Factors influencing circulating microRNAs as biomarkers for liver diseases

Springer Science and Business Media LLC - Tập 49 - Trang 4999-5016 - 2022
Shalini R. Dubey1, Tester F. Ashavaid2, Philip Abraham1, Minal Umesh Paradkar2
1Division of Gastroenterology, P. D. Hinduja Hospital & MRC, Mumbai, India
2Department of Biochemistry and Research, P. D. Hinduja Hospital & MRC, Mumbai, India

Tóm tắt

MicroRNAs (microRNAs) have been implicated to play crucial roles in various liver diseases. Hepatic microRNAs are released in to the circulation in a systematic fashion, and are, therefore, being extensively explored for their role as prognostic or diagnostic markers or therapeutic targets for numerous hepatic diseases. Advantages such as disease- or tissue-specific expression, and ease of detection have implicated circulating microRNAs (c-microRNAs) as the most desirable candidate for biomarker studies. Although several studies have explored c-microRNAs in serum or plasma, consistency of detection of microRNAs remains demanding because of many biological and methodological challenges. Lack of methodological consensus has limited the universal use of c-microRNAs as potential prognostic or diagnostic disease-specific biomarkers. In this review, we have discussed pre-analytical and analytical factors that might affect c-miRNA expression and selection of appropriate data normalization strategy by incorporating endogenous reference microRNAs. This review will aid designing of better approaches and protocols for future diagnostic research on hepatic c-microRNAs.

Tài liệu tham khảo

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