ozone on Phyllosphere and endophytic fungi of conifer needles. Plant Cell Environ 18:291–302 Martín P, Pajares JA, Nanos N, Diez JJ (2004) Site and seasonal influences on the fungal community on leaves and stems of Pinus and Quercus seedlings in forest nurseries. Sydowia 56:23–47 Müller MM, Hallaksela AM (1998) Diversity of Norway spruce needle endophytes in various mixed and pure Norway spruce stands. Mycol Res 102:1183–1189 Müller MM, Hallaksela AM (2000) Fungal diversity in Norway spruce: a case study. Mycol Res 104:1139–1145 Muñoz López C (1997) Presencia en España del hongo Sirococcus conigenus Cannon & Minter responsable de la muerte de brotes en Pinus halepensis Mill. XIV Reunión del Grupo de Trabajo Fitosanitario de Forestales, Parques y Jardines, pp 110–111 Ninyerola M, Pons X, Roure JM (2005) Altas Climático Digital de la Península Ibérica. In: Metodología y aplicaciones en bioclimatología y geobotánica (ed) Bellaterra. Universidad Autónoma de Barcelona Paoletti E, Danti R, Strati S (2001) Pre- and post- inoculation water stress affects Spaheropsis sapinea canker length in Pinus halepensis seedlings. Forest Pathol 31:209–218 Phillips DH, Burdekin DA (1992) Diseases of forest and ornamental trees. Macmillian, London Promputtha I, Lumyong S, Dhanasekaran V, McKenzie EHC, Hyde KD, Jeewon R (2007) A phylogenetic evaluation of whether endophytes become saprotrophs at host senescence. Microb Ecol 53:579–590 Punithalingam E, Waterson J (eds) (1970) Diplodia pinea. Pathogenic fungi and bacterial, vol 2. Commonwealth Mycological Institute, Kew Ranta H, Saloniemi I (2005) Distribution of fungal foliage and shoot pathogens in a natural Scots pine population in relation to environmental variables. Can J For Res-Revue Canadienne De Recherche Forestiere 35:503–510 Rishbeth J, Meredith DL (1957) Surface micoflora of pine needles. Nature 179:682–683 Saikkonen K (2007) Forest structure and fungal endophytes. Fungal Biol Rev 21:67–74 Santamaria O, Diez JJ (2005) Fungi in leaves, twigs and stem bark of Populus tremula from northern Spain. Forest Pathol 35:95–104 Santamaria O, Pajares JA, Diez JJ (2003) First report of Gremmeniella abietina on Pinus halepensis in Spain. Plant Pathol 52:425–425 Santamaria O, Tejerina L, Pajares JA, Diez JJ (2007) Effects of associated fungi Sclerophoma pythiophila and Cenangium ferruginosum on Gremmeniella abietina dieback in Spain. Forest Pathol 37:121–128 Santamaria, O., Botella, L. and Diez, J.J. (2008). Gremmeniella abietina in North-western Spain: Distribution and associated mycoflora. Acta Silvatica and Lignaria Hungarica, Spec. Edition: 137–145 Sieber B (2007) Endophytic fungi of forest trees: are they mutualists? Fungal Biol Rev 21:75–89 Siebercanavesi F, Petrini O, Sieber TN (1991) Endophytic Leptostroma species on Picea abies, Abies alba, and Abies balsamea—a Cultural, Biochemical, and Numerical Study. Mycologia 83:89–96 SPCAN (1998) Distribución de Daños Soflamado Observados en Pinus halepensis Speer JH, Grissino-Mayer HD, Orvis KH, Greenberg CH (2009) Climate response of five oak species in the eastern deciduous forest of the southern Appalachian Mountains, USA. Can J Bot-Revue Canadienne De Botanique 39:507–518 Stanosz GR, Blodgett JT, Smith DR, Kruger EL (2001) Water stress and Sphaeropsis sapinea as a latent pathogen of red pine seedlings. New Phytol 149:531–538 Stanosz GR, Smith DR, Leisso R (2007) Diplodia shoot blight and asymptomatic persistence of Diplodia pinea on or in stems of jack pine nursery seedlings. Forest Pathol 37:145–154 Stirling GR, Smith LJ, Licastro KA, Eden LM (1998) Control of root-knot nematode with formulations of the nematode-trapping fungus Arthrobotrys dactyloides. Biol Control 11:224–230 Sutton BC (1980) The coelomycetes. Fungi Imperfecti with Pycnidia Acervuli and Stromata. Commonwealth Mycological Institute, Kew Sutton BC, Waterston JM (1970) Colletotrichum musae. CMI Description of pathogenic fungi and bacteria. CMI. No. 222 Ter Braak CJF, Smilauer P (2002) CANOCO Reference Manual and CANODRAW for User´s Guide version 4.5., Ithaca, NY Thomas FM, Blank R, Hartmann G (2002) Abiotic and biotic factors and their interactions as causes of oak decline in Central Europe. Forest Pathol 32:277–307 Thomsen IM (2009) Precipitation and temperature as factors in Gremmeniella abietina epidemics. Forest Pathol 39:56–72 Wang Y, Guo LD, Hyde KD (2005) Taxonomic placement of sterile morphotypes of endophytic fungi from Pinus tabulaeformis (Pinaceae) in northeast China based on rDNA sequences. Fungal Divers 20:235–260 Zamora P, Martinez-Ruiz C, Diez JJ (2008) Fungi in needles and twigs of pine plantations from Northern Spain. Fungal Divers 30:171–184