Sự supress hoạt động EEG động kinh cấp tính do hóa chất gây ra bằng siêu âm hội tụ
Tóm tắt
Động kinh là một rối loạn thần kinh phổ biến, do sự tăng hoạt bất thường và không kiểm soát của các nơron. Chúng tôi đã điều tra khả năng sử dụng bức xạ xung động thấp, siêu âm hội tụ (FUS) để ức chế không xâm lấn hoạt động động kinh trong một mô hình động vật (chuột cống), được gây ra bởi tiêm pentylenetetrazol (PTZ) vào khoang bụng.
Sau khi bắt đầu xuất hiện cơn động kinh, FUS được truyền qua hộp sọ vào não hai lần, mỗi lần ba phút, trong khi tiến hành theo dõi điện não đồ (EEG). Một máy phát siêu âm hình cầu có khí đệm (đường kính: 6 cm; bán kính cong: 7 cm) hoạt động ở tần số cơ bản 690 KHz đã được sử dụng để truyền một chuỗi xung siêu âm kéo dài 0.5 msec với tần suất lặp lại 100 Hz đến các khu vực đồi thị của não. Cường độ âm (130 mW/cm2) được sử dụng trong thí nghiệm nằm trong khoảng an toàn cho hình ảnh siêu âm lâm sàng. Sự xuất hiện các đợt nổ EEG động kinh từ chuột bị động kinh đã giảm đáng kể sau khi truyền siêu âm khi so với trạng thái động kinh trước khi truyền. Nhóm kiểm soát bị kích thích PTZ mà không nhận được bất kỳ liệu pháp siêu âm nào cho thấy một số lượng đợt nổ tín hiệu EEG động kinh duy trì. Các động vật trải qua liệu pháp siêu âm cũng cho thấy hành vi động kinh ít nghiêm trọng hơn, được đánh giá bằng điểm số Racine. Phân tích mô học xác nhận rằng việc truyền siêu âm không gây tổn thương cho mô não.
Các kết quả này đã chỉ ra rằng việc truyền siêu âm xung lực thấp FUS đã ức chế số lượng đợt nổ tín hiệu động kinh trong mô hình động kinh cấp tính trên động vật. Do tính chất không xâm lấn và chọn lọc về mặt không gian, FUS có thể mở ra những triển vọng mới cho một phương pháp điều trị động kinh không xâm lấn.
Từ khóa
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