Flow cytometric analysis of CD4+ T cell reactivation following anti-PD1 immunotherapy in a transgenic mouse model

Bending, 2018, A timer for analyzing temporally dynamic changes in transcription during differentiation in vivo, J. Cell Biol., 217, 2931, 10.1083/jcb.201711048 Burton, 2014, Sequential transcriptional changes dictate safe and effective antigen-specific immunotherapy, Nat. Commun., 5, 4741, 10.1038/ncomms5741 Elliot, 2021, Antigen and checkpoint receptor engagement recalibrates T cell receptor signal strength, Immunity, 54, 2481, 10.1016/j.immuni.2021.08.020 Fairchild, 1993, An autoantigenic T cell epitope forms unstable complexes with class II MHC: a novel route for escape from tolerance induction, Int. Immunol., 5, 1151, 10.1093/intimm/5.9.1151 Forsyth, 2000, A panel of monoclonal antibodies to ovine placental lactogen, J. Endocrinol., 165, 435, 10.1677/joe.0.1650435 Jennings, 2020, Nr4a1 and Nr4a3 reporter mice are differentially sensitive to t cell receptor signal strength and duration, Cell Rep., 33, 108328, 10.1016/j.celrep.2020.108328 Jennings, 2021, Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry, STAR Protoc., 2, 100284, 10.1016/j.xpro.2020.100284 Subach, 2009, Monomeric fluorescent timers that change color from blue to red report on cellular trafficking, Nat. Chem. Biol., 5, 118, 10.1038/nchembio.138