First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam
Tóm tắt
Entomopathogenic nematodes (EPNs) are evidently a useful nematode group for the biocontrol of insect pests. It is well known that efficacy of different EPN strains, even belonging to the same species, can be significantly varied in different localities. Therefore, exploring EPNs and testing their efficacy in various ecological regions is of crucial importance to find out more efficient EPN strains. On the other hand, this practice is also needed to enhance the knowledge on diversity and distribution model of EPNs over the world. In this study, a species belonging to the genus Steinernema, S. surkhetense, has been characterized for the first time in Vietnam based on morphological and molecular characterizations. Morphological characterizations of infective juveniles, the first and second-generation adults, and molecular characterization of D2-D3 expansion segment of 28S rRNA region were given. Molecular phylogeny of the genus Steinernema was also provided. In addition, the study showed that the lethal efficacy of this local strain to larvae of Galleria mellonella L. was relatively higher than other reported EPN strains in Vietnam. The Vietnamese EPN population found in this study was determined to be conspecific with S. surkhetense, revealed its new distribution in Vietnam. Besides, detailed morphological and molecular characterizations of it was provided with small variations compared to other populations in the world, and its relatively high lethal efficacy on larvae of G. mellonella implied that this strain can be potentially a useful strain for biological control of insect pests.
Tài liệu tham khảo
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402. https://doi.org/10.1093/nar/25.17.3389
Bedding RA, Akhurst RJ (1975) A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica 21:109–116
Bhat AH, Sharma L, Chaubey AK (2020) Characterisation of Steinernema surkhetense and its symbiont Xenrorhabdus stockiae and a note on its geographical distribution. Egypt Acad J Biol Sci A Entomol 13:105–122
De Doucet MMA, Bertolotti MA, Giayetto AL, Miranda MB (1999) Host range, specificity, and virulence of Steinernema feltiae, Steinernema rarum, and Heterorhabditis bacteriophora (Steinernematidae and Heterorhabditidae) from Argentina. J Invertebr Pathol 73:237–242
De Ley P, Felix MA, Frisse L, Nadler S, Sternberg P, Thomas WK (1999) Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1:591–612
Didiza, L, Lephoto, TE & Gray, VM (2021) Morphological and molecular phylogenetic description of Steinernema batswanae n. sp. (Rhabditida: Steinernematidae): a new species of an entomopathogenic nematode from South Africa. Arch Phytopathol Plant Prot. https://doi.org/10.1080/03235408.2021.1931648.
Hunt DJ, Nguyen KB (2016) Advances in entomopathogenic nematode taxonomy and phylogeny. Brill, Leiden, The Netherlands
Ibm Corp (2017) IBM SPSS Statistics for Windows, Version 25.0. IBM Corp, Armonk, NY
Khatri-Chhetri HB, Waeyenberge L, Spiridonov S, Manandhar HK, Moens M (2011) Two new species of Steinernema Travassos, 1927 with short infective juveniles from Nepal. Russ J Nematol 19:53–74
Laznik Ž, Tóth T, Lakatos T, Vidrih M, Trdan S (2010) Control of the Colorado potato beetle (Leptinotarsa decemlineata [Say]) on potato under field conditions: a comparison of the efficacy of foliar application of two strains of Steinernema feltiae (Filipjev) and spraying with thiametoxam. J Plant Dis Prot 117:129–135
Laznik Z, Trdan S (2011) Entomopathogenic nematodes (Nematoda: Rhabditida) in Slovenia: from tabula rasa to implementation into crop production systems. In: Perveen F (ed) Insecticides - advances in integrated pest management. InTech, Rijeka, pp 627–656
Nguyen HT, Nguyen TD, Le TML, Trinh QP (2020) First report of Xiphinema hunaniense Wang & Wu, 1992 (Nematoda: Longidoridae) in Vietnam. J Nematol 52:e2020–e2078. https://doi.org/10.21307/jofnem-2020-078
Nguyen KB, Hunt DJ (2007) Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts. Brill, Leiden, The Netherlands
Nguyen NC (2008) Entomopathogenic nematodes in Vietnam (in Vietnamese). Publishing House for Science and Technology, Hanoi, Vietnam
Phan KL, Mráček Z, Půža V, Nermut J, Jarošová A (2014) Steinernema huense sp. n., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Vietnam. Nematology 16:761–775
Seinhorst JW (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4:67–69. https://doi.org/10.1163/187529259x00381