Expression and regulation of β<sub>7</sub>(βp) integrins on mouse lymphocytes: Relevance to the mucosal immune system

European Journal of Immunology - Tập 21 Số 10 - Trang 2591-2597 - 1991
Peter J. Kilshaw1, Susan J. Murant2
1AFRC Institute of Animal Physiology and Genetics Research, Department of Cell Biology, Babraham, Cambridge, GB.
2AFRC Institute of Animal Physiology and Genetics Research, Department of Cell Biology, Babraham

Tóm tắt

AbstractA mouse lymphocyte surface molecule which is selectively expressed by mucosal T cells and detected with the monoclonal antibody (mAb) M290 has provisionally been identified as a β7 integrin. Identification was based on close homology of its β subunit at the N terminus with the recently reported, highly distinctive, human β7 sequence. mAb were prepared against the β and α subunits of the mouse molecule, termed β7 and αM290, respectively, and used to study surface expression of the two components. β7 was present on most lymph node lymphocytes in association with α4 rather than αM290. This integrin, β7α4, was shown to be identical to LPAM‐1 (βpα4) the Peyer's patch homing receptor. Stimulation in vitro of mouse lymph node T cells with anti‐CD3 in the presence of transforming growth factor (TGF)‐β increased β7 expression in about 40% of cells and changed the associated α chain from α4 to the novel αM290 subunit, which, in most cells, was expressed de novo. Immunoprecipitation of β7 both from these cells and from intraepithelial lymphocytes gave closely similar results and showed predominance of the β7αM290 integrin. It is suggested that in vivo this change in α‐chain usage occurs in mucosal T cells in response to TGF‐β acting in the mucosal microen‐vironment and that the new integrin confers particular adhesive properties, possibly homing specificity, on the cells.

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Tài liệu tham khảo

10.1016/0092-8674(87)90233-9

Humphries M. J., 1990, J. Cell Science, 97, 585, 10.1242/jcs.97.4.585

10.1146/annurev.iy.08.040190.002053

10.1038/346425a0

10.1002/j.1460-2075.1990.tb08170.x

10.1002/j.1460-2075.1990.tb08171.x

Ramaswarmy H., 1990, EMBO J., 9, 1561, 10.1002/j.1460-2075.1990.tb08275.x

10.1073/pnas.87.14.5354

10.1016/S0021-9258(19)38425-X

10.1093/intimm/2.11.1097

10.1016/0165-2478(88)90056-9

10.1002/eji.1830201008

10.1016/0092-8674(89)90981-1

10.1002/j.1460-2075.1989.tb03566.x

10.1136/gut.22.6.481

10.1179/his.1988.11.4.213

10.1002/eji.1830170910

Kruschwitz M. Fritzsche G. Schwarting R. Micklem K. Mason D. Y. Falini B.andStein H. J. Clin. Pathol.1991 in press.

Möller P., 1990, Am. J. Pathol, 136, 509

10.1111/j.1365-3083.1990.tb02896.x

10.4049/jimmunol.145.10.3247

10.1084/jem.173.3.599

10.1084/jem.157.3.813

10.1016/S0021-9258(17)31269-3

10.1111/j.1600-065X.1989.tb00012.x

10.1016/0092-8674(90)90155-8

10.1016/0304-419X(90)90013-Q

10.1016/0167-5699(89)90136-9

Lucas C., 1990, J. Immunol., 145, 1415, 10.4049/jimmunol.145.5.1415

10.1177/38.12.2254647

10.1111/j.1749-6632.1988.tb22355.x

10.1083/jcb.108.2.661

10.4049/jimmunol.146.3.1077

10.1084/jem.170.3.1039

10.1084/jem.173.2.471

10.1016/S0065-2776(08)60645-8

Butcher E. C., 1990, Am. J. Pathol., 136, 3

10.1111/j.1600-065X.1989.tb00010.x

10.1038/349796a0

Chin Y., 1990, J. Immunol., 145, 3669, 10.4049/jimmunol.145.11.3669

10.1016/0016-5085(88)90226-0

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European Journal of Immunology

Tập 21 Số 10

2591-2597

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