Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis

Nascimento W C A, Martins M L L. Production and properties of an extracellular protease from thermophilic Bacillus sp. Braz J Microbiol, 2004, 35: 1–2

Pastor M D, Lorda G S, Balatti A. Protease obtention using Bacillus subtilis 3411 and amaranth seed meal medium at different aeration rates. Braz J Microbiol, 2001, 32: 1–8

Ward O P. Proteolytic Enzymes. Oxford: Pergamon Press, 1985, 789–818

Sookkheo B, Sinchaikul S, Phutrakul S, et al. Purification and characterization of the highly thermostable proteases from Bacillus stearothermophilus TLS33. Protein Expr Purif, 2000, 20: 142–151

Oledzka G., Dabrowski S, Kur J. High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris. Protein Expr Purif, 2003, 29: 223–229

Ferrero M A, Castro G R, Abate C M, et al. Thermostable alkaline protease of Bacillus licheniformis MIR29: isolation, production and characterization. Appl Microbiol Biotechnol, 1996, 45: 327–332

Kumar C G. Purification and characterization of a thermostable alkaline protease from alkalophilic Bacillus pumilus. Lett Appl Microbiol, 2002, 34: 13–17

Hyenung J J, Byoung C K, Yu R P, et al. A novel subtilisn-like serine protease from Thermoanerobacter yonseiensis KB-1: Its cloning, expression, and biochemical properties. Extremophiles, 2002, 6: 233–243

Helmann J D. Compilation and analysis of Bacillus subtilis σA-dependent promoter sequences: Evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucleic Acids Res, 1995, 23: 2351–2360

Van den Burg B, Eijsink V G H, Veltman O R, et al. Evolution reversed: Engineering an enzyme to resist boiling. Proc Natl Acad Sci USA, 1998, 95: 2056–2060

Rao M B, Tankasale A M, Ghatge M S, et al. Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev, 1998, 62: 597–634

Fujii M, Takagi M, Imanaka T, et al. Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis. J Bacteriol, 1983, 154: 831–837

Huang G R, Ying T J, Huo P, et al. Purification and characterization of a protease from Thermophilic bacillus strain HS08. Afr J Biotechnol, 2006, 5(24): 2433–2438

Doi R H, Wong S L, Kawamura F. Potential use of Bacillus subtilis for secretion and production of foreign proteins. Trends Biotechnol, 1986, 4: 232–235

Goeddle D V. Expression in B. subtilis. Meth Enzymol, 1990, 85: 199–228

Behnke D, Gerlach D. Cloning and expression in Escherichia coli, Bacillus subtilis, and Streptococcus sanguis of a gene for staphylokinase, a bacterial plasminogen activator. Mol Gen Genet, 1987, 210: 528–534

Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. New York: Cold Spring Harbor Laboratory, 1989

Chang S, Cohen S N. High-frequency transformation of Bacillus subtilis protoplast by plasmid DNA. Mol Gen Genet, 1979, 168: 111–115

Ledoux M, Lamy F. Determination of proteins and sulfobetaine with the folin-phenol reagent. Anal Biochem, 1986, 157: 28–31

Kubo M, Imanaka T. Cloning and nucleotide sequence of the highly thermostable neutral protease gene from Bacillus stearothermophilus. J Gen Microbiol, 1988, 134(7): 1883–1892

Priest F G. Extracellular enzyme synthesis in the genus Bacillus. J Bacteriol, 1977, 41: 711–753

Ayusawa D, Yoneda Y, Yamane K, et al. Pleiotropic phenomena in autolytic enzyme(s) content, flagellation, and simultaneous hyperproduction of extracellular α-amylase and protease in a Bacillus subtilis mutant. Bacteriol, 1975, 124: 459–469

Jacobs M F. Expression of the subtilisin Carlsberg-encoding gene in Bacillus licheniformis and Bacillus subtilis. Gene, 1995, 152: 69–74

Pan X F. Effects of degU32(Hy), degQa and degR pleiotropic regulatory genes on the growth and protease fermentation of Bacillus Subtilis Ki-2-132. Acta Genet Sin, 2006, 33: 373–380