Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion
Tóm tắt
Interspecies fusants are formed between Agaricus bisporus and Agaricus bitorquis by protoplast fusion technique. Protoplasts were isolated and regenerated by using Novozyme 234 lytic enzyme. Twenty slow growing isolates were separated from the protoplast regenerated colonies, which were assumed as homokaryons (putative homokaryons). These twenty isolates were subjected to growth rate, colony morphology and spawn run studies for screening of true homokaryons. Antifungal markers were developed for selection of fusants.
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Tài liệu tham khảo
Chen R, Chen L & Song S (2003) Identification of two thermotolerance-related genes in Agaricus bisporus. Food Technol Biotechnol 41:339–344
Sanchez C (2004) Modern aspects of mushroom culture technology. Appl Microbiol Biotechnol 64:756–762
Sonnenberg ASM (2000) Genetics and breeding of Agaricus bisporus. In: Science and Cultivation of Edible Fungi, (Griensven V eds). Balkema, Rotterdam, pp 25–39
Eichlerova I & Homolka L (1999) Preparation and crossing of basidiospore-derived monokaryons a useful tool for obtaining laccase and other ligninolytic enzyme higher producing dikaryotic strains of Pleurotus ostreatus. Antonie Leeuwenhoek 75:321–327
Dhitaphichit P & Pornsuriya C (2005) Protoplast fusion between Pleurotus ostreatus and P. djamor. Songklanakarin J Sci Technol 27:975–982
Peberdy JF (1987) Developments in protoplast fusion in fungi. Microbiol Sci 4:108–114
Crowhurst RN, Rees-George J, Rikkerink EHA & Templeton MD (1992) High efficiency transformation of Fusarium solani f. sp. cucurbitae race 2 (mating population V). Curr Genet 21:463–469
Sonnenberg ASM, Wessels JG & Van Griensven LJ (1988) An efficient protoplasting/regeneration system for Agaricus bisporus and Agaricus bitorquis. Curr Microbiol 17:285–291
Castle AJ, Horgen PA & Anderson JB (1988) Crosses among homokaryons from commercial and wild-collected strains of mushroom Agaricus brunnescens (=A. bisporus). Appl Environ Microbiol 54:1643–1648
Khush RS, Becker E & Wach M (1992) DNA amplification polymorphisms of the cultivated mushroom Agaricus bisporus. Appl Environ Microbiol 58:2971–2977
Jin JK & Xu HL (1990) Comparative study on fluffy and appressed types of Agaricus bisporus (L.) Imbach. Zhongguo-Shiyongjun. Edible-Fungi-of-China 9:12–13
Horgen PA (1992) The application of biotechnology to button mushroom, Agaricus bisporus. In: Applied Molecular Genetics of Filamentous Fungi, (Kinghorn JR & Turner G eds). Champman & Hall, Cambridge, UK, pp 191–200
Sobral JK, Lima EAA, De Araujo JM & Azevedo JL (2004) Genetic variability in regenerated Metarhizium flavoviride protoplasts. Braz Arch Biol Technol 47:1–8
Sandhu SS & Venkateswerlu G (1997) A rapid method for isolation of stable niaD and crnA mutants of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Fungal Genet Newslett 44:50–51
Dahlberg KR, US Patent, 4996390, (Campbell Soup Company), 26 Feb 1991. http://www.freepatentsonline.com/4996390.html