Biểu hiện của phân tử acid subunit của amarantin, mang các biopeptide giảm huyết áp VY, trong văn hóa tế bào treo của Nicotiana tabacum NT1

Barba de la Rosa AP, Herrera A, Utsumi S, Paredes-López O (1996) Molecular characterization, cloning and structural analysis of a cDNA encoding an amaranth globulin. J Plant Physiol 149:527–532 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 Castillo-Reyna J, Alvarez MM (2011) Effect of different agitation systems and protocols on the growth of Nicotiana tabacum cells: comparison of growth kinects in Erlenmeyer flasks, conventional stirred tanks, and eccentrically agitated tank bioreactors. Annual meeting, Minneapolis. http://apps.aiche.org/proceedings/ExtendedAbstract.aspx?PaperID=238295. Accessed 10 Feb 2012 Castro-Martínez C, Luna-Suárez S, Paredes-López O (2012) Overexpression of a modified protein from amaranth seed in Escherichia coli and effect of environmental conditions on the protein expression. J Biotechnol 158:59–67 Cruz-Mendívil A, Rivera-López J, Germán-Báez LJ, López-Meyer M, Hernández-Verdugo S, López-Valenzuela JA, Reyes-Moreno C, Valdez-Ortiz A (2011) A simple and efficient protocol for plant regeneration and genetic transformation of tomato cv. Micro-Tom from leaf explants. HortScience 46(12):1655–1660 Fido RJ, Tatham AS, Shewry PR (1995) Western blotting analysis. In: Jones H (ed) Methods in molecular biology: plant gene transfer and expression protocols. Humana Press Inc, Totowa, NJ, pp 423–437 Germán-Báez LJ (2009) Expresión de una proteína de reserva de semilla (Bioamar-KDEL) en tejido foliar, y evaluación in vitro de su actividad antihipertensiva. Dissertation, Universidad Autónoma de Sinaloa Girard LS, Bastin M, Courtois D (2004) Expression of the human milk protein sCD14 in tobacco plant cell culture. Plant Cell Tissue Organ Cult 78:253–260 Godoy-Hernández G, Vázquez-Flota FA (2006) Methods in molecular biology, Vol. 318: plant cell culture protocols. Humana press Inc., Totowa, NJ Hellwig S, Drossard J, Twiman RM, Fisher R (2004) Plant cell cultures for the production of recombinant proteins. Nat Biotech 22:1415–1422 Humiski LM, Aluko RE (2007) Physicochemical and bitterness properties of enzymatic pea protein hydrolysates. J Food Sci 72:605–611 Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol 5:387–405 Kalra S, Kalra B, Agrawal N (2010) Combination therapy in hypertension: an update. Diabetol Metab Syndr 44:2–11 Kawasaki T, Seki E, Osajima K, Yoshida M, Asada K, Matsui T, Osajima Y (2000) Antihypertensive effect of Valyl-Tyrosine, a short chain peptide derived from sardine muscle hydrolyzate, on mild hypertensive subjects. J Hum Hypertens 14(8):519–523 Kim Y, Kwon T, Sik Y (2004) Direct transfer and expression of human GM-CSF in tobacco suspension cell using Agrobacterium-mediated transfer system. Plant Cell Tissue Organ Cult 78(2):133–138 Li GH, Qu MR, Wan JZ, You JM (2007) Antihypertensive effect of rice protein hydrolysate with in vitro angiotensin 1-converting enzyme inhibitory activity in spontaneously hypertensive rats. Asia Pac J Clin Nutr 16:275–280 Luna-Suárez S, Medina-Godoy S, Cruz-Hernández A, Paredes-López O (2008) Expression and characterization of the acidic subunit from 11S amaranth seed protein. Biotech J 3:209–219 Luna-Suárez S, Medina-Godoy S, Cruz-Hernández A, Paredes-López O (2010) Modification of the amaranth 11S globulin storage protein to produce an inhibitory peptide of the angiotensin I converting enzyme, and its expression in Escherichia coli. J Biotechnol 148:240–247 Madureira AR, Tavares T, Gomes AMP, Pintado ME, Malcata FX (2010) Physiological properties of bioactive peptides obtained from whey proteins. J Dairy Sci 93:437–455 Martínez CA, Giulietti AM, Rodríguez-Talou J (2011) Expression of a KDEL-tagged dengue virus protein in cell suspension cultures of Nicotiana tabacum and Morinda citrifolia. Plant Cell Tissue Organ Cult 107:91–100 Martínez-González L, Rosales-Mendoza S, Soria-Guerra RE, Moreno-Fierros L, López-Revilla R, Korban SS, Guevara-Arauza JC, Alpuche-Solís AG (2011) Oral immunization with a lettuce-derived Escherichia coli heat-labile toxin B subunit induces neutralizing antibodies in mice. Plant Cell Tissue Organ Cult 107:441–449 Mayo KJ, Gonzales BJ, Mason HS (2006) Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens. Nat Protoc 1:1105–1111 Medina-Godoy S, Nielsen NC, Paredes-López O (2004) Expression and characterization of a His-tagged 11S seed globulin from Amaranthus hypochondriacus in Escherichia coli. Biotechnol Prog 20:1749–1756 Miguel M, Aleixandre MA, Ramos M, López-Fandiño R (2006) Effect of simulated gastrointestinal digestion on the antihypertensive properties of ACE-inhibitory peptides derived from ovalbumin. J Agric Food Chem 54:726–731 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–497 Mustafa NR, De Winter W, Van Iren F, Verpoorte R (2011) Initiation, growth and cryopreservation of plant cell suspension cultures. Nat Protoc 6:715–742 Pedroche J, Yust MM, Girón-Calle J, Alaiz M, Millán F, Vioque J (2002) Utilization of chickpea protein isolates for production of peptides with angiotensina I-converting enzyme (ACE)-inhibitory activity. J Sci Food Agric 82:960–965 Rascón-Cruz Q, Sinagawa-García S, Osuna-Castro JA, Bohorova N, Paredes-López O (2004) Accumulation, assembly, and digestibility of amarantin expressed in transgenic tropical maize. Theor Appl Genet 108:335–342 Richardson A, Fedoroff S (2001) Protocols for neural cell culture. Humana press Inc., Totowa, NJ Rosales-Mendoza S, Paz-Maldonado LMT, Govea-Alonso DO, Korban SS (2012) Engineering production of antihypertensive peptides in plants. Plant Cell Tissue Organ Cult. doi:10.1007/s11240-012-0231-9 Schinkel H, Schiermeyer A, Soeur R, Fisher R, Schillberg S (2005) Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells. Transgenic Res 14:251–259 Tovar-Pérez EG, Guerrero-Legarreta I, Farrés-González A, Soriano-Santos J (2009) Angiontensin I-converting enzyme-inhibitory peptide fractions from albumin 1 and globulin as obtained of amaranth grain. Food Chem 116:437–444 Valdez-Ortiz A, Rascón-Cruz Q, Medina-Godoy S, Sinagawa-García SR, Valverde-González ME, Paredes-López O (2005) One-step purification and structural characterization of a recombinant His-tag 11S globulin expressed in transgenic tobacco. J Biotechnol 115:413–423 Valdez-Ortiz A, Fuentes-Gutiérrez CI, Germán-Báez LJ, Gutiérrez-Dorado R, Medina-Godoy S (2012) Protein hydrolysates obtained from azufrado (Sulphur yellow) beans (Phaseolus vulgaris): nutritional, ACE-inhibitory and antioxidative characterization. LWT Food Sci Tech 46(1):91–96 Vercruysse L, Morel N, Camp JV, Szust J, Smagghe G (2008) Antihypertensive mechanism of the dipeptide Val-Tyr in rat aorta. Peptides 29:261–267 Zhang YP, Uyemoto JK, Kirkpatrick BC (1998) A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. J Virol Methods 71:45–50