FMRFamide-related peptides inHymenolepis diminuta: Immunohistochemistry and radioimmunoassay
Tóm tắt
The localization of FMRFamide-related peptide (FaRP) immunoreactivity was determined during different stages of development of the rat tapewormHymenolepis diminuta. In the adult worm (14 days old), FaRP immunostaining was most intense in the scolex and concentrated in the central nervous system (cerebral ganglia and transverse commissures) and around the lips of the suckers. In the strobila, medial and lateral longitudinal nerve cords (LNCs) and ladder-like connecting commissures were the only tissue stained. Immunoreactivity in the medial LNCs of the adult tapeworms extended only to and included proglottides containing developing testis and seminal receptacle but disappeared in proglottides in which primordial ovaries were first detected. Radioimmunoassay confirmed that FaRPs were concentrated in the scolex/neck region of the adult worm (3.9±1.5 pmol mg protein−1), whereas the lowest concentrations (0.2±0.19 pmol mg protein−1) were recovered from the regions of the strobila containing shelled eggs. The pattern of FaRP immunoreactivity observed in 5- and 7-day-old worms was similar to that seen in adult worms, but in 2- and 3-day-old worms the pattern of immunoreactivity observed in the cerebral ganglia, transverse commissures, and LNCs differed significantly as compared with that seen in older worms. These results indicate differential utilization and/or roles for FaRPs during development and suggest both central and sensory roles in this tapeworm.
Tài liệu tham khảo
Braten T, Hopkins CA (1969) The migration ofHymenolepis diminuta in the rat's intestine during normal development and following surgical transplantation. Parasitology 59:891–905
Chalfie M (1984) Neuronal development ofCaenorhabditis elegans. Trends Neurosci 7:197–202
Cottrell GA (1989) The biology of the FMRFamide-series of peptides in molluscs with special reference toHelix. Comp Biochem Physiol [A] 93:41–45
Fairweather I, Halton DW (1991) Neuropeptides in platyhelminths. Parasitology 102:S77-S92
Fairweather I, Macartney GA, Johnston CF, Halton DW, Buchanan KD (1988) Immunocytochemical demonstration of 5-hydroxytryptamine (serotonin) and vertebrate neuropeptides in the nervous system of excysted cysticercoid larvae of the rat tapeworm,Hymenolepis diminuta (Cestoda, Cyclophyllidea). Parasitol Res 74:371–379
Fairweather I, Mahendrasingam S, Johnston CF, Halton DW, Shaw C (1990) Peptidergic nerve elements in three developmental stages of the tetraphyllidean tapewormTrilocularia acanthiaevulgaris. An immunocytochemical study. Parasitol Res 76:497–508
Goodchild CG, Harrison DL (1961) The growth of the rat tapeworm,Hymenolepis diminuta, during the first five days in the final host. J Parasitol 47:819–829
Goodman C, Spitzer N (1978) Embryonic development of identified neurones: differentiation from neuroblast to neurone. Nature 280:208–214
Greenberg MJ, Payza K, Nachman RJ, Holman GM, Price DA (1988) Relationships between the FMRFamide-related peptides and other peptide families. Peptides. 9 [Suppl 1]: 125–135
Gupta BC, Basch PF (1989) Human chorionic gonadotropin-like immunoreactivity in schistosomes andFasciola. Parasitol Res 76:86–89
Gustafsson MKS (1991) Skin the tapeworms before you stain their nervous system! A new method for whole-mount immunocytochemistry. Parasitol Res 77:509–516
Gustafsson MKC, Wikgren MC (1981) Activation of the peptidergic neurosecretory system inDiphyllobothrium dendriticum (Cestoda: Pseudophyllidea). Parasitology 83:243–247
Gustafsson MKS, Jukanen AC, Wikgren MC (1983) Activation of the peptidergic neurosecretory system inDiphyllobothrium dendriticum (Cestoda) at suboptimal temperatures. Z Parasitenkd 69:279–282
Gustafsson MKS, Wikgren MC, Karhi TJ, Schot LPC (1985) Immunocytochemical demonstration of neuropeptides and serotonin in the tapewormDiphyllobothrium dendriticum. Cell Tissue Res 240:255–260
Halton DW, Shaw C, Maule AG, Johnston CF, Fairweather I (1992) peptidergic messengers: a new perspective of the nervous system of parasitic platyhelminths. J Parasitol 78:179–193
Hauser M, Koopowitz H (1987) Age-dependent changes in fluorescent neurons in the brain ofNotoplana acticola, a polyclad flatworm. J Exp Zool 241:217–225
Landis SC, Keefe D (1983) Evidence for neurotransmitter plasticity in vivo: developmental changes in properties of cholinergic sympathetic neurons. Dev Biol 98:349–372
Lange AB, Orchard I, The Brugge VA (1991) Evidence for the involvement of a SchistoFLRF-amide-like-peptide in the neural control of locust oviduct. J Comp Physiol [A] 168:383–391
Levine RB (1984) Changes in neuronal circuits during insect metamorphosis. J Exp Biol 112:27–44
Lumsden RD, Specian R (1980) The morphology, histology, and fine structure of the adult stage of the cyclophyllidean tapewormHymenolepis diminuta. In: Arai HP (ed) Biology of the tapewormHymenolepis diminuta. Academic Press, New York, pp 157–280
Magee RM, Fairweather I, Johnston CF, Halton DW, Shaw C (1989) Immunocytochemical demonstration of neuropeptides in the nervous system of the liver fluke,Fasciola hepatica (Trematoda, Digenea). Parasitology 98:227–238
Maule Ag, Halton DW, Johnston CF, Shaw C, Fairweather I (1990) The serotoninergic, cholinergic and peptidergic components of the nervous system in the monogenean parasite,Diclidophora merlangi: a cytochemical study. Parasitology 100:255–273
Maule AG, Shaw C, Halton DW, Thim L, Johnston CF, Fairweather I, Buchanan KD (1991) Neuropeptide F: a novel parasitic flatworm regulatory peptide fromMoniezia expansa (Cestoda: Cyclophyllidea). Parasitology 102:309–316
McFarlane ID, Graff D, Grimmelikhuijzen CJP (1987) Excitatory actions of Antho-RFamide, an anthozoan neuropeptide, on muscles and conducting systems in the sea anemoneCalliactis parasitica. J Exp Biol 133:157–168
McKay DM, Fairweather I, Johnston CF, Shaw C, Halton DW (1991a) Immunocytochemical and radioimmunometrical demonstration of serotonin- and neuropeptide-immunoreactivities in the adult rat tapeworm,Hymenolepis diminuta (Cestoda, Cyclophyllidea). Parasitology 103:275–289
McKay DM, Fairweather I, Johnston CF, Shaw C, Halton DW (1991b) Cytochemical demonstration of cholinergic, serotoninergic and peptidergic nerve elements inGorgoderina vitelliloba (Trematoda: Digenea). Int J Parasitol 21:71–80
Price DA, Greenberg MJ (1977) Structure of a molluscan cardioexcitatory neuropeptide. Science 197:670–671
Price DA, Greenberg MJ (1989) The hunting of the FMRFamidelikes: the distribution of FMRFamide-related peptides. Biol Bull 177:198–205
Read CP, Kilejian AZ (1969) Circadian migratory behavior of a cestode symbiote in the rat host. J Parasitol 55:574–578
Roberts LS (1961) The influence of population density on patterns and physiology of growth inHymenolepis diminuta (Cestoda: Cyclophyllidea) in the definitive host. Exp Parasitol 11:332–371
Roberts LS (1980) Development ofHymenolepis diminuta in its definitive host. In: Arai HP (ed) Biology of the tapewormHymenolepis diminuta. Academic Press, New York, pp 357–371
Schinkmann K, Li C (1992) Localization of FMRFamide-like peptides inCaenorhabditis elegans. J Comp Neurol 316:251–260
Schneider LE, O'Brien MA, Taghert PH (1991) In situ hybridization analysis of the FMRFamide neuropeptide gene inDrosphila. I. Restricted expression in embryonic and larval stages. J Comp Neurol 304:608–622
Specian RD, Lumsden RD, Ubelaker JE, Allison VF (1979) A unicellular endocrine gland in cestodes. J Parasitol 65:569–578
Sukhdeo MVK, Mettrick DF (1987) Parasite behaviour: understanding platyhelminth responses. Adv Parasitol 26:73–144
Webb RA (1977) Evidence for neurosecretory cells in the cestodeHymenolepis microstoma. Can J Zool 55:1726–1733
Webb RA (1988) Endocrinology of acoelomates. In: Downer RGH, Laufer H (eds) Invertebrate endocrinology, vol 2. Endocrinology of selected invertebrate types. Alan R. Liss, New York, pp 31–62
Wilson VCLC, Schiller EL (1969) The neuroanatomy ofHymenolepis diminuta andH. nana. J Parasitol 55:261–270