Extracts of medicinal plants with natural deep eutectic solvents: enhanced antimicrobial activity and low genotoxicity

Springer Science and Business Media LLC - 2020
Tsvetinka Grozdanova1, Boryana Trusheva1, Kalina Alipieva1, Milena Popova1, Lyudmila Dimitrova2, Hristo Najdenski2, Maya M. Zaharieva2, Yana Ilieva2, Bela Vasileva3, George Miloshev3, Milena Georgieva3, Vassya Bankova1
1Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str, BL. 9, 1113 Sofia, Bulgaria
2The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 26, 1113, Sofia, Bulgaria
3Institute of Molecular Biology “Roumen Tsanev”, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113, Sofia, Bulgaria

Tóm tắt

Abstract

Natural deep eutectic solvents (NADES) are a new alternative to toxic organic solvents. Their constituents are primary metabolites, non-toxic, biocompatible and sustainable. In this study four selected NADES were applied for the extraction of two medicinal plants: Sideritis scardica, and Plantago major as an alternative to water-alcohol mixtures, and the antimicrobial and genotoxic potential of the extracts were studied. The extraction efficiency was evaluated by measuring the extracted total phenolics, and total flavonoids. Best extraction results for total phenolics for the studied plants were obtained with choline chloride-glucose 5:2 plus 30% water; but surprisingly these extracts were inactive against all tested microorganisms. Extracts with citric acid-1,2-propanediol 1:4 and choline chloride-glycerol 1:2 showed good activity against S. pyogenes, E. coli, S. aureus, and C. albicans. Low genotoxicity and cytotoxicity were observed for all four NADES and the extracts with antimicrobial activity. Our results confirm the potential of NADESs for extraction of bioactive constituents of medicinal plants and further suggest that NADES can improve the effects of bioactive extracts. Further studies are needed to clarify the influence of the studied NADES on the bioactivity of dissolved substances, and the possibility to use such extracts in the pharmaceutical and food industry.

Từ khóa


Tài liệu tham khảo

Dai Y, van Spronsen J, Witkamp GJ, Verpoorte R, Choi YH (2013) Natural deep eutectic solvents as new potential media for green technology. Anal Chim Acta 766:61–68

Jeong KM, Ko J, Zhao J, Jin Y, Han SY, Lee J (2017) Multi-functioning deep eutectic solvents as extraction and storage media for bioactive natural products that are readily applicable to cosmetic products. J Clean Prod 151:87–95

Ruesgas-Ramón M, Figueroa-Espinoza MC, Durand E (2017) Application of deep eutectic solvents (DES) for phenolic compounds extraction: overview, challenges, and opportunities. J Agric Food Chem 65(18):3591–3601

Ogihara W, Aoyama T, Ohno H (2004) Polarity measurement for ionic liquids containing dissociable protons. Chem Lett 33(11):1414–1415

Espino M, de los Ángeles Fernández M, Gomez FJ, Silva MF (2016) Natural designer solvents for greening analytical chemistry. TrAC Trends Analyt Chem 76:126–136

Dimitrova L, Zaharieva MM, Popova M, Kostadinova N, Tsvetkova I, Bankova V, Najdenski H (2017) Antimicrobial and antioxidant potential of different solvent extracts of the medicinal plant Geum urbanum L. Chem Cent J 11:113

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63

ISO 10993-5:2009 (2017) Biological evaluation of medical devices—Part 5: tests for in vitro cytotoxicity, in ICS 11.100.20. International Organization for Standardization. https://www.iso.org/standard/36406.html.

Dai Y, Witkamp GJ, Verpoorte R, Choi YH (2015) Tailoring properties of natural deep eutectic solvents with water to facilitate their applications. Food chem 187:14–19

Todorova M, Trendafilova A (2014) Sideritis scardica Griseb., an endemic species of Balkan peninsula: traditional uses, cultivation, chemical composition, biological activity. J Ethnopharmacol 152(2):256–265

Samuelsen AB (2000) The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J Ethnopharmacol 71(1–2):1–21

Murador DC, de Souza Mesquita LM, Vannuchi N, Braga ARC, de Rosso VV (2019) Bioavailability and biological effects of bioactive compounds extracted with natural deep eutectic solvents and ionic liquids: advantages over conventional organic solvents. Cur Opin Food Sci 26:25–34

Wikene KO, Rukke HV, Bruzell E, Tønnesen HH (2017) Investigation of the antimicrobial effect of natural deep eutectic solvents (NADES) as solvents in antimicrobial photodynamic therapy. J Photochem Photobiol B 171:27–33

Trusheva B, Petkov H, Popova M, Dimitrova L, Zaharieva M, Tsvetkova I, Najdenski H, Bankova V (2019) “Green” approach to propolis extraction: natural deep eutectic solvents. C R Acad Bulg 72(7):897–905

Gutiérrez MC, Ferrer ML, Mateo CR, del Monte F (2009) Freeze-drying of aqueous solutions of deep eutectic solvents: a suitable approach to deep eutectic suspensions of self-assembled structures. Langmuir 25(10):5509–5515

Radošević K, Čanak I, Panić M, Markov K, Bubalo MC, Frece J, Srček VG, Redovniković IR (2018) Antimicrobial, cytotoxic and antioxidative evaluation of natural deep eutectic solvents. Environ Sci Pollut Res 25(14):14188–14196

Eliaz I, Weil E, Schwarzbach J, Wilk B (2019) Modified citrus pectin/alginate dietary supplement increased fecal excretion of ranium: a family. Altern Ther Health Med 25(4):20–24

Peycheva E, Alexandrova R, Miloshev G (2014) Application of the yeast comet assay in testing of food additives for genotoxicity. LWT Food Sci Technol 59(1):510–517

Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35(3):206–221

Bilal M, Iqbal HMN (2019) An insight into toxicity and human-health-related adverse consequences of cosmeceuticals—a review. Sci Total Environ 670:555–568

Draganova-Filipova M, Mourdjeva M, Popova Z, Peycheva E, Miloshev G, Sarafian V (2009) Propolis induced apoptosis in MC Coy-Plovdiv Cells. Biotechnol Biotechnol Equip 23(sup1):635–638

Bajpayee M, Kumar A, Dhawan A (2013) The comet assay: assessment of in vitro and in vivo DNA damage. In: Dhawan A, Bajpayee M (eds) Genotoxicity assessment. Humana, New York, pp 237–257

Collins AR (2015) The comet assay: a heavenly method! Mutagenesis 30(1):1–4

Staneva D, Peycheva E, Georgieva M, Efremov T, Miloshev G (2013) Application of comet assay for the assessment of DNA damage caused by chemical genotoxins in the dairy yeast Kluyveromyces lactis. Antonie Van Leeuwenhoek 103(1):143–152

Fairbairn DW, Olive PL, O’Neill KL (1995) The comet assay: a comprehensive review. Mutat Res 339(1):37–59

Peycheva E, Georgieva M, Miloshev G (2009) Comparison between alkaline and neutral variants of yeast comet assay. Biotechnol Biotechnol Equip 23(1):1090–1092

Thông tin
Thông tin xuất bản

Springer Science and Business Media LLC

Thông tin tác giả