Expression of soluble recombinant proteins in a cell-free system using a 96-well format

Zarembiski, 1999, Structure-based assignment of the biochemical function of a hypothetical protein: a test case for structural genomics, Proc. Natl. Acad. Sci. U. S. A., 95, 15189, 10.1073/pnas.95.26.15189

Brenner, 2001, A tour of structural genomics, Nat. Genet., 2, 801, 10.1038/35093574

Baneyx, 1999, Recombinant protein expression in Escherichia coli, Curr. Opin. Biotech., 10, 411, 10.1016/S0958-1669(99)00003-8

Spirin, 1988, A continuous cell-free translation system capable of producing polypeptides in high yield, Science, 242, 1162, 10.1126/science.3055301

Kigawa, 1991, A continuous cell-free protein synthesis system for coupled transcription-translation, J. Biochem., 110, 166, 10.1093/oxfordjournals.jbchem.a123551

Pratt, 1984, 179

Kigawa, 2002, Selenomethionine incorporation into a protein by cell-free synthesis, J. Struct. Funct. Genomics, 2, 29, 10.1023/A:1013203532303

Grodberg, 1988, OmpT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification, J. Bacteriol., 170, 1245, 10.1128/jb.170.3.1245-1253.1988

Zawadzki, 1991, Rapid and simple purification of T7 RNA polymerase, Nucleic Acids Res., 19, 1948, 10.1093/nar/19.8.1948

Monchois, 2002, Proficient target selection in structural genomics by in vitro protein expression, Biochemica, 1, 22

Knaust, 2001, Screening for soluble expression of recombinant proteins in a 96-well format, Anal. Biochem., 297, 79, 10.1006/abio.2001.5331

Rungpragayphan, 2002, High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression, J. Mol. Biol., 318, 395, 10.1016/S0022-2836(02)00094-3