Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
Tóm tắt
Từ khóa
Tài liệu tham khảo
Calvez, 2020, Molecular detection of SARS-CoV-2 using a reagent-free approach, MedRxiv
Corman, 2020, Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR, Euro Surveill, 25
van Doremalen, 2020, Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1, N Engl J Med., 382, 1564, 10.1056/NEJMc2004973
Esbin, 2020, Overcoming the bottleneck to widespread testing: A rapid review of nucleic acid testing approaches for COVID-19 detection, RNA.
Fomsgaard, 2020, An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit shortage, Copenhagen, Denmark, March 2020, Euro Surveill, 25
Geller, 2012, Human Coronaviruses: Insights into Environmental Resistance and Its Influence on the Development of New Antiseptic Strategies, Viruses., 4, 3044, 10.3390/v4113044
Pastorino B., Touret F., Gilles M., de Lamballerie X., Charrel R.N. Evaluation of heating and chemical protocols for inactivating SARS-CoV-2. bioRxiv Apr; 10.1101/2020.04.11.036855.
Shedlovskiy, 2017, One-step hot formamide extraction of RNA from Saccharomyces cerevisiae, RNA Biol., 14, 1722, 10.1080/15476286.2017.1345417
Stead, 2012, RNA snap TM: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria, Nucleic Acids Res., 40
Xie, 2020, Chest CT for Typical 2019-nCoV Pneumonia: Relationship to Negative RT-PCR Testing, Radiology