Freezing curve based monitoring to quickly evaluate the viability of the biological materials subject to freezing and rewarming

Jing Liu1, Yi-Xin Zhou1
1Technical Institute of Physics and Chemistry, Chinese Academy and Sciences, Beijing, China

Tóm tắt

A new technique based on freezing curve monitoring to quickly evaluate the viability of the biological materials subject to freezing or rewarming was established. A practical integrated device was fabricated which is simple in structure and cheap in price. Preliminary freezing experiments on the fresh fish blood demonstrated that minor changes in a biological material due to freezing or warming injury may result in significant deviation in its freezing curve compared with that of the intact biomaterials. Three thermal indexes to quantify the damage degree of the biomaterials were pointed out. This method also opens many opportunities for the evaluation of biological material defect in diverse life science fields.

Từ khóa

#Monitoring #Biological materials #Cells (biology) #Cooling #Biochemical analysis #Magnetic analysis #Blood #Temperature #Fungi #Biological systems

Tài liệu tham khảo

liu, 1993, Cryomedicine (in Chinese), 1 baust, 1999, Cryopreservation of an engineered skin equivalent: the apoptosis paradigm, Advances in Heat and Mass Transfer in Biotechnology, 44, 71 10.1115/1.2895423 10.1126/science.168.3934.939 10.1080/08916159708946531 10.1021/bp00012a011 10.1016/0140-7007(91)90003-Y