Expression of Foxa transcription factors in the developing and adult murine prostate

Prostate - Tập 62 Số 4 - Trang 339-352 - 2005
Janni Mirosevich1, Nan Gao2,1, Robert J. Matusik3,2,4,1
1Vanderbilt Prostate Cancer Center, Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, Tennessee
2Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee
3Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee
4The Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee

Tóm tắt

AbstractBackgroundThe Foxa family (a1, a2, and a3) of proteins are transcription factors that are central to endodermal development. Recently, Foxa1 has been shown to regulate the transcription of several murine and human prostate specific genes involved in differentiated function by interacting with DNA promoter sequences and androgen receptors. Currently, the developmental expression pattern of Foxa proteins in the murine prostate is unknown.MethodsMale CD‐1 mice (embryonic, prepubertal, pubertal, and adult) were used for immunohistochemical analysis of Foxa1, a2, and a3. Immunofluorescence was also performed for androgen receptor and cytokeratin 14 expression. Prostate tissue from pre‐pubertal, pubertal, and adult mice were analyzed by Western blot and RT‐PCR analysis for Foxa1, a2, and a3 expression.ResultsStrong Foxa1 immunoreactivity was observed in epithelial cells throughout prostate development, growth, and adult differentiation. Prominent Foxa2 protein expression was only observed in the early stages of prostate development and was exclusively localized to epithelial cells of the forming buds. RT‐PCR analysis identified low Foxa2 mRNA expression levels in the ventral and dorsolateral lobes of the adult prostate, with Foxa2 epithelial cell expression being localized to periurethral regions of the murine adult prostatic complex. Foxa3 expression was not observed in the murine prostate.ConclusionsFoxa proteins represent epithelial cell markers in the murine prostate gland. The early expression of Foxa1 and a2 proteins in prostate formation suggests that these proteins play an important role in normal prostate development, in addition to differentiated secretory function. © 2004 Wiley‐Liss, Inc.

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Tài liệu tham khảo

10.1016/S0012-1606(02)00031-3

10.1210/mend-2-12-1265

10.1210/edrv-8-3-338

Cunha GR, 1992, Normal and abnormal development of the male urogenital tract. Role of androgens, mesenchymal–epithelial interactions, and growth factors, J Androl, 13, 465, 10.1002/j.1939-4640.1992.tb00338.x

10.1016/S0167-4838(00)00170-9

10.1210/endo.141.12.7837

10.1210/me.7.1.23

10.1210/me.2003-0020

10.1101/gad.14.2.142

10.1006/dbio.1999.9228

10.1016/0925-4773(96)00539-4

10.1016/0092-8674(90)90439-L

10.1016/0092-8674(89)90133-5

10.1128/MCB.13.4.2401

10.1101/gad.4.8.1427

10.1242/dev.119.4.1301

10.1242/dev.118.1.47

10.1242/dev.119.3.567

10.1101/gad.13.4.495

Peterson RS, 1997, Hepatocyte nuclear factor‐3 alpha promoter regulation involves recognition by cell‐specific factors, thyroid transcription factor‐1, and autoactivation, Cell Growth Differ, 8, 69

10.1002/(SICI)1097-0177(199802)211:2<131::AID-AJA2>3.0.CO;2-I

Hogan B, 2001, Manipulating the mouse embryo, a laboratory manual

10.1677/joe.0.1620341

10.1046/j.1432-0436.2001.680414.x

Partin AW, 1998, Campbell's urology, 1381

10.1095/biolreprod34.5.961

10.1016/S0022-5347(17)35273-4

10.1095/biolreprod34.5.985

10.1159/000147793

10.1095/biolreprod45.2.308

10.1159/000147794

10.1016/S0083-6729(08)61152-8

Krongrad A, 1993, Prostate diseases, 17

10.1530/rep.0.1210187

10.1101/gad.13.8.966

10.1002/(SICI)1097-0177(199705)209:1<127::AID-AJA12>3.0.CO;2-Z

10.1128/MCB.22.5.1495-1503.2002

10.1002/1097-0177(2000)9999:9999<::AID-DVDY1054>3.0.CO;2-E

Strandberg JD, 1993, Prostate diseases, 212

10.1210/en.2002-220493

10.1002/(SICI)1097-0045(19980515)35:3<157::AID-PROS1>3.0.CO;2-E

10.1002/pros.10228

10.1096/fasebj.11.14.9409549

10.1002/(SICI)1097-0045(19980401)35:1<35::AID-PROS5>3.0.CO;2-G

10.1002/j.1939-4640.2001.tb02201.x

10.1002/(SICI)1097-0045(199601)28:1<58::AID-PROS8>3.0.CO;2-K

10.1073/pnas.96.18.10152

10.1053/jhep.2002.30317

Epstein DJ, 1999, Regionalization of Sonic hedgehog transcription along the anteroposterior axis of the mouse central nervous system is regulated by Hnf3‐dependent and‐independent mechanisms, Development, 126, 281, 10.1242/dev.126.2.281

10.1006/dbio.1999.9229

10.1242/dev.124.1.53

10.1006/dbio.1998.9103

10.1242/dev.128.21.4241

10.1006/dbio.2002.0774

10.1016/j.ydbio.2003.08.018

10.1074/jbc.M300968200

10.1002/pros.10287

10.1046/j.1464-410X.2003.03066.x

10.1111/j.1749-6632.1996.tb16227.x

Marengo SR, 2000, Advanced therapy of prostate disease, 92

10.1007/s00418-001-0335-5

10.1016/0303-7207(95)03593-V

10.1210/endo-128-6-2867

10.1002/pros.2990040406

10.1128/MCB.14.4.2755

10.1016/0012-1606(88)90260-6

Corpechot C, 1981, Testosterone, dihydrotestosterone and androstanediols in plasma, testes and prostates of rats during development, Acta Endocrinologica, 96, 127

10.1002/1097-0045(199609)29:3<137::AID-PROS2990290302>3.0.CO;2-Z

10.1002/pros.2990170405

10.1002/path.1711600409

10.1177/39.7.1865110

10.1016/0304-4165(91)90178-J

Prins GS, 1995, The developmental pattern of androgen receptor expression in rat prostate lobes is altered after neonatal exposure to estrogen, Endocrinology, 136, 1303, 10.1210/endo.136.3.7867585

10.1007/BF01605130

10.1016/S0002-9440(10)61676-8

10.1002/pros.2990150506

10.1002/(SICI)1097-0045(199602)28:2<98::AID-PROS4>3.0.CO;2-J

10.1677/erc.0.0090061

10.1016/S1043-2760(00)00271-X

10.1152/ajplung.2001.280.5.L823

10.1101/gad.13.4.495

10.1016/j.bbrc.2003.08.148

10.1126/science.281.5377.692

10.1128/MCB.16.7.3626

10.1002/(SICI)1097-0177(199711)210:3<305::AID-AJA10>3.0.CO;2-9

10.1128/MCB.20.14.5175-5183.2000

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Prostate

Tập 62 Số 4

339-352

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