Fabrication of hierarchical polycaprolactone/gel scaffolds via combined 3D bioprinting and electrospinning for tissue engineering

Springer Science and Business Media LLC - 2014
Yong-Ze Yu1, Lu-Lu Zheng1, Hai-Ping Chen1, Wei-Hua Chen1, Qing-Xi Hu1
1Rapid Manufacturing Engineering Center, Shanghai University, Shanghai, People’s Republic of China

Tóm tắt

It is a severe challenge to construct 3D scaffolds which hold controllable pore structure and similar morphology of the natural extracellular matrix (ECM). In this study, a compound technology is proposed by combining the 3D bioprinting and electrospinning process to fabricate 3D scaffolds, which are composed by orthogonal array gel microfibers in a grid-like arrangement and intercalated by a nonwoven structure with randomly distributed polycaprolactone (PCL) nanofibers. Human adipose-derived stem cells (hASCs) are seeded on the hierarchical scaffold and cultured 21 d for in vitro study. The results of cells culturing show that the microfibers structure with controlled pores can allow the easy entrance of cells and the efficient diffusion of nutrients, and the nanofiber webs layered in the scaffold can significantly improve initial cell attachment and proliferation. The present work demonstrates that the hierarchical PCL/gel scaffolds consisting of controllable 3D architecture with interconnected pores and biomimetic nanofiber structures resembling the ECM can be designed and fabricated by the combination of 3D bioprinting and electrospinning to improve biological performance in tissue engineering applications.

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Tài liệu tham khảo

Vacanti JP, Morse MA, Saltzman WM (1988) Selective cell transplantation using bioabsorbable artificial polymers as matrices. J Pediatr Surg 23:3–9

Langer R, Vacanti JP (1993) Tissue engineering. Science 260:920–926

Wang DL (2011) Stem cells tissue engineering technology. Sci. Pr., Beijing

Wang SG (2001) Cells scaffold of tissue engineering and the related technologies. Modern Rehabil. 5(16–17):41

Shuai CJ, Mao ZZ, Lu HB, Nie Y, Hu HL, Peng SP (2013) Fabrication of porous polyvinyl alcohol scaffold for bone tissue engineering via selective laser sintering. Biofabrication 5:015014

Poot AA, Grijpma DW, Feijen J, Vermes I (2012) Vascular tissue engineering. Ned Tijdschr Klin Chem Labgeneesk 37:44–46

Carfi PF, Rigogliuso S, La Carrubba V, Mannella GA, Ghersi G, Brucato V (2012) Poly lactic acid based scaffolds for vascular tissue engineering. Chem Eng Trans 27:409–414

Teixeira AI, Abrams GA, Bertics PJ, Murphy CJ, Nealey PF (2003) Epithelial contact guidance on well-defined micro-and nanostructured substrates. J Cell Sci 116:1881–1892

Park SA, Kim HJ, Lee SH, Lee JH, Kim HK, Yoon TR (2011) Fabrication of nano/microfiber scaffolds using a combination of rapid prototyping and electrospinning systems. Polym Eng Sci 51:1883–1890

Gautam S, Dinda AK, Mishra NC (2013) Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method. Mater Sci Eng 33:1228–1235

Duan B, Cheung WL, Wang M (2011) Optimized fabrication of Ca-P/PHBV nanocomposite scaffolds via selective laser sintering for bone tissue engineering. Biofabrication 3:015001

Melchels FPW, Domingos MAN, Klein TJ, Malda J, Bartolo PJ, Hutmacher DW (2012) Additive manufacturing of tissues and organs. Prog Polym Sci 37:1079–1104

Nair K, Gandhi M, Khalil S, Yan KC, Marcolongo M, Barbee K, Sun W (2009) Characterization of cell viability during bioprinting processes. Biotechnol J 4:1168–1177

Seyednejad H, Gawlitta D, Kuiper RV, Bruin AD, Nostrum CF, Vermonden T, Dhert WJA, Hennink WE (2012) In vivo biocompatibility and biodegradation adation of 3D-printed porous scaffolds based on a hydroxyl-functionalized poly (ε-caprolactone). Biomaterials 33:4309–4318

Dombrowski F, Caso PWG, Laschke MW, Klein M, Gunster J, Berger G (2013) 3-D printed bioactive bone replacement scaffolds of alkaline substituted ortho-phosphates containing meta- and di-phosphates. Key Eng Mater 529–530:138–142

Ibrahim TO, Yin Y (2013) Bioprinting toward organ fabrication: challenges and future trends. IEEE Trans Biomed Eng 60:691–699

Liu HX, Yan YN, Wang XH, Xiong Z, Cheng J (2005) Three-dimensional controlled assembling of hepatocytes. J Tsinghua Univ (Sci & Tech) 45: 1009–1011, 1031

Landers R, Hubnerb U, Schmelzeisenb R (2002) Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. Biomaterials 23:4437–4447

Liu F, Zhang RJ, Yan YN, Liu HX (2006) Rapid prototyping of gelatin/sodium alginate tissue engineering scaffolds. J Tsinghua Univ (Sci & Tech) 46:1357–1360

Eladale T, Bard J (1972) Collagen substrata for studies on cell behavior. J Cell Biol 54:626–637

Colon G, Ward BC, Matster TJ (2006) Increased osteoblast and decreased staphylococcus epidermis functions on nanophase ZnO and TiO2. J Biomed Mater Res A 78:595–604

Ye L, Zeng XC, Li HJ, Ai Y (2010) Fabrication and biocompatibility of nano non-stoichiometric apatite and poly (epsilon-caprolactone) composite scaffold by using prototyping controlled process. J Mater Sci 21:753–760

Baker BM, Handorf AM, Ionescu LC, Li WJ, Mauck RL (2009) New directions in nanofibrous scaffolds for soft tissue engineering and regeneration. Expert Rev Med Devices 6:515–532

Xu H, Cui WG, Chang J (2013) Fabrication of patterned PDLLA/PCL composite scaffold by electrospinning. J Appl Polym Sci 127:1550–1554

Kim GM, Le KHT, Giannitelli SM, Lee YJ, Rainer A, Trombetta M (2013) Electrospinning of PCL/PVP blends for tissue engineering scaffolds. J Mater Sci 24:1425–1442

Liu DL, Liu YY, Wang QG, Jing CJ, Hu QX (2012) Multiphysics coupling analysis and experiment of low-temperature deposition manufacturing and electrospinning for multi-scale tissue engineering scaffold. J Mech Eng 48:137–143

Ramay HR, Zhang M (2003) Preparation of porous hydroxyapatite scaffolds by combination of the gel-casting and polymer sponge methods. Biomaterials 24:3293–3302

Mikos AG, Thorsen AJ, Czerwonka LA (1994) Preparation and characterization of poly (l-lactic acid) foams. Polymer 35:1068–1077

Stoppato M, Stevens HY, Carletti E, Migliaresi C, Motta A, Guldberg RE (2013) Effects of silk fibroin fiber incorporation on mechanical properties, endothelial cell colonization and vascularization of PDLLA scaffolds. Biomaterials 34:4573–4581

Matsuzaka K, Walboomers XF, Ruijter JE (1999) The effects of poly-lactic acid with parallel surface micro groove on osteoblast growth factor. Biomaterials 20:1293–1301

Nam YS, Yoon JJ, Park TG (2000) A novel fabrication method of macroporous biodegradable polymer scaffolds using gas forming salt as a porogen additive. J. Biomed Mater Res Part B 53:1–7

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