Expression of microRNAs and their targets regulates floral development in tobacco (Nicotiana tabacum)
Tóm tắt
MicroRNAs (miRNAs) are an extensive class of endogenous posttranscriptional gene regulators that function to mediate gene expression by cleaving target mRNAs or by preventing protein translation. Because of their importance in mediating gene regulation, identifying and elucidating the function of miRNAs have been the primary focus of many researchers. Now that many miRNAs have been identified and assessed for their functionality, the next step is to create expression profiles for miRNAs, so that gene expression studies can be further enhanced with knowledge of the basal expression levels of miRNAs and their targets. In a previous study, 259 putative miRNAs were identified in tobacco, in which 11 of them were confirmed. The primary goal of this study was to further expand on that study and create an expression profile for nine miRNAs and their targets in a tissue-specific manner in tobacco. We chose to study miRNAs that largely play a role in floral development and nutrient stress response. The results of our study show that all tested miRNAs and their targets were expressed in a differential manner. The results of our study also show that out of the tested miRNAs and their targets, miR159, miR157, miR167, miR172, and superoxide dismutase were expressed the highest, suggesting that these genes may play a vital role in the growth and development of tobacco. Corrected expression of miRNAs and their targets regulates floral development.
Tài liệu tham khảo
Abdel-Ghany SE, Pilon M (2008) MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis. Journal of Biological Chemistry 283:15932–15945
Allen RS, Li JY, Stahle MI, Dubroue A, Gluber F, Millar AA (2007) Genetic analysis reveals functional redundancy and the major target genes of the Arabidopsis miR159 family. Proc Natl Acad Sci USA 104:16371–16376
Andrianov V, Borisjuk N, Pogrebnyak N, Brinker A, Dixon J, Spitsin S, Flynn J, Matyszczuk P, Andryszak K, Laurelli M, Golovkin M, Koprowski H (2010) Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass. Plant Biotechnol J 8:277–287
Axtell MJ, Bartel DP (2005) Antiquity of microRNAs and their targets in land plants. Plant Cell 17:1658–1673
Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136:215–233
Chiou TJ (2007) The role of microRNAs in sensing nutrient stress. Plant Cell and Environment 30:323–332
Chiou TJ, Aung K, Lin SI, Wu CC, Chiang SF, Su CL (2006) Regulation of phosphate homeostasis by microRNA in Arabidopsis. Plant Cell 18:412–421
Ding YF, Zhu C (2009) The role of microRNAs in copper and cadmium homeostasis. Biochem Biophys Res Commun 386:6–10
Doerner P (2008) Phosphate starvation signaling: a threesome controls systemic Pi homeostasis. Curr Opin Plant Biol 11:536–540
Frazier TP, Xie FL, Freistaedter A, Burklew CE, Zhang BH (2010) Identification and characterization of microRNAs and their target genes in tobacco (Nicotiana tabacum). Planta 232:1289–1308
Frazier TP, Sun GL, Burklew CE, Zhang BH (2011) Salt and drought stresses induce the aberrant expression of microRNA genes in tobacco. Mol Biotechnol 49:159–165
Frazier TP, Burklew CW, Zhang BH (2014) Titanium dioxide nanoparticles affect the growth and microRNA expression of tobacco (Nicotiana tabacum). Functional & Integrative Genomics. doi:10.1007/s10142-013-0341-4
Fujii H, Chiou TJ, Lin SI, Aung K, Zhu JK (2005) A miRNA involved in phosphate-starvation response in Arabidopsis. Curr Biol 15:2038–2043
Jones-Rhoades MW, Bartel DP, Bartel B (2006) MicroRNAs and their regulatory roles in plants. Annu Rev Plant Biol 57:19–53
Joshua SY, Donglin WC, Neal Stewart J (2008) Statistical methods for efficiency adjusted real-time PCR quantification. Biotechnol J 3:112–123
Kawashima CG, Yoshimoto N, Maruyama-Nakashita A, Tsuchiya YN, Saito K, Takahashi H, Dalmay T (2009) Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types. Plant Journal 57:313–321
Khraiwesh B, Zhu JK, Zhu JH (2012) Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants. Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms 1819:137–148
Liang G, Yang FX, Yu DQ (2010) MicroRNA395 mediates regulation of sulfate accumulation and allocation in Arabidopsis thaliana. Plant Journal 62:1046–1057
Lu XY, Huang XL (2008) Plant miRNAs and abiotic stress responses. Biochem Biophys Res Commun 368:458–462
Marschner H (1995) Mineral nutrition of higher plants. Academic Press, Inc., London
Millar AA, Gubler F (2005) The Arabidopsis GAMYB-like genes, MYB33 and MYB65, are microRNA-regulated genes that redundantly facilitate anther development. Plant Cell 17:705–721
Millar AA, Waterhouse PM (2005) Plant and animal microRNAs: similarities and differences. Functional & integrative genomics 5:129–135
Naya L, Khan GA, Sorin C, Hartmann C, Crespi M, Lelandais-Briere C (2010) Cleavage of a non-conserved target by a specific miR156 isoform in root apexes of Medicago truncatula. Plant Signaling & Behavior 5:328–331
Putterill J, Laurie R, Macknight R (2004) It’s time to flower: the genetic control of flowering time. Bioessays 26:363–373
Reyes JL, Chua NH (2007) ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination. Plant Journal 49:592–606
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF (2010) Control of cell proliferation in Arabidopsis thaliana by microRNA miR396. Development 137:103–112
USDA (2002) Census of Agriculture
USDA (2009) Census of Agriculture
Wu G, Poethig RS (2006) Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3. Development 133:3539–3547
Wu MF, Tian Q, Reed JW (2006) Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development 133:4211–4218
Wu G, Park MY, Conway SR, Wang JW, Weigel D, Poethig RS (2009) The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis. Cell 138:750–759
Xie F, Stewart CN, Taki FA, He Q, Liu H, Zhang B (2014) High-throughput deep sequencing shows that microRNAs play important roles in switchgrass responses to drought and salinity stress. Plant Biotechnology Journal, In press
Yamasaki H, Abdel-Ghany SE, Cohu CM, Kobayashi Y, Shikanai T, Pilon M (2007) Regulation of copper homeostasis by micro-RNA in Arabidopsis. J Biol Chem 282:16369–16378
Yuan JS, Reed A, Chen F, Stewart CN (2006) Statistical analysis of real-time PCR data. Bmc Bioinformatics 7:12
Zhang BH, Pan XP, Cannon CH, Cobb GP, Anderson TA (2006a) Conservation and divergence of plant microRNA genes. Plant Journal 46:243–259
Zhang BH, Pan XP, Cobb GP, Anderson TA (2006b) Plant microRNA: a small regulatory molecule with big impact. Dev Biol 289:3–16
Zhang BH, Wang QL, Pan XP (2007) MicroRNAs and their regulatory roles in animals and plants. J Cell Physiol 210:279–289