Expression of a fusion protein of Pyrus pyrifolia S-RNase with glutathione-S-transferase in E. coli
Tóm tắt
S-RNase is a style-specific ribonuclease which is associated with gametophytic self-incompatibility. An expression vector of a fusion protein of Pyrus pyrifolia(Japanese pear) S3-RNase with glutathione-S-transferase (GST) was constructed and transformed into E. coli. Using this system, the fusion protein, GST-S3-RNase, was expressed as an active form and can be used for screening pollen S-gene product(s).
Tài liệu tham khảo
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