Fed-batch mixotrophic cultivation of Chlamydomonas reinhardtii for high-density cultures

Harris, 2001, Chlamydomonas as a model organism, Annu. Rev. Plant Biol., 52, 363, 10.1146/annurev.arplant.52.1.363 Merchant, 2007, The Chlamydomonas genome reveals the evolution of key animal and plant functions, Science, 318, 245, 10.1126/science.1143609 Harris, 2009 Rasala, 2015, Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses, Photosynth. Res., 123, 227, 10.1007/s11120-014-9994-7 Scranton, 2015, Chlamydomonas as a model for biofuels and bio-products production, Plant J., 82, 523, 10.1111/tpj.12780 Murbach, 2018, A toxicological evaluation of Chlamydomonas reinhardtii, a green algae, Int. J. Toxicol., 37, 53, 10.1177/1091581817746109 Barrera, 2013, High-value recombinant protein production in microalgae, 2, 532 Griesbeck, 2006, Chlamydomonas reinhardtii, Mol. Biotechnol., 34, 213, 10.1385/MB:34:2:213 Scaife, 2015, Establishing Chlamydomonas reinhardtii as an industrial biotechnology host, Plant J., 82, 532, 10.1111/tpj.12781 Specht, 2010, Micro-algae come of age as a platform for recombinant protein production, Biotechnol. Lett., 32, 1373, 10.1007/s10529-010-0326-5 Tran, 2013, Production of immunotoxins in algal chloroplasts, Proc. Natl. Acad. Sci., 110, E15, 10.1073/pnas.1214638110 Mayfield, 2007, Chlamydomonas reinhardtii chloroplasts as protein factories, Curr. Opin. Biotechnol., 18, 126, 10.1016/j.copbio.2007.02.001 Manuell, 2007, Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast, Plant Biotechnol. J., 5, 402, 10.1111/j.1467-7652.2007.00249.x Demain, 2009, Production of recombinant proteins by microbes and higher organisms, Biotechnol. Adv., 27, 297, 10.1016/j.biotechadv.2009.01.008 Shin, 2016, CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii, Sci. Rep., 6 Venter, 2007, Synthetic promoters: genetic control through cis engineering, Trends Plant Sci., 12, 118, 10.1016/j.tplants.2007.01.002 Borowitzka, 2013 Chen, 2011, Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review, Bioresour. Technol., 102, 71, 10.1016/j.biortech.2010.06.159 Schoepp, 2014, System and method for research-scale outdoor production of microalgae and cyanobacteria, Bioresour. Technol., 166, 273, 10.1016/j.biortech.2014.05.046 Szyjka, 2017, Evaluation of phenotype stability and ecological risk of a genetically engineered alga in open pond production, Algal Res., 24, 378, 10.1016/j.algal.2017.04.006 Bumbak, 2011, Best practices in heterotrophic high-cell-density microalgal processes: achievements, potential and possible limitations, Appl. Microbiol. Biotechnol., 91, 31, 10.1007/s00253-011-3311-6 Perez-Garcia, 2011, Heterotrophic cultures of microalgae: metabolism and potential products, Water Res., 45, 11, 10.1016/j.watres.2010.08.037 Gorman, 1965, Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi, Proc. Natl. Acad. Sci., 54, 1665, 10.1073/pnas.54.6.1665 Chen, 1995, A strategy for high cell density culture of heterotrophic microalgae with inhibitory substrates, J. Appl. Phycol., 7, 43, 10.1007/BF00003548 Chen, 1996, Heterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture, Process Biochem., 31, 601, 10.1016/S0032-9592(96)00006-4 Sack, 1994, Isolation of four new strains of Chlamydomonas reinhardtii (Chlorophyta) from soil samples, J. Phycol., 30, 770, 10.1111/j.0022-3646.1994.00770.x Barnes, 2005, Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes, Mol. Gen. Genomics., 274, 625, 10.1007/s00438-005-0055-y Rasala, 2010, Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii, Plant Biotechnol. J., 8, 719, 10.1111/j.1467-7652.2010.00503.x Rasala, 2011, Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5′ untranslated region optimization, Plant Biotechnol. J., 9, 674, 10.1111/j.1467-7652.2011.00620.x Kropat, 2011, A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii, Plant J., 66, 770, 10.1111/j.1365-313X.2011.04537.x Zhu, 1997, Determination of biomass dry weight of marine microalgae, J. Appl. Phycol., 9, 189, 10.1023/A:1007914806640 Kind, 2012, Qualitative analysis of algal secretions with multiple mass spectrometric platforms, J. Chromatogr. A, 1244, 139, 10.1016/j.chroma.2012.04.074 Fiehn, 2008, Quality control for plant metabolomics: reporting MSI-compliant studies, Plant J., 53, 691, 10.1111/j.1365-313X.2007.03387.x Miller Boyle, 2009, Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii, BMC Syst. Biol., 3, 4, 10.1186/1752-0509-3-4 Moore, 2001, Membrane lipid biosynthesis in Chlamydomonas reinhardtii. In vitro biosynthesis of diacylglyceryltrimethylhomoserine, Plant Physiol., 125, 423, 10.1104/pp.125.1.423 Sharma, 2012, High lipid induction in microalgae for biodiesel production, Energies, 5, 1532, 10.3390/en5051532 Husic, 1986, Effect of osmotic stress on carbon metabolism in Chlamydomonas reinhardtii, Plant Physiol., 82, 594, 10.1104/pp.82.2.594 Mastrobuoni, 2012, Proteome dynamics and early salt stress response of the photosynthetic organism Chlamydomonas reinhardtii, BMC Genomics, 13, 215, 10.1186/1471-2164-13-215