Fatty acid profiles of “Pasteurella” piscicida: comparison with other fish pathogenic gram-negative bacteria
Tóm tắt
The fatty acid methyl ester (FAME) profiles of “Pasteurella” piscicida were determined by gas chromatography and subjected to numerical analysis in comparison with those obtained for Vibrio anguillarum, Aeromonas salmonicida and Pasteurella species of clinical origin. The bacterial species studied shared important characteristics with respect to their FAME content: in all of them the saturated and unsaturated fatty acids of 16 carbon atoms were the predominant fatty acids. However, distinguishing features could be detected for each pathogen. Using either single linkage or complete linkage algorithms, strains were divided into four phena that corresponded to the different species, but showed a high degree of correlation among them. Although single linkage discriminated strains better within each phenum, complete linkage was more useful to establish the relationships among clusters. The results obtained support the idea that “Pasteurella” piscicida is related to members of the genera Vibrio and Aeromonas and indicate the need for exhaustive genetic studies to clarify the taxonomic position of this fish pathogen.
Tài liệu tham khảo
Ajmal M, Hobbs BC (1967) Species of Corynebacterium and Pasteurella isolated from diseased salmon, trout and rudd. Nature 215:142–143
Baudin-Laurencin F, Pepin JF, Raymond JC (1991) First observation of an epizootic of pasteurellosis in farmed and wild fish of the French Mediterranean coasts (abstract). 5th International Conference of the European Association on Fish Pathologists, Budapest, Hungary, pp 17
Bøe B, Gjerde J (1980) Fatty acid patterns in the classification of some representatives of the families Enterobacteriaceae and Vibrionaceae. J Gen Microbiol 116:41–49
Bousfield IJ, Smith GL, Dando TR, Hobbs G (1983) Numerical analysis of total fatty acid profiles in the identification of coryneform, nocardioform and some other bacteria. J Gen Microbiol 119:375–394
Carter GR (1984) Genus I. Pasteurella Trevisan 1887. In: Krieg NR, Holt JG (eds) Bergey's manual of systematic bacteriology. Williams and Wilkins, Baltimore, pp 552–227
Ceschia G, Giorgetti G, Bovo G (1990) Grave epizoozia da Pasteurella piscicida in specie eurialine del Nord Adriatico. Boll Soc Ital Patol Ittica 4:11–17
De Ley J, Mannheim W, Mutters R, Piechulla K, Tytgat R, Segers P, Bisgaars M, Frederiksen W, Hinz K-H, Vanhoucke M (1990) Inter- and intrafamilial similarities of rRNA cistrons of the Pasteurellaceae. Int J Syst Bacteriol 40:126–137
Dewhirst FE, Paster BJ, Olsen I, Fraser GJ (1992) Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 174:2002–2013
Egusa S (1983) Disease problems in Japanese yellowtail Seriola quinqueradiata, culture: a review. In: Stuart JE (ed) Diseases of commercially important marine fish and shellfish. Conseil International pour l'Exploration de la Mer, Copenhagen, Denmark, pp 10–18
Grimes DJ, Jacobs D, Swartz PR, Brayton PR, Colwell RR (1993) Numerical taxonomy of gram-negative, oxidase-positive rods from carcharhinid sharks. Int J Syst Bacteriol 43:88–98
Haldeman DL, Amy PS (1993) Diversity within a colony morphotype: implications for ecological research. Appl Environ Microbiol 59:933–935
Herrera-Alcaraz E, Valero-Guillén P, Martín-Luengo F, Canteras-Jordana M (1993) Numerical analysis of fatty and mycolic acid profiles of Corynebacterium urealyticum and other related corynebacteria. Microbiología SEM 9:53–62
Håstein T, Bullock GL (1976) An acute septicaemic disease of brown trout (Salmo trutta) and Atlantic salmon (Salmo salar) caused by Pasteurella-like organism. J Fish Biol 8:23–26
Kimura M, Kitao T (1971) On the aetiological agent of “bacterial tuberculoidosis” of Seriola. Fish Pathol 6:8–14
Magariños B, Romalde JL, Bandín I, Fouz B, Toranzo AE (1992) Phenotypic, antigenic, and molecular characterization of Pasteurella piscicida strains isolated from fish. Appl Environ Microbiol 58:3316–3322
Moss CW, Wallace PL, Hollis DG, Weaver RE (1988) Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, in Moraxella species, Oligella urethralis, Acinetobacter, species, and Psychrobacter immobilis. J Clin Microbiol 26: 484–492
Muroga K, Sugiyama T, Ueki N (1977) Pasteurellosis in cultured black sea bream, Mylio macrocephalus. J Fac Fish Anim Husb, Hiroshima Univ 16:17–21
Nicolas JL, Gauthier G, Orsini L, Corre S (1994) Use of 16S rRNA sequences for taxonomy and detection of pathogenic bacteria (abstract). 6th International Colloquium of Pathology in Marine Aquaculture (PAMAQ 6), Montpellier, France. BIOCIM, Montpellier, pp 54
Paterson WD, Douey D, Desautels D (1980) Relationships between selected strains of typical and atypical Aeromonas salmonicida, Aeromonas hydrophila, and Haemophilus piscium. Can J Microbiol 26:588–598
Ruimy R, Breittmayer V, Elbaze P, Lafay B, Boussemart O, Gautheir M, Christen R (1994) Phylogenetic analysis and assessment of the gene Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences. Int J Syst Bacteriol 44:416–426
Simidu U, Egusa S (1972) A re-examination of the fish-pathogenic bacterium that had been reported as Pasteurella species. Bull Jpn Soc Sci Fish 38:803–812
Skerman VBD, McGowan V, Sneath PHA (1989). Approved list of bacterial names. American Society for Microbiology, Washington DC
Smith SK, Sutton DC, Fuerst JA, Reichelt JL (1991) Evaluation of genus Listonella and reassignment of Listonella damsela (Love et al.) MacDonell and Colwell to the genus Photobacterium as Photobacterium damsela comb. nov. with an emended description. Int J Syst Bacteriol 41:529–534
Snieszko SF, Bullock GL, Hollis E, Boone JG (1964) Pasteurella sp from a epizootic of white perch (Roccus americanus) in Chesapeake Bay tidewater areas. J Bacteriol 88:1814–1815
Sokal RR, Rohlf FJ (1981) Biometry. Freeman, New York
Toranzo AE, Barreiro S, Casal JF, Figueras A, Magariños B, Barja JL (1991) Pasteurellosis in gilthead seabream (Sparus aurata): first report in Spain. Aquaculture 99:1–15
Toranzo AE, Cutrín JM, Roberson B, Nuñez SA, Abell JM, Hetrick FM, Baya AM (1994) Comparison of the taxonomy, serology, drug resistance transfer, and virulence of Citrobacter freundii strains from mammals and poikilothermic hosts. Appl Environ Microbiol 60:1789–1797