Fecal short‐chain fatty acid concentrations and dysbiosis in dogs with chronic enteropathy

Journal of Veterinary Internal Medicine - Tập 33 Số 4 - Trang 1608-1618 - 2019
Yasushi Minamoto1, Tomomi Minamoto1, Anitha Isaiah1, Panpicha Sattasathuchana1, Agostino Buono1, Venkat R. Rangachari1, Isaac H. McNeely1, Jonathan A. Lidbury1, Jörg M. Steiner1, Jan S. Suchodolski1
1Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences, Texas A&M University College Station Texas

Tóm tắt

AbstractBackgroundAccumulating evidence shows an important relationship between the gastrointestinal (GI) microbiota and host health. Microbial metabolites are believed to play a critical role in host‐microbial interactions. Short‐chain fatty acids (SCFAs) are major end products of bacterial carbohydrate fermentation in the intestinal tract. Decreased concentrations of SCFAs have been observed in humans with GI disease. However, large‐scale clinical data in dogs are lacking.Hypothesis/ObjectiveTo evaluate fecal concentrations of SCFAs and the fecal microbiota in healthy control (HC) dogs and dogs with chronic enteropathy (CE).AnimalsForty‐nine privately owned HC dogs and 73 dogs with CE.MethodsProspective cohort study. Fecal concentrations of SCFAs were measured using gas chromatography/mass spectrometry. Illumina sequencing and quantitative real‐time polymerase chain reaction were utilized to evaluate the fecal microbiota.ResultsFecal concentrations (median [range] μmol/g of dry matter) of acetate were lower (P = .03) in dogs with CE (185.8 [20.1‐1042.1]) than in HC dogs (224.0 [87.7‐672.8]). Propionate were also lower (P < .001) in dogs with CE (46.4 [0.4‐227.9]) than in HC dogs (105.9 [1.6‐266.9]). Moreover, total SCFAs were lower (P = .005) in dogs with CE (268.1 [21.8‐1378.2]) than in HC dogs (377.2 [126.6‐927.0]). Dysbiosis in dogs with CE was characterized by decreased bacterial diversity and richness, distinct microbial community clustering compared with that in HC dogs, and a higher dysbiosis index.Conclusions and Clinical ImportanceDogs with CE had an altered fecal SCFA concentration accompanied by significant changes of the fecal microbiota.

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Tài liệu tham khảo

10.1038/ni.2640

10.1146/annurev-physiol-021909-135817

10.1152/physrev.1990.70.2.567

10.1016/S1098-612X(03)00002-0

Kamath PS, 1987, Short‐chain fatty acids stimulate motility of the canine ileum, Am J Physiol, 253, 427

10.1021/pr060470d

10.3945/jn.109.104380

10.1126/science.1241165

10.1053/j.gastro.2013.04.056

10.1111/j.1365-2672.1991.tb04442.x

10.1016/B978-0-12-407672-3.00003-4

10.1111/mmi.12149

10.1046/j.1365-2958.2002.03268.x

10.3109/00365529409094863

10.1186/s13099-014-0044-y

10.1038/nature09646

10.2527/jas.2008-1659

10.1017/S0007114511005927

10.1111/j.1939-1676.2007.tb03011.x

10.1016/S1570-0232(02)00827-9

10.1016/j.vetmic.2014.10.005

10.1080/19490976.2014.997612

10.1111/j.1574-6941.2008.00521.x

10.1093/femsec/fix136

10.1371/journal.pone.0108729

10.1038/nmeth.f.303

10.1128/AEM.03006-05

10.1371/journal.pone.0127259

10.1128/AEM.72.1.946-949.2006

10.1016/j.jnutbio.2010.07.009

10.1016/j.nutres.2008.02.012

10.1177/0300985815591081

10.1186/gb-2012-13-9-r79

10.1172/JCI111847

10.1111/j.1365-2672.1992.tb04882.x

10.1042/bj1420401

10.1152/ajpgi.00282.2013

10.1038/ncomms4114

10.1128/JCM.00845-13

10.1136/gutjnl-2012-302578

10.1371/journal.pone.0051907

10.1371/journal.pone.0039333

10.5740/jaoacint.SGE_Macfarlane

10.1038/nrmicro3344

10.1186/1471-2180-9-210

10.1371/journal.pone.0107909

10.1186/1746-6148-9-201

10.1371/journal.pone.0094699

10.1038/nmicrobiol.2016.177

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Journal of Veterinary Internal Medicine

Tập 33 Số 4

1608-1618

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