Fine structure and the localization of the nitrite oxidizing system inNitrobacter winogradskyl

Antonie van Leeuwenhoek - Tập 34 - Trang 483-494 - 2013
H.-C. Tsien1, R. Lambert1, H. Laudelout1
1Faculté des Sciences Agronomiques, Université de Louvain, Louwain, Belgium

Tóm tắt

The existence of a membrane system located at one of the poles of the cell is confirmed in the case ofNitrobacter winogradskyi. Additional information on the structure of this system is presented. Elementary particles with diameters of 90 – 100 Å are embedded on a layer of membrane protein. Identification of the membrane system as the location of nitrite oxidation was achieved by a combination of differential spectrophotometry and electron microscopic study of membrane fragments separated by density gradient centrifugation. The cytochrome chain responsible for nitrite oxidation appears to be located in fragments of the membrane system. Prominent morphological features ofNitrobacter winogradskyi are the high contents in poly-β-hydroxybutyrate and polyphosphate granules.

Tài liệu tham khảo

Abram, D. 1965. Electron microscope observations on intact cells, protoplasts, and the cytoplasmic membrane ofBacillus stearothermophilus. J. Bacteriol.89: 855–873. Boon, B. andLaudelout, H. 1962. Kinetics of nitrite oxidation byNitrobacter winogradskyi. Biochem. J.85: 440–447. Brenner, S. andHorne, R. W. 1959. A negative staining method for high resolution electron microscopy of viruses. Biochim. Biophys. Acta.34: 103–110. Chance, B., Parsons, D. F. andWilliams, G. R. 1964. Cytochrome content of mitochondria stripped of inner membrane structure. Science143: 136–139. Conti, S. F. andHirsch, P. 1965. Biology of budding bacteria. III. Fine structure ofRhodomicrobium andHyphomicrobium spp. J. Bacteriol.89: 503–512. Fernandez-Moran, H., Oda, T., Blair, P. V. andGreen, D. E. 1964. A macromolecular repeating unit of mitochondrial structure and function. J. Cell Biol.22:63–100. Frasca, J. M. andParks, V. R. 1965. A routine technique for double-staining ultrathin sections using uranyl and lead salts. J. Cell Biol.25: 157–161. Green, D. E. andPerdue, J. F. 1966. Correlation of mitochondrial structure and function. Ann. N. Y. Acad. Sci.137: 667–684. Huxley, H. E. andZubay, G. 1960. Electron microscope observations on the structure of microsomal particles fromEscherichia coli. J. Mol. Biol.2: 10–18. Law, J. H. andSlepecky, R. A. 1961. Assay of poly-β-hydroxybutyric acid. J. Bacteriol.82: 33–36. Lees, H. 1962. Some thoughts on the energetics of chemosynthesis. Bacteriol. Rev.26: 165–167. Lundgren, D. G., Pfister, R. M. andMerrick, J. M. 1964. Structure of poly-β-hydroxybutyric acid granules. J. Gen. Microbiol.34: 441–446. Millonig, G. 1961. A modified procedure for lead staining of thin sections. J. Biophys. Biochem. Cytol.11: 736–739. McHlethaler, K. 1966. Biochemistry of Chloroplasts, Vol. 1. T. H. Goodwin, [ed.]. Academic Press, Inc., New York. Mühlethaler, K., Moor, H. andSzarkowski, H. W. 1965. The ultrastructure of the chloroplast lamellae. Planta67: 305–323. Murray, R. G. E. 1963. General physiology of cell specialization. D. Mazia and A. Tyler, [ed.]. McGraw-Hill Book Co., Inc., New York. Murray, R. G. E. andWatson, S. W. 1965. Structure ofNitrosocystis oceanus and comparison withNitrosomonas andNitrobacter. J. Bacteriol.89: 1594–1609. Park, R. B. 1965. Substructure of chloroplast lamellae. J. Cell Biol.27: 151–161. Remsen, C. C., Valots, F. W. andWatson, S. W. 1967. Fine structure of the cytomembranes ofNitrosocystis oceanus. J. Bacteriol.94: 422–433. Ryter, A. etKellenberger, E. 1958. Étude au microscope électronique de plasmas contenant de l'acide deoxyribonucleique. 1. Les nucléoides des bactéries en croissance active. Z. Naturforsch.13b: 597–605. Starr, M. P. andSkerman, V. B. D. 1965. Bacterial diversity: The natural history of selected morphologically unusual bacteria. Ann. Rev. Microbiol.19: 407–454. Tobback, P. andLaudelout, H. 1966. La culture deNitrobacter en dialyse continue. Arch. Mikrobiol.54: 14–20. Van Gool, A. andLaudelout, H. 1966. The mechanism of nitrite oxidation byNitrobacter winogradskyi. Biochim. Biophys. Acta113: 41–50. Zavarzin, G. andLegunkova, R. 1959. The morphology ofNitrobacter winogradskyi. J. Gen. Microbiol.21: 186–190.