Expression of Monovalent Fragments Derived from a Human IgM Autoantibody in E. Coli. The Input of the Somatically Mutated CDR1/CDR2 and of the CDR3 into Antigen Binding Specificity

Jahn, 1990, Strategies in the development of human monoclonal antibodies, Developm. Biol. Standard, 71, 14 Winkler, 1991, IgG human monoclonal anti-DNA autoantibodies from patients with systemic lupus erythematosus, Clin. exp. Immunol., 85, 379, 10.1111/j.1365-2249.1991.tb05735.x Grunow, 1988, The highlv efficient human B-cell immortalizing heteromyeloma CB-F7, J. Immunol. Meth., 106, 257, 10.1016/0022-1759(88)90206-2 Marks, 1991, Oligonucleotide primers for polymerase chain reaction amplification of human Ig variable genes and design of family-specific oligonucleotide probes, Eur. J. Immunol., 21, 985, 10.1002/eji.1830210419 Skerra, 1988, Assembly of a functional immunoglobulin Fv fragment in Escherzchia coli, Science, 240, 1038, 10.1126/science.3285470 Wakd, 1989, Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli, Nature, 341, 544, 10.1038/341544a0 Plückthun, 1989, Expression of functional antibody Fv and Fab fragments in Escherichia coli, Meth. Enzymol., 178, 479 Jahn, 1987, Cell biology of human IgM-producing hybridomas derived from a fusion with mouse mveloma cells, Hybridoma, 6, 679, 10.1089/hyb.1987.6.679 Shlomchik, 1990, Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation, J. exp. Med., 171, 265, 10.1084/jem.171.1.265 Berman, 1988, Content and organization of the human lgVH locus: Definition of three new VH families and linkage to the lgH locus, EMBO J., 7, 4141, 10.1002/j.1460-2075.1988.tb02869.x Jahn, 1994, Expansion of a B lymphocyte clone producing IgM auto-antibodies encoded by a somatically mutated VHI gene in the spleen of an autoimmune patient, Rheumatol. Internat., 13, 187, 10.1007/BF00390266 Hansen, 1994, VH/VL gene expression in polyreactive-antibody-producing human hybridomas from the fetal repertoire, Exp. Clin. Immungenet., 11, 1 Evans, 1985, Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product, Mol. Cell. Biol., 5, 3610, 10.1128/MCB.5.12.3610 Hochuu, 1987, New metal chelate adsorbance selective for proteins and peptides containing neighbouring histidine residues, J. Chromatogr., 411, 177, 10.1016/S0021-9673(00)93969-4 Roggenbuck, 1994, Fermentation, purification and immunochemical characterization of a natural human polyreactive monoclonal antibody, J. Immunol. Meth., 167, 207, 10.1016/0022-1759(94)90089-2 Larrick, 1989, Rapid cloning of rearranged immunoglobulin genes from human hybridoma cells by using mixed primers and polymerase chain reaction, BBRC, 160, 1250 Sambrook, 1989 Ward, 1991, Expression and secretion of T-cel receptor V alpha and V beta domains using E coli as a host, Scand. J. Immunol., 34, 215, 10.1111/j.1365-3083.1991.tb01539.x Ward, 1992, Secretion of T-cell receptor fragments from recombinant Escherichia coli cells, J. Mol. Biol., 224, 885, 10.1016/0022-2836(92)90455-S Chaudhary, 1989, Recombinant immunotoxin consisting of two antibody variable domains fused to pseudomonas endotoxin, Nature, 339, 394, 10.1038/339394a0 Sanger, 1977, DNA sequencing with chain terminating inhibitors, Proc. Natl. Acad. Sci. USA, 74, 5463, 10.1073/pnas.74.12.5463 Towbin, 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350, 10.1073/pnas.76.9.4350 Cordell, 1984, Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes), J. Histochem. Cytochem., 32, 219, 10.1177/32.2.6198355 Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Porstmann, 1985, Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase or betagalactosidase?, J. Immunol. Meth., 79, 27, 10.1016/0022-1759(85)90388-6 Friguet, 1985, Measurement of the true affinity constant in solution of antigen-antibody complexes by enzyme linked immunosorbent assay, J. Immunol. Meth., 77, 305, 10.1016/0022-1759(85)90044-4 Tron, 1989, Molecular and genetic characterization of pathogenic autoantibodies, Autoimmunity, 5, 151 Radic, 1993, Residues that mediate DNA binding of autoimmune antibodies, J. Immunol., 150, 4966 Berek, 1987, Mutation drift and repertoire shift in the maturation of the immune response, Immunol. Rev., 96, 23, 10.1111/j.1600-065X.1987.tb00507.x Hifter, 1981, Clustered arrangement of immunoglobulin lambda constant region genes in man, Nature, 294, 536, 10.1038/294536a0 Beiter, 1988, Escherichia coli secretion of an active chimeric antibody fragment, Science, 240, 1041, 10.1126/science.3285471 Skerra, 1991, Secretion and in vivo folding of the Fab fragment of the antibody MOPC603 in Escherichia coli: influence of disulfides and cis-prolines, Protein engineering, 4, 971, 10.1093/protein/4.8.971 Skerra, 1991, The functional expression of antibody Fv fragments in E, coli: improved vectors and a generally applicable purification technique. Bio/Technol., 9, 273 Roggenbuck, 1994, Real-time biospecific interaction analysis of a natural human polyreactive monoclonal IgM antibody and its Fab and scFv fragments with several antigens, Scand. J. Immunol., 40, 64, 10.1111/j.1365-3083.1994.tb03434.x Kabat, 1987