Graphene oxide chức năng hóa quy mô nano cho việc vận chuyển thuốc cisplatin hiệu quả cao

Springer Science and Business Media LLC - Tập 16 - Trang 1-14 - 2014
Lingyang Tian1,2, Xibo Pei1,2, Yongxiang Zeng1,2, Rui He1,2, Zhongjie Li1,2, Jian Wang1,2, Qianbing Wan1, Xiaoyu Li2
1Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China
2State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China

Tóm tắt

Oxit graphene đã thu hút được sự quan tâm đặc biệt trong việc vận chuyển thuốc. Cấu trúc sp2-aromatic và các nhóm chứa oxy phong phú của oxit graphene quy mô nano (NGO) thường được sử dụng để tải các loại thuốc chống ung thư, dẫn đến hiệu suất tải cao. Nghiên cứu này sử dụng oxit graphene chức năng hóa polyethylene glycol (PEG, PL-PEG(2000)-NH2) như một phương tiện vận chuyển thuốc cho cis-diamminedichloroplatinum (II) (cisplatin, CDDP). Phản ứng cộng hóa trị giữa nguyên tử platinum (II) và nhóm carboxylic đã được sử dụng để gắn CDDP lên NGO-PEG. NGO, NGO-PEG và các nanohybride NGO-PEG/CDDP đã được đặc trưng hóa bằng hình ảnh kính hiển vi lực nguyên tử, hình ảnh kính hiển vi điện tử truyền qua, phổ hồng ngoại biến đổi Fourier và quang phổ Raman. Từ hình ảnh AFM, độ dày trung bình của các nanohybride trong khoảng từ 3.4 đến 7.0 nm và đường kính trung bình của phiến trong khoảng từ 21.7 đến 30.5 nm. NGO-PEG thể hiện hiệu suất tải CDDP cải thiện lên đến 0.58 mg mg−1. Các nanohybride NGO-PEG/CDDP đã giải phóng CDDP theo một mô hình duy trì trong 72 giờ và thể hiện độ độc tế bào đáng kể đối với tế bào ung thư vú người MCF-7 và tế bào adenosqumous carcinoma miệng CAL-27 qua các thử nghiệm in vitro. Phương tiện vận chuyển thuốc NGO-PEG được quan sát là không độc. Tình trạng ức chế sự phát triển tế bào và biến dạng hình thái do NGO-PEG/CDDP gây ra đã được minh họa thêm bằng hình ảnh huỳnh quang.

Từ khóa

#oxit graphene #vận chuyển thuốc #cisplatin #hiệu quả tải #ung thư vú #độc tính tế bào

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