Formation and function of hepatitis C virus replication complexes require residues in the carboxy-terminal domain of NS4B protein

Ali, 2002, Cell-free replication of the hepatitis C virus subgenomic replicon, J. Virol., 76, 12001, 10.1128/JVI.76.23.12001-12007.2002 Alter, 1997, Epidemiology of hepatitis C, Hepatology, 26, 62S, 10.1002/hep.510260711 Berger, 2009, Roles for endocytic trafficking and phosphatidylinositol 4-kinase III alpha in hepatitis C virus replication, Proc. Natl. Acad. Sci. U. S. A., 106, 7577, 10.1073/pnas.0902693106 Blight, 2002, Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication, J. Virol., 76, 13001, 10.1128/JVI.76.24.13001-13014.2002 Egger, 2002, Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex, J. Virol., 76, 5974, 10.1128/JVI.76.12.5974-5984.2002 Einav, 2004, A nucleotide binding motif in hepatitis C virus (HCV) NS4B mediates HCV RNA replication, J. Virol., 78, 11288, 10.1128/JVI.78.20.11288-11295.2004 Einav, 2008, Discovery of a hepatitis C target and its pharmacological inhibitors by microfluidic affinity analysis, Nat. Biotechnol., 26, 1019, 10.1038/nbt.1490 Einav, 2008, The nucleotide binding motif of hepatitis C virus NS4B can mediate cellular transformation and tumor formation without Ha-ras co-transfection, Hepatology, 47, 827, 10.1002/hep.22108 Elazar, 2004, An N-terminal amphipathic helix in hepatitis C virus (HCV) NS4B mediates membrane association, correct localization of replication complex proteins, and HCV RNA replication, J. Virol., 78, 11393, 10.1128/JVI.78.20.11393-11400.2004 El-Hage, 2003, Replication of hepatitis C virus RNA occurs in a membrane-bound replication complex containing nonstructural viral proteins and RNA, J. Gen. Virol., 84, 2761, 10.1099/vir.0.19305-0 Gosert, 2003, Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons, J. Virol., 77, 5487, 10.1128/JVI.77.9.5487-5492.2003 Gouttenoire, 2009, Identification of a novel determinant for membrane association in hepatitis C virus nonstructural protein 4B, J. Virol., 83, 6257, 10.1128/JVI.02663-08 Hugle, 2001, The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein, Virology, 284, 70, 10.1006/viro.2001.0873 Ishido, 1998, Complex formation of NS5B with NS3 and NS4A proteins of hepatitis C virus, Biochem. Biophys. Res. Commun., 244, 35, 10.1006/bbrc.1998.8202 Jones, 2009, The hepatitis C virus NS4B protein can trans-complement viral RNA replication and modulates production of infectious virus, J. Virol., 83, 2163, 10.1128/JVI.01885-08 Kadoya, 2005, Nonstructural proteins 4A and 4B of hepatitis C virus transactivate the interleukin 8 promoter, Microbiol. Immunol., 49, 265, 10.1111/j.1348-0421.2005.tb03728.x Kato, 1990, Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis, Proc. Natl. Acad. Sci. U. S. A., 87, 9524, 10.1073/pnas.87.24.9524 Kato, 2002, Hepatitis C virus NS4A and NS4B proteins suppress translation in vivo, J. Med. Virol., 66, 187, 10.1002/jmv.2129 Koch, 1999, Modulation of hepatitis C virus NS5A hyperphosphorylation by nonstructural proteins NS3, NS4A, and NS4B, J. Virol., 73, 7138, 10.1128/JVI.73.9.7138-7146.1999 Konan, 2003, Nonstructural protein precursor NS4A/B from hepatitis C virus alters function and ultrastructure of host secretory apparatus, J. Virol., 77, 7843, 10.1128/JVI.77.14.7843-7855.2003 Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Liefhebber, 2009, Hepatitis C virus NS4B carboxy terminal domain is a membrane binding domain, Virol. J., 6, 62, 10.1186/1743-422X-6-62 Lohmann, 1999, Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line, Science, 285, 110, 10.1126/science.285.5424.110 Lundin, 2003, Topology of the membrane-associated hepatitis C virus protein NS4B, J. Virol., 77, 5428, 10.1128/JVI.77.9.5428-5438.2003 Lundin, 2006, Dual topology of the processed hepatitis C virus protein NS4B is influenced by the NS5A protein, J. Gen. Virol., 87, 3263, 10.1099/vir.0.82211-0 Miller, 1990, Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups, Proc. Natl. Acad. Sci. U. S. A., 87, 2057, 10.1073/pnas.87.6.2057 Moradpour, 2004, Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes, J. Virol., 78, 7400, 10.1128/JVI.78.14.7400-7409.2004 Paredes, 2008, A genetic interaction between hepatitis C virus NS4B and NS3 is important for RNA replication, J. Virol., 82, 10671, 10.1128/JVI.00875-08 Pietschmann, 2001, Characterization of cell lines carrying self-replicating hepatitis C virus RNAs, J. Virol., 75, 1252, 10.1128/JVI.75.3.1252-1264.2001 Qu, 2001, Isolation and characterization of noncytopathic pestivirus mutants reveals a role for nonstructural protein NS4B in viral cytopathogenicity, J. Virol., 75, 10651, 10.1128/JVI.75.22.10651-10662.2001 Rouille, 2006, Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus, J. Virol., 80, 2832, 10.1128/JVI.80.6.2832-2841.2006 Stone, 2007, Participation of rab5, an early endosome protein, in hepatitis C virus RNA replication machinery, J. Virol., 81, 4551, 10.1128/JVI.01366-06 Targett-Adams, 2008, Visualization of double-stranded RNA in cells supporting hepatitis C virus RNA replication, J. Virol., 82, 2182, 10.1128/JVI.01565-07 Thompson, 2009, Biochemical characterization of recombinant hepatitis C virus nonstructural protein 4B: evidence for ATP/GTP hydrolysis and adenylate kinase activity, Biochemistry, 48, 906, 10.1021/bi801747p Tong, 2002, Physical interaction between hepatitis C virus NS4B protein and CREB-RP/ATF6beta, Biochem. Biophys. Res. Commun., 299, 366, 10.1016/S0006-291X(02)02638-4 Yanagi, 1998, Transcripts of a chimeric cDNA clone of hepatitis C virus genotype 1b are infectious in vivo, Virology, 244, 161, 10.1006/viro.1998.9092 Yu, 2006, Palmitoylation and polymerization of hepatitis C virus NS4B protein, J. Virol., 80, 6013, 10.1128/JVI.00053-06 Zheng, 2005, Hepatitis C virus non-structural protein NS4B can modulate an unfolded protein response, J. Microbiol., 43, 529