Lauren B. Carrington1,2, Bich Chau Nguyen Tran2, Le Thanh Hoang Nhat2, Tai Thi Hue Luong3, Truong Thanh Nguyen2, Phong Thanh Nguyen3,2,4, Nguyễn Văn Vĩnh Châu3,2,4, Nguyen Thi Cam Huong3,2,4, Trung Vu3,2, Long Thi Vo2, Dui Thi Le2, Nhu Tuyet Vu3,2, Giang Nguyen3,2,4, Hung Quoc Luu5, Anh Duc Dang5, Timothy P. Hurst6, Scott L. O’Neill6, Vi Tran2, Duong Thi Hue Kien2, Nguyet Minh Nguyen2, Marcel Wolbers2, Bridget Wills2, Cameron P. Simmons1,6,2
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Melbourne, VIC 3010, Australia;
2Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Programme, District 5, Ho Chi Minh City, Vietnam;
3Hospital for Tropical Diseases, District 5, Ho Chi Minh City, Vietnam;
4University of California, Irvine, CA
5National Institute of Hygiene and Epidemiology, Hai Ba Trung District, Hanoi, Vietnam; and
6Institute for Vector Borne Disease, Monash University, Clayton, Melbourne, VIC 3168, Australia
Tóm tắt
Significance
In laboratory experiments,
Wolbachia
(
w
Mel strain)-infected
Aedes aegypti
are refractory to disseminated arboviral infections. Yet previous characterizations of
w
Mel-mediated blocking have not considered several biologically and ecologically important factors likely to influence the virus–mosquito interaction. After direct feeding on 141 viremic dengue patients, we demonstrate
w
Mel lowers dengue virus (DENV) transmission potential and lengthens the extrinsic incubation period. Subsequently, using established field populations of wild-type and
w
Mel-infected
Ae. aegypti
, we compared field- versus laboratory-rearing conditions on mosquito susceptibility to disseminated DENV infection. The magnitude of
w
Mel-mediated virus blocking was even greater when mosquitoes developed under field conditions. These clinically and ecologically relevant findings support
Wolbachia
introgression into
Ae. aegypti
populations as a biocontrol method to reduce the transmission of DENV and other arboviruses.
