Fluorescence Intensity Calibration Using the Raman Scatter Peak of Water

Applied Spectroscopy - Tập 63 Số 8 - Trang 936-940 - 2009
Anders J. Lawaetz1,2, Colin A. Stedmon1,2
1Department of Marine Ecology, National Environmental Research Institute, University of Aarhus, Frederiksborgvej 399, Roskilde, 4000 Denmark (C.A.S.)
2Dept. Food Science, Faculty of Life Sciences, University Copenhagen, Rolighedsvej 30, DK-1958, Frederiksberg, Denmark (A.J.L.); and Department of Marine Ecology, National Environmental Research Institute, University of Aarhus, Frederiksborgvej 399, Roskilde, 4000 Denmark (C.A.S.)

Tóm tắt

Fluorescence data of replicate samples obtained from different fluorescence spectrometers or by the same spectrometer but with different instrument settings can have great intensity differences. In order to compare such data an intensity calibration must be applied. Here we explain a simple calibration method for fluorescence intensity using only the integrated area of a water Raman peak. By applying this method to data from three different instruments, we show that it is possible to remove instrument-dependent intensity factors, and we present results on a unified scale of Raman units. The method presented is a rapid and simple approach suitable for routine measurements with no need for hazardous chemicals.

Từ khóa


Tài liệu tham khảo

10.1021/cr050019q

10.1016/S0003-2670(00)84422-0

10.1038/348432a0

10.1007/978-0-387-46312-4

10.1007/s10895-005-2629-9

10.1063/1.2715952

10.1366/000370206777886973

Stedmon C. A., Ryder A. G., Harrit N. and Bro R, Pure Appl. Chem., paper submitted (2009).

Yentsch C. S., 1963, Deep-Sea Res., 10, 221

Velapoldi R. A. and Mielenz K. D, NBS Spec. Publ. *260–64 (1980).

10.1016/0304-4203(93)90116-6

10.1016/j.jlumin.2007.08.003

10.1016/S0304-4203(03)00072-0

10.1016/0967-0637(94)90048-5

10.1364/AO.36.002686

10.1023/B:JOFL.0000008059.74052.3c

Thông tin
Thông tin xuất bản

Applied Spectroscopy

Tập 63 Số 8

936-940

Thông tin tác giả