Fastin situ determination of the biomass of anchorage-dependent cells

Cytotechnology - Tập 19 - Trang 107-110 - 1995
V. Degouys1, J. Harfield2, L. Fabry3, F. D. Menozzi4, A. O. A. Miller1
1Université de Mons-Hainaut, MONS, Belgium
2Coulter Electronics Ltd, Luton, UK
3SmithKline Beecham Biologicals, Rixensart, Belgium
4Laboratoire de Microbiologie Génétique et Moléculaire, Institut Pasteur de Lille, Lille, France

Tóm tắt

The lengthy and cumbersome protocol used to establish the growth kinetics characteristics of anchorage-dependent cells (ADC's)in situ (i.e. while the cells adhere on their microsupport) by Aperture Impedance Pulse Spectroscopy (AIPS) can be replaced by an accelerated procedure. This we have named Turbo AIPS whereby the same results can be obtained without actually performing the manipulations leading to the determination of the biomass.

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