Extraction, purification, characterization and antioxidant activities of polysaccharides from Ramaria botrytis (Pers.) Ricken
Tóm tắt
Ramaria botrytis (Pers.) Ricken, a member of the family Clavariaceae, has been widely prescribed for anti-aging and improving immunity. To extract and purify the polysaccharides, the main constituent of the fruiting-body, from R. botrytis and explore antioxidant activities was great significant.
Ramaria botrytis polysaccharides (RBP) was extracted with water at 88.47 °C for 1.42 h with a solution to sample ratio of 10.94 mL g−1 employing response surface methodology. Four purified fractions, RBP-1, RBP-2, RBP-3, and RBP-4, were obtained from column chromatography of DEAE-52 and Sephadex G-100. Among these four purified fractions, RBP-1, RBP-2, RBP-4 were mainly composed of glucose, while RBP-3 contained 41.36% mannose and 28.96% glucose. The molecular weights of RBP-1, RBP-2, RBP-3 and RBP-4 were 6.48, 36.12, 96.72 and 8.34 kDa, respectively. These four fractions are also tested for antioxidant activities in vitro, RBP-4 exhibited strong assay of reducing power and high scavenging activity on DPPH radical, while RBP-3 showed the stronger ability of hydroxyl radical scavenging activity. Response surface methodology was successfully applied to optimize the ultrasonic extraction of polysaccharides from R. botrytis. RBP is an efficient natural antioxidant.
Tài liệu tham khảo
Kalac P (2009) Chemical composition and nutritional value of European species of wild growing mushrooms: a review. Food Chem 113:9–16
Unekwu HR, Audu JA, Makun MH, Chidi EE (2014) Phytochemical screening and antioxidant activity of methanolic extract of selected wild edible Nigerian mushrooms. Asian Pac J Trop Dis 4:153–157
Moon SM, Kim JS, Kim HJ, Choi MS, Park BR, Kim SG, Ahn H, Chun HS, Shin YK, Kim JJ, Kim DK, Lee SY, Seo YW, Kim YH, Kim CS (2014) Purification and characterization of a novel fibrinolytic α chymotrypsin like serine metalloprotease from the edible mushroom, Lyophyllum shimeji. J Biosci Bioeng 117:544–550
Zhang YY, Li S, Wang XH, Zhang L, Cheung PCK (2011) Advances in lentinan: isolation, structure, chain conformation and bioactivities. Food Hydrocoll 25:196–206
Wang QJ, Fang YZ (2004) Analysis of sugars in traditional Chinese drugs. J Chromatogr B 812:309–324
Zhang M, Wang F, Liu R, Tang XL, Zhang Q, Zhang ZS (2014) Effects of superfine grinding on physicochemical and antioxidant properties of Lycium barbarum polysaccharides. LWT-Food Sci Technol 58:594–601
Xu HS, Wu YW, Xu SF, Sun HX, Chen FY, Yao L (2009) Antitumor and immunomodulatory activity of polysaccharides from the roots of Actinidia eriantha. J Ethnopharmacol 125:310–317
Zhang T, Tian Y, Jiang B, Miao M, Mu WM (2014) Purification, preliminary structural characterization and in vitro antioxidant activity of polysaccharides from Acanthus ilicifolius. LWT-Food Sci Technol 56:9–14
Chen TQ, Wu YB, Wu JG, Ma L, Dong ZH, Wu JZ (2014) Efficient extraction technology of antioxidant crude polysaccharides from Ganoderma lucidum (Lingzhi), ultrasonic-circulating extraction integrating with superfine- pulverization. J Taiwan Inst Chem E 45:57–62
Tang XH, Yan LF, Gao J, Yang XL, Xu YX, Ge HY, Yang HD (2012) Antitumor and immunomodulatory activity of polysaccharides from the root of Limonium sinense Kuntze. Int J Biol Macromol 51:1134–1139
Bhanja SK, Rout D, Patra P, Sen IK, Nandanet CK, Islam SI (2014) Water-insoluble glucans from the edible fungus Ramaria botrytis. Bioact Carbohyd Diet Fibre 3:52–58
Jiang GX, Wen LR, Chen F, Wu FW, Lin S, Yang B, Jiang YM (2013) Structural characteristics and antioxidant activities of polysaccharides from longan seed. Carbohyd Polym 92:758–764
Lu CH, Engelmann NJ, Lila MA, Erdman JW (2008) Optimization of lycopene extraction from tomato cell suspension culture by response surface. Agr Food Chem 56:7710–7714
You QH, Yin XL, Zhang SN, Jiang ZH (2014) Extraction, purification, and antioxidant activities of polysaccharides from Tricholoma mongolicum Imai. Carbohyd Polym 99:1–10
Dubois M, Gilles KA, Hamilton JK, Rebers P, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Qiao DL, Hu B, Gan D, Sun Y, Ye H, Zeng XX (2009) Extraction optimized by using response surface methodology, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii. Carbohyd Polym 76:422–429
Wu WL, Zhu YT, Zhang L, Yang RW, Zhou YH (2012) Extraction, preliminary structural characterization, and antioxidant activities of polysaccharides from Salvia miltiorrhiza Bunge. Carbohyd Polym 87:1348–1353
Oyaizu M (1986) Studies on products of browning reaction: antioxidant activities of products browning reaction prepared from glucosamine. Jpn J Nutr 44:307–313
Jia XJ, Ding CB, Yuan S, Zhang ZW, Chen YE, Du L, Yuan M (2014) Extraction, purification and characterization of polysaccharides from Hawk Tea. Carbohyd Polym 99:319–324
Meir S, Kanner J, Akiri B, Hadas SP (1995) Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 43:1813–1819
Chen GT, Ma XM, Liu ST, Liao YL, Zhao GQ (2012) Isolation, purification and antioxidant activities of polysaccharides from Grifola frondosa. Carbohyd Polym 89:61–66