Expression of the mRNA for τ Proteins During Brain Development and in Cultured Neurons and Astroglial Cells

Journal of Neurochemistry - Tập 50 Số 6 - Trang 1894-1899 - 1988
Dominique Couchie1, Cécile Charrière‐Bertrand, J. Núñez
1INSERM U.282, Hôpital Henri Mondor, Créteil France

Tóm tắt

Abstract: Two τ cDNA probes of 1.6 and 0.3 kilobases (kb) have been used to study the expression of the τ mRNAs during mouse brain development and in highly homogeneous primary cultures of neurons and astrocytes. (1) Whatever the stage, a 6‐kb mRNA was detected with the two probes. In the astrocytes a 6‐kb mRNA hybridized clearly only with the 1.6‐kb probe. (2) During brain development the abundance of τ mRNA increases from a late fetal stage (— 4 days) until birth, remains high until 6 days postnatal, and then markedly decreases to reach very low values in adulthood. Such a marked decrease in the abundance of τ mRNA parallels that of α‐tubulin mRNA. These data suggest that: (1) depending on the stage of development and on the cell type (neurons or astrocytes) τ mRNAs of the same size encode several τ proteins differing in molecular weight: several τ proteins are expressed either during early stages of development (juvenile τ proteins of 48 kilodaltons) or in adulthood (mature τ proteins of 50–70 kilodaltons) or are specific of the astrocyte (83 kilodaltons). (2) The expression of the two major components of axonal microtubules, tubulin and τ proteins, seems to be developmentally coordinated.

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Journal of Neurochemistry

Tập 50 Số 6

1894-1899

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