Field‐evolved resistance to Bt toxin Cry1Ac in the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), from India

Pest Management Science - Tập 67 Số 8 - Trang 898-903 - 2011
S. Dhurua1, G. T. Gujar1
1Division of Entomology, Indian Agricultural Research Institute, New Delhi, India

Tóm tắt

Abstract

BACKGROUND: The pink bollworm is one of the most destructive pests of cotton. Transgenic cotton producing Bt toxin Cry1Ac or a combination of Cry1Ac and Cry2Ab2 has been used effectively against this pest. However, some other insects have evolved resistance to Bt toxins in the field. During the 2007–2008 and 2008–2009 seasons, pink bollworm populations in India were surveyed to evaluate their responses to Cry1Ac and seed powder containing Cry1Ac and Cry2Ab2.

RESULTS: The results provide evidence that resistance to Cry1Ac had evolved by 2008 in a population sampled from non‐Bt cotton in the Amreli district of Gujarat in western India. The median lethal concentration of Cry1Ac for five‐day‐old larvae (LC50) was significantly higher for insects derived in 2008 from Amreli than for any of the other field populations tested from four locations in India. For Cry1Ac, the mean LC50 for the strain derived from Amreli in 2008 was 44 times higher than for the most susceptible population. However, for seed powder of Bollgard II containing primarily Cry2Ab2, the 2008 Amreli population was only slightly less susceptible than the most susceptible population.

CONCLUSIONS: The data reported here constitute the first evidence of field‐evolved resistance of pink bollworm to Cry1Ac. This initial evidence spurred more extensive evaluations during the 2009–2010 growing season, which confirmed field‐evolved resistance to Cry1Ac in Amreli. The lack of cross‐resistance to Cry2Ab2 suggests that plants producing this toxin are likely to be more effective against resistant populations than plants producing only Cry1Ac. Copyright © 2011 Society of Chemical Industry

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Tài liệu tham khảo

10.1016/B978-012432555-5/50006-3

10.1002/arch.10117

10.1128/MMBR.62.3.807-813.1998

CrickmoreN ZeiglerDR SchnepfE Van RieJ LereclusD BaumJ et al Bacillus thuringiensisToxin Nomenclature.[Online]. (2009). Available:http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/[21 February 2011].

Entwistle P, 1993, Bacillus thuringiensis: an Environmental Pesticide, Theory and Practice, 311

10.1016/S0261-2194(99)00028-9

10.1126/science.229.4709.193

10.1146/annurev.ento.47.091201.145234

10.1093/jee/83.5.1671

10.1098/rspb.2003.2497

Luttrell RG, 2004, Proceedings of Beltwide Cotton Conferences, 1373

10.1007/978-1-4020-8373-0_2

10.1080/02571862.2007.10634798

10.1002/ps.1849

JamesC Global status of commercialized biotech/GM crops. ISAAA Brief No. 41/42 ISAAA Ithaca NY (2009).

Bambawale OM, 2004, Performance of Bt cotton (MECH‐162) under integrated pest management in farmers' participatory field trial in Nanded district, Central India, Curr Sci, 86, 1628

10.1126/science.1080609

Rao NC, 2009, Socio‐economic impact of transgenic cotton, Agril Econ Res Rev, 22, 461

Gujar GT, 2000, Spatial and temporal variation in susceptibility of American bollworm Helicoverpa armigera (Hübner) to Bacillus thuringiensis var. kurstaki, Curr Sci, 78, 995

10.1016/j.jip.2007.03.011

10.1016/j.cropro.2007.08.008

10.1016/j.cropro.2008.12.001

10.1017/S1742758409990129

Patil BV, 2007, Status of pink bollworm, Pectinophora gossypiella (Saunders), on cotton at Raichur, Karnataka, J Cotton Res Dev, 21, 224

Kumar S, 2005, Incidence of bollworms in promising cultivars of cotton in Haryana, J Cotton Res Dev, 19, 227

10.1093/jee/89.1.230

Simmons AL, 1998, Proceedings of Beltwide Cotton Conferences, Vol. 2, 1025

Henneberry TJ, 2000, Seasonal pink bollworm, Pectinophora gossypiella (Saunders), infestations of transgenic and non‐transgenic cottons, Southwest Entomol, 25, 273

10.1128/AEM.68.8.3790-3794.2002

10.1073/pnas.0436708100

Jeughale GS, 2007, Effect of Bt cotton hybrids as one of the components of IPM on pink bollworm incidence under rainfed situation, Crop Res, 34, 206

Nadaf ARM, 2007, Correlation between pink bollworm infestation and weather parameters in Bt and non‐Bt cotton, Karnataka J Agric Sci, 20, 752

Bartlett AC, 1995, Proceedings of Beltwide Cotton Conferences., 766

10.1073/pnas.97.24.12980

Tabashnik BE, 2004, Shared genetic basis of resistance to Bt toxin Cry1Ac in independent strains of pink bollworm, J Econ Entomol, 97, 721, 10.1093/jee/97.3.721

10.1073/pnas.0507857102

10.1603/0022-0493-99.6.2125

10.1073/pnas.0901351106

Bartlett AC, 1985, Handbook of Insect Rearing, Vol. 2, 415

Paul AVN, 1987, Artificial diet for Pectinophora gossypiella and Earias vitella bollworms of cotton, Indian J Agric Sci, 57, 89

Patin AL, 1999, Proceedings of Beltwide Cotton Conferences., 991

Ross GES, 1987, Maximum Likelihood Programme.

Litchfield JT, 1949, A simplified method of evaluating dose–effect experiments, J Pharma Exp Ther, 96, 99

VennilaS BiradarVK SabeshMandBambawaleOM Know your cotton insect pest pink bollworm. Crop Protection Folder Series 7 CICR Nagpur India (2007).

UnnithanGC BrinkS WoodB CarriereY TabashnikB DennehyTJ et al Susceptibility of southwestern pink bollworm to CRY2Ab: baseline responses in 2001 and 2002. University of Arizona College of Agriculture Cotton Report 12pp. (2004).

DennehyTJ UnnithanGC HarpoldVS CarrièreYandTabashnikBE Susceptibility of pink bollworm toBttoxins Cry 1Ac and Cry2Ab2 in the southwestern USA in 2006. University of Arizona College of Agriculture Cotton Report 30pp. (2008).

10.1603/0022-0493-99.5.1525

10.1126/science.1146453

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Pest Management Science

Tập 67 Số 8

898-903

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