Fructose-bisphosphatase-deficient mutants of mucoidPseudomonas aeruginosa
Tóm tắt
Fructose-bisphosphatase-deficient mutants of mucoidPseudomonas aeruginosa were isolated by ethyl methanesulfonate mutagenesis using gluconate as the nonpermissive substrate, and all the sixty isolates possessed 10–30% of the parental enzyme activity. The mutants had low levels of fructose-bisphosphate aldolase activity and could not normally synthesize alginate from any substrate except onPseudomonas isolation agar plates. The results suggest the essentiality of fructose bisphosphatase activity for the growth or survival ofP. aeruginosa and a probable linkage of genes controlling this enzyme with those of fructose bisphosphate aldolase and alginate biosynthesis.
Tài liệu tham khảo
Banerjee P.C., Vanags R.I., Chakrabarty A.M., Maitra P.K.: Alginic acid synthesis inPseudomonas aeruginosa mutants defective in carbohydrate metabolism.J. Bacteriol. 155, 238 (1983).
Banerjee P.C., Vanags R.I., Chakrabarty A.M., Maitra P.K.: Fructose 1,6-bisphosphate aldolase activity is essential for synthesis of alginate from glucose byPseudomonas aeruginosa.J. Bacteriol. 161, 458 (1985).
Banerjee P.C., Darzins A., Maitra P.K.: Gluconeogenic mutations inPseudomonas aeruginosa: Genetic linkage between fructose bisphosphate aldolase and phosphoglycerate kinase.J. Gen. Microbiol. 133, 1099 (1987).
Brammer W.J., Clarke P.H.: Induction and repression ofPseudomonas aeruginosa amidase.J. Gen. Microbiol. 37, 307 (1964).
Darzins A., Chakrabarty A.M.: Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate ofPseudomonas aeruginosa.J. Bacteriol. 159, 9 (1984).
Darzins A., Wang S.K., Vanags R.I., Chakrabarty A.M.: Clustering of mutations affecting alginic acid biosynthesis in mucoidPseudomonas aeruginosa.J. Bacteriol. 164, 516 (1985).
Fraenkel D.G., Horecker B.L.: Fructose 1,6-diphosphatase and acid hexose phosphatase ofEscherichia coli.J. Bacteriol. 90, 837 (1965).
Fujita Y., Freese E.: Isolation and properties of aBacillus subtilis mutant unable to produce fructose bisphosphatase.J. Bacteriol. 145, 760 (1981).
Hochster R.M., Katznelson H.: On the mechanism of glucose-6-phosphate oxidation in cell-free extracts ofXanthomonas phaseoli.Can. J. Biochem. Physiol. 36, 669 (1958).
Lessie T.G., Phibbs P.V. Jr.: Alternative pathways of carbohydrate utilisation in pseudomonads.Ann. Rev. Microbiol. 38, 359 (1984).
Ohman D.E., Chakrabarty A.M.: Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in aPseudomonas aeruginosa cystic fibrosis isolate.Infect. Immun. 33, 142 (1981).
Wood H., O'Brien W.E., Michaels G.: Properties of carboxytransphosphorylase, pyruvate phosphate dikinase, pyrophosphate-phosphofructokinase and pyrophosphate-acetate kinase and their roles in the metabolism of inorganic pyrophosphate.Adv. Enzymol. 45, 85 (1977).