Fastin situ determination of the biomass of anchorage-dependent cells
Tóm tắt
The lengthy and cumbersome protocol used to establish the growth kinetics characteristics of anchorage-dependent cells (ADC's)in situ (i.e. while the cells adhere on their microsupport) by Aperture Impedance Pulse Spectroscopy (AIPS) can be replaced by an accelerated procedure. This we have named Turbo AIPS whereby the same results can be obtained without actually performing the manipulations leading to the determination of the biomass.
Tài liệu tham khảo
Degouys V, Cerckel I, Garcia A, Harfield J, Dubois D, Fabry L and Miller AOA (1993) Dielectric spectroscopy of mammalian cells. 2. Stimultaneousin situ evaluation by aperture impedance pulse spectroscopy and low frequency dielectric spectroscopy of the biomass of HTC cells on Cytodex 3. Cytotechnology 13: 195–202.
Degouys V, Menozzi FD, Dubois D and Miller AOA (1995) Electronic sizing of microbeads covered with cells:in situ biomass evaluation by aperture impedance puise spectroscopy. In: Cell and Tissue Culture: Laboratory Procedures. Doyle A, Griffith JB and Newell DG (eds.)., John Wiley & Sons, Chichester. 28C∶3, 1–8.
Miller SJO, Henrotte M and Miller AOA (1986) Growth of animal cells on microbeads. IIn situ estimation of numbers. Biotechnol. and Bioengng 28: 1466–1473.
Thebline D, Harfield J, Hanotte O, Dubois D and Miller AOA (1987) Growth of animal cells on microbeads. III Semi continuousin situ estimation of numbers. p. 504–512. In: Modern approaches to animal cell technology. Spier RE and Griffiths JB (eds.). Butterworth & Co.