Extrahepatic and Intrahepatic Human Portal Interstitial Cajal Cells

Anatomical Record - Tập 294 Số 8 - Trang 1382-1392 - 2011
Mugurel Constantin Rusu1,2, F. Pop3, Sorin Hostiuc4, George Cristian Curcă5, Adrian Streinu‐Cercel6,7
1Department of Plant and Animal Cytobiology, Institute of Biology – Romanian Academy, Bucharest, Romania
2Discipline of Anatomy, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
3Discipline of Pathologic Anatomy, Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
4“Mina Minovici” National Institute of Legal Medicine, Bucharest, Romania
5Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
6Discipline of Infectious Diseases, Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
7“Prof. Dr. Matei Balş” National Institute for Infectious Diseases, Bucharest, Romania

Tóm tắt

AbstractPortal interstitial cells of Cajal (PICCs), acting as vascular pacemakers, were previously only identified in nonhumans. Moreover, there is no evidence available about the presence of such cells within the liver. The objective of the study is to evaluate whether or not PICCs are identifiable in humans and, if they are, whether or not they are following the scaffold of portal vein (PV) branches within the liver. We obtained extrahepatic PVs and liver samples from six adult human cadavers, negative for liver disease, in accordance with ethical rules. They were stained with hematoxylin‐eosin (HE) and Giemsa, and then we performed immunohistochemistry on formalin‐fixed paraffin‐embedded specimens for CD117/c‐kit, a marker of the Cajal's cells. Immune labeling was also performed for S‐100 protein, desmin, glial fibrillary acidic protein (GFAP), neurofilaments, α‐smooth muscle actin (α‐SMA), and CD34. c‐kit‐Positive PICCs were identified within the extrahepatic PV, in portal spaces, and septa. On adjacent sections, these PICCs were negative for all the other antibodies used. In conclusion, our study confirms the presence of extrahepatic PICCs on humans, which may act as a possible intrinsic pacemaker in the human PV. However, the intrahepatic PICCs, which were evidenced here for the first time, are in need for further experimental studies to evaluate their functional role. A promising further direction of the study is the PICCs role in the idiopathic portal hypertension. Anat Rec, 2011. © 2011 Wiley‐Liss, Inc.

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Tài liệu tham khảo

10.1111/j.1440-1746.2009.05980.x

10.1016/j.ceca.2004.01.018

10.1111/j.1768-322X.1996.tb00967.x

10.1152/ajpcell.00147.2006

10.1152/ajpgi.2001.281.6.G1329

10.1002/ar.20715

10.1111/j.1582-4934.2010.01074.x

10.1007/s00441-010-1114-1

10.1016/S0165-5728(85)80073-4

10.1111/j.1582-4934.2008.00444.x

10.1007/s10585-008-9191-1

10.1161/01.RES.0000143014.04535.a3

10.1111/j.1582-4934.2005.tb00352.x

10.1152/ajpheart.00002.2004

10.1007/s10735-007-9099-0

10.1111/j.1582-4934.2006.tb00306.x

10.1111/j.1582-4934.2005.tb00394.x

Huang X, 2010, The pacemaker functions of visceral interstitial cells of Cajal, Sheng Li Xue Bao, 62, 387

10.1111/j.1582-4934.2005.tb00372.x

10.1053/j.gastro.2009.09.023

10.2353/ajpath.2009.081061

10.1113/jphysiol.2006.116855

10.1113/jphysiol.2006.122861

Leong ASY, 2003, Manual of diagnostic antibodies for immunohistology

10.1007/s00428-009-0739-5

10.1016/j.hrthm.2008.03.057

10.1055/s-2002-23207

10.1111/j.1582-4934.2005.tb00376.x

10.1111/j.1582-4934.2010.01059.x

10.1111/j.1582-4934.2006.tb00410.x

10.1111/j.1582-4934.2005.tb00347.x

10.1016/S0143-4160(02)00197-5

10.1100/tsw.2010.123

10.1369/jhc.4A6514.2005

10.1111/j.1582-4934.2005.tb00509.x

10.1016/S0074-7696(03)29004-5

10.1146/annurev.physiol.68.040504.094718

Sanders KM, 2000, A novel pacemaker mechanism drives gastrointestinal rhythmicity, News Physiol Sci, 15, 291

10.1078/0344-0338-00308

10.1152/ajpgi.2001.281.3.G602

10.1002/hep.1840040425

10.1007/s10735-007-9078-5

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Anatomical Record

Tập 294 Số 8

1382-1392

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