Facile 3D cell culture protocol based on photocurable hydrogels

Zhu Z, Liu J-D, Liu C, Wu X, Li Q, Chen S, Zhao X, Weitz DA (2020) Microfluidics-assisted assembly of injectable photonic hydrogels toward reflective cooling. Small 16(9):1903939

Jensen C, Teng Y (2020) Is it time to start transitioning from 2D to 3D cell culture? Front Mol Biosci 7:33

De Leon SE, Pupovac A, McArthur SL (2020) Three-dimensional (3D) cell culture monitoring: opportunities and challenges for impedance spectroscopy. Biotechnol Bioeng 117(4):1230–1240

Zhao X, Liu S, Yildirimer L, Zhao H, Ding R, Wang H, Cui W, Weitz D (2016) Injectable stem cell-laden photocrosslinkable microspheres fabricated using microfluidics for rapid generation of osteogenic tissue constructs. Adv FuncT Mater 26(17):2809–2819

Xie M, Gao Q, Zhao H, Nie J, Fu Z, Wang H, Chen L, Shao L, Fu J, Chen Z, He Y (2019) Electro-assisted bioprinting of low-concentration GelMA microdroplets. Small 15(4):1804216

Xie M, Gao Q, Qiu J, Fu J, Chen Z, He Y (2020) 3D biofabrication of microfiber-laden minispheroids: a facile 3D cell co-culturing system. Biomater Sci 8(1):109–117

Xie M, Gao Q, Fu J, Chen Z, He Y (2020) Bioprinting of novel 3D tumor array chip for drug screening. Bio-Des Manuf 3(3):175–188

Shao L, Gao Q, Xie C, Fu J, Xiang M, Liu Z, Xiang L, He Y (2020) Sacrificial microgel-laden bioink-enabled 3D bioprinting of mesoscale pore networks. Bio-Des Manuf 3(1):30–39

Nie J, Gao Q, Wang Y, Zeng J, Zhao H, Sun Y, Shen J, Ramezani H, Fu Z, Liu Z, Xiang M, Fu J, Zhao P, Chen W, He Y (2018) Vessel-on-a-chip with hydrogel-based microfluidics. Small 14(45):1802368

Xie M, Yu K, Sun Y, Shao L, Nie J, Gao Q, Qiu J, Fu J, Chen Z, He Y (2019) Protocols of 3D bioprinting of gelatin methacryloyl hydrogel based bioinks. JoVE 154:e60545

Yue K, Trujillo-de Santiago G, Alvarez MM, Tamayol A, Annabi N, Khademhosseini A (2015) Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 73:254–271

Yoon HJ, Shin SR, Cha JM, Lee S-H, Kim J-H, Do JT, Song H, Bae H (2016) Cold water fish gelatin methacryloyl hydrogel for tissue engineering application. PLoS ONE 11(10):e0163902

Yuan S, Kang Y, Jing N, Miao S, Jianzhong F, Huiming W, Yong H (2020) Modeling the printability of photocuring and strength adjustable hydrogel bioink during projection based 3D bioprinting. Biofabrication. https://doi.org/10.1088/1758-5090/aba413

Zhao X, Lang Q, Yildirimer L, Lin ZY, Cui W, Annabi N, Ng KW, Dokmeci MR, Ghaemmaghami AM, Khademhosseini A (2016) Photocrosslinkable gelatin hydrogel for epidermal tissue engineering. Adv Healthc Mater 5(1):108–118

Sun X, Lang Q, Zhang H, Cheng L, Zhang Y, Pan G, Zhao X, Yang H, Zhang Y, Santos HA, Cui W (2017) Electrospun photocrosslinkable hydrogel fibrous scaffolds for rapid in vivo vascularized skin flap regeneration. Adv Funct Mater 27(2):1604617

Chen H, Guo L, Wicks J, Ling C, Zhao X, Yan Y, Qi J, Cui W, Deng L (2016) Quickly promoting angiogenesis by using a DFO-loaded photo-crosslinked gelatin hydrogel for diabetic skin regeneration. J Mater Chem B 4(21):3770–3781

Prestwich GD (2007) Simplifying the extracellular matrix for 3-D cell culture and tissue engineering: a pragmatic approach. J Cell Biochem 101(6):1370–1383