FluRox based biosensors
Tóm tắt
A biosensor is an integrated device of biomaterials and electronic components which detects physiological change or physico-chemical response. The efforts towards the development of a supersensitive FluoRox biosensor are discussed in this paper. FluoRox principle is based on the novel concept of monitoring redox events in vitro and in vivo by fluorescence detection based on forster resonance energy transfer (FRET). Unlike conventional electrochemical biosensors fluorescence based sensors has the advantage of higher sensitivity which under suitable conditions can detect single molecules. Thus a highly sensitive and a miniaturized device is aimed at, which will enable the detection of trace amounts of pollutants and the detection of diseases at an early stage.
Tài liệu tham khảo
Boutelle MG, Hill HAO, Berners M, John R, Dobson PD, Leigh P (1996) New technologies for amperometric biosensors. J Mol Recognit 9:664–671. doi:10.1002/(SICI)1099-1352(199634/12)9:5/6<664::AID-JMR318>3.0.CO;2-U
Kuznetsova S, Zauner G, Schmauder R, Mayboroda O, Deelder A, Aartsma TA, Canters GW (2006) A Förster-resonance-energy-transfer based method for fluorescence detection of the protein redox state. Anal Biochem 350:52–60. doi:10.1016/j.ab.2005.11.036
Kuznetsova S, Zauner G, Aartsma TA, Engelkamp H, Hatzakis N, Rowan AE, Nolte RJM, Christianen PCM, Canters GW (2008) The enzyme mechanism of nitrite reductase studied at single-molecule level. Proc Natl Acad Sci USA 105:3250–3255. doi:10.1073/pnas.0707736105
Strianese M, Zauner G, Tepper AWJW, Bubacco L, Breukink E, Aartsma TA, Canters GW, Tabares LC (2009) A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability. Anal Biochem 385:242–248. doi:10.1016/j.ab.2008.11.017
Zauner Z, Lonardi E, Bubacco L, Aartsma TJ, Canters GW, Tepper AWJW (2007) Tryptophan-to-dye fluorescence energy transfer applied to oxygen sensing using type-3 copper proteins. Chem Eur J 13:7085–7090. doi:10.1002/chem.200601806