Extraction of chlorophyll and carotenoid pigments from Antarctic benthic mats for analysis by HPLC
Tóm tắt
The efficiency of 9:1 acetone-water, DMSO and boiling 9:1 ethanol-water in extracting chlorophyll and carotenoid pigments from benthic cyanobacterial mats from Antarctica for HPLC (high performance liquid chromatography) analysis was examined. Considerable breakdown of chlorophylla was observed after 5 min extraction in boiling ethanol and 2 h extraction in DMSO. Over 50% of the chlorophylla was degraded to chlorophyllidea and there was substantial loss of carotenoids after a 15 h exposure of ground cells to cold 9:1 acetone-water. Mild sonication of ground mat material in 9:1 acetone-water followed by a 4 h extraction at 4 ° C was found to minimise chlorophylla breakdown and dramatically improved the extraction efficiency of chlorophylla, myxoxanthophyll and -carotene.
Tài liệu tham khảo
Howard-Williams C, Pridmore R, Downes MT, Vincent WF (1989) Microbial biomass, photosynthesis and chlorophylla related pigments in the ponds of the McMurdo Ice Shelf, Antarctica. Ant. Sci. 1: 125–131.
Lock MA (1981) River epilithon — a light and organic energy transducer. In Lock MA, Williams DD (eds), Perspectives in Running Water Ecology, Plenum Press, New York, 3–40.
Mantoura RFC, Llewellyn CA (1983) The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography. Analyt. chim. Acta 151: 297–314.
Paerl HW, Lewin RA, Cheng L (1984) Variations in chlorophyll and carotenoid pigmentation amongProchloron (Prochlorophyta) symbionts in diverse marine ascidians. Bot. mar. 27: 257–264.
Sartory DP, Grobbelaar JU (1984) Extraction of chlorophylla from freshwater phytoplankton for spectrophotometric analysis. Hydrobiologia 114: 177–187.
Stauber JL, Jeffrey SW (1988) Photosynthetic pigments in fifty-one species of marine diatoms. J. Phycol. 24: 158–172.
Wright SW, Shearer JD (1984) Rapid extraction and highperformance liquid chromatography of chlorophylls and carotenoids from marine phytoplankton. J. Chromatogr. 294: 281–295.