Fumonisin B1 and ochratoxin A nephrotoxicity in Japanese quail: an ultrastructural assessment

Comparative Clinical Pathology - Tập 22 - Trang 835-843 - 2012
Manzoor Ahmad Khan1, R. K. Asrani1, Asif Iqbal2, R. D. Patil1, G. E. Rottinghaus3, D. R. Ledoux3
1Department of Veterinary Pathology, COVAS, CSK HPKV, Palampur, India
2Division of Veterinary Epidemiology and Preventive Medicine, SKUAST-JAMMU, Jammu, India
3Fusarium/Poultry Research Laboratory, University of Missouri, Columbia, USA

Tóm tắt

In the present study, fumonisin B1 (200 ppm) and ochratoxin A (2 ppm) were fed alone and in combination to day old Japanese quail chicks for 21 days to evaluate renal ultrastructural changes. The severity and intensity of renal ultrastructural changes varied with the type of treatment, and predominant and consistent lesions were found in the proximal convoluted tubule (PCT) lining cells. Distortion and dilatation of cisternae of endoplasmic reticulum leading to the formation of vesicular and tubular structures was a consistent finding noticed in the fumonisin B1-fed group. Ochratoxin A treatment revealed characteristic changes in the epithelial brush border of PCTs with blunting and variable loss of microvilli. Reduced number of mitochondria with mitochondrial pleomorphism (round, oval, curved, elongated, dumbbell, banana, pear, and tennis racket) was almost a consistent finding noticed in the ochratoxin A treatment group. Ultrastructural changes in the combination fed group were almost similar to those noticed in the individual treatment groups but were of a higher intensity. Or in other words, we can say that on simultaneous exposure, fumonisin B1 potentiated the toxic effects of OTA on renal ultrastructure.

Tài liệu tham khảo

Gelderblom WCA, Marasas WFO, Theil PG, Vleggar R, Cawood ME (1992) Fumonisin: isolation, chemical characterization and biological effects. Mycopathologia 117:11–16 Ross PF, Rice LG, Plattner RD, Osweiler GD, Wilson TM (1991) Concentrations of fumonisin B1 in feeds associated with animal health problems. Mycopathologia 114:129–135 Marasas WFO, Kellerman TS, Gelderblom WCA, Coetzer JAW, Theil PG (1988) Leukoencephalomalacia in a horse induced by fumonisin B1 isolated from Fusarium moniliforme. Onderstepoort J Vet Res 55:197–203 Harrison LR, Clvin BM, Greene TJ, Cole NLE Jr (1990) Pulmonary edema and hydrothorax in swine produced by FB1, a toxic metabolite of Fusarium moniliforme. J Vet Diagn Invest 2:217–221 Norred WP (1993) Fumonisins–mycotoxins produced by Fusarium moniliforme. J Toxicol Env Heal 38:309–328 Rheedar JP, Marasas WFO, Thiel PG, Sydenham EW, Shephard GS (1992) Fusarium moniliforme and fumonisins in corn in relation to human esophageal cancer in Transkei. Phytopathologia 82:353–357 Yoshizawa T, Yamashita A, Luo Y (1994) Fumonisin occurrence in corn from high and low risk areas for human esophageal cancer in China. Appl Env Microbiol 60:1626–1629 Bermudez AJ, Ledoux DR, Rottinghaus GE (1995) Effects of Fusarium moniliforme culture material containing known levels of FB1 in ducklings. Avian Dis 39:879–886 Ledoux DR, Brown TP, Weibking TS, Rottinghaus GE (1992) Fumonisin toxicity in broiler chicks. J Vet Diagn Invest 4:330–333 Weibking TS, Ledoux DR, Bermudez AJ, Turk JR, Rottinghaus GE (1995) Effects on turkey poults of feeding Fusarium moniliforme M-1325 culture material grown under different environmental conditions. Avian Dis 39:32–38 Weibking TS, Ledoux DR, Bermudez AJ, Turk JR, Rottinghaus GE, Wang E, Merrill AH Jr (1993) Effects of feeding Fusarium moniliforme culture material containing known levels of fumonisin B1 on the young broiler chicks. Poult Sci 72:456–466 Brown TP, Rottinghaus GE, Williams ME (1992) Fumonisin mycotoxicosis in broilers: performance and pathology. Avian Dis 36:450–454 Engelhardt JA, Carlton WW, Tuite JF (1989) Toxicity of Fusarium moniliforme var. subglutinans for chicks, ducklings and turkey poults. Avian Dis 33:357–360 Espada Y, Gopegui RR, Cuadradas C, Cabanes FJ (1997) Fumonisin mycotoxicosis in broilers: plasma protein and coagulation modifications. Avian Dis 41:73–79 Javed T, Bennet GA, Richard JL, Dombrink-Kruztman MA, Cote LM, Buck WB (1993) Mortality in broiler chicks on feed amended with Fusarium proliferatum culture material or with purified fumonisin B1 and moniliformin. Mycopathologia 123:171–184 Jeschke N, Nelson PE, Marasas WFO (1987) Toxicity to ducklings of Fusarium moniliforme isolated from corn intended for use in poultry feed. Poult Sci 66:1619–1623 Marijanovic DR, Holt P, Norred WP, Bacon CW, Voss KA, Stancel PC (1991) Immunosuppressive effects of Fusarium moniliforme corn cultures in chicken. Poult Sci 70:1895–1901 Anilkumar P, Satyanarayana ML, Vijaysarathi SK, Sreeniwasgowda RN, Rao S (2003) Pathology of lymphoid organs in aflatoxicosis and ochratoxicosis and mmunomodulatory effect of vitamin E and selenium in boiler chicken. Indian J Vet Path 27:102–106 Dwivedi P, Burns RB (1984a) Pathology of ochratoxin A in young broiler chicks. Res Vet Sci 36:92–103 Kumar A, Jindal N, Shukla CL, Yash P, Ledoux DR, Rottinghaus GE (2003) Effect of ochratoxin A on Escherichia coli-challenged broiler chicks. Avian Dis 47:415–424 Thaxton JP, Tung HT, Hamilton PB (1974) Immunosuppression in chicken by aflatoxin. Poult Sci 53:721–725 Samson RA (1992) Mycotoxins: a mycologist’s perspective. J Med Vet Mycol Supp 30:9–18 Peckham-John C, Ben-Doupnik JR, Oscar H, Jones JR (1971) Acute toxicity of ochratoxin A and B in chicks. Appl Microbiol 21:492–494 Stoev SD, Anguelov G, Ivanov I, Pavlov D (2000) Influence of ochratoxin A and an extract of antichoke on the vaccinal immunity and health in broiler chicks. Exp Toxicol Pathol 52:43–55 Stove SD, Koynarsky V, Mantle PG (2002) Clinicomorphological studies in chicks fed ochratoxin A while simultaneously developing coccidiosis. Vet Res Commun 26:189–204 Deshmukh S, Asrani RK, Ledoux DR, Jindal N, Bermudez AJ, Rottinghaus GE, Sharma M, Singh SP (2005) Individual and combined effects of Fusarium moniliforme culture material, containing known levels of fumonisin B1, and Salmonella gallinarum infection on liver of Japanese quail. Avian Dis 49:592–600 Solcan C, Coman I, Solcan G, Miron L, Oprean OZ (2008) Histological and ultrastructural lesions of the kidneys in experimental ochratoxicosis of broiler chickens. Bulletin of University of Agricultural sciences and Veterinary Medicine Cluj-Napoca-Veterinary Medicine 65:91–95 Dwivedi P, Burns RB (1984b) Effect of ochratoxin A on immunoglobulins in broiler chicks. Res Vet Sci 36:117–121 Creppy EE, Stormer FC, Kern D, Roschenthaler R, Dirheimer G (1983) Effects of ochratoxin A metabolites on yeast phenylalanyl-tRNA synthetase and on the growth and “in vivo” protein synthesis of hepatoma cells. Chem Biol Interact 47:239–247 Meisner N, Chan S (1974) Ochratoxin A, an inhibitor of mitochondrial transport system. J Biol Chem 13:2795–2800 Elling F, Nelsen JP, Lillehoj EB, Thomassen MS, Stormer FC (1985) Ochratoxin A induced porcine nephropathy: enzyme and ultrastructural changes after short term exposure. Toxicon 23:247–254 Stormer FC (1992) Ochratoxin A—a mycotoxin of concern. In: Bhatnagar D et al (eds) Handbook of applied mycology, Vol 5, Mycotoxins in ecological systems. Marcel Dekker, New York, pp 97–113 Hoehler D, Marquardt RR, Mclintosh AR, Hatch GM (1997) Induction of free radicals in hepatocyte, mitochondria and microsomes of rat by ochratoxin-A and its analogues. Biochem Biophys Acta 1357:225–233 Petrik KJ, Zanic-Grubisic T, Barisick, Pepeljnjak S, Radic B (2003) Apoptosis and oxidative stress induced by ochratoxin A in rat kidney. Arch Toxicol 77:685–693 Maxwell MH, Burns RB, Dwivedi P (1987) Ultrastructural study of ochratoxicosis in quail (Coturnix coturnix japonica). Res Vet Sci 42:228–231 Kumar M, Dwivedi P, Sharma AK, Singh ND, Patil RD (2007) Ochratoxin A and citrinin nephrotoxicity in New Zealand white rabbits: an ultrastructural assessment. Mycopathologia 163:21–30