Expression and purification of recombinant human CCL5 and its biological characterization

Zhenling Ma1,2, Jiajia Zhang3, Lei Wang3, Yiying Liu3, Yunpeng Wang3, Wei Liu3, Guozhen Xing3, Kun Cheng3, Wenming Zheng3, Xiang Li4
1Henan Agricultural University
2Henan Provincial People’s Hospital/People’s Hospital of Zhengzhou University
3College of Life Sciences, Henan Agricultural University, Zhengzhou, China
4Henan Provincial People’s Hospital/People’s Hospital of Zhengzhou University, Zhengzhou, China

Tóm tắt

Từ khóa


Tài liệu tham khảo

Yi EH, Lee CS, Lee JK, Lee YJ, Shin MK, Cho CH, Kang KW, Lee JW, Han W, Noh DY, Kim YN, Cho IH, Ye SK (2013) STAT3-RANTES autocrine signaling is essential for tamoxifen resistance in human breast cancer cells. Mol Cancer Res 11(1):31–42

Conlon KC, Miljkovic MD, Waldmann TA (2019) Cytokines in the treatment of cancer. J Interferon Cytokine Res 39(1):6–21

Ouyang W, O’Garra A (2019) IL-10 family cytokines IL-10 and IL-22: from basic science to clinical translation. Immunity 50(4):871–891

Schall TJ, Bacon K, Toy KJ, Goeddel DV (1990) Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES. Nature 347(6294):669–671

Kakinuma T, Hwang ST (2006) Chemokines, chemokine receptors, and cancer metastasis. J Leukoc Biol 79(4):639–651

Luboshits G, Shina S, Kaplan O, Engelberg S, Nass D, Lifshitz-Mercer B, Chaitchik S, Keydar I, Ben-Baruch A (1999) Elevated expression of the CC chemokine regulated on activation, normal T cell expressed and secreted (RANTES) in advanced breast carcinoma. Cancer Res 59(18):4681–4687

Niwa Y, Akamatsu H, Niwa H, Sumi H, Ozaki Y, Abe A (2001) Correlation of tissue and plasma RANTES levels with disease course in patients with breast or cervical cancer. Clin Cancer Res 7(2):285–289

Aldinucci D, Borghese C, Casagrande N (2020) The CCL5/CCR5 axis in cancer progression. Cancers (Basel) 12(7):1765

Aldinucci D, Casagrande N (2018) Inhibition of the CCL5/CCR5 axis against the progression of gastric cancer. Int J Mol Sci 19(5):1477

Zheng Y, Han GW, Abagyan R, Wu B, Stevens RC, Cherezov V, Kufareva I, Handel TM (2017) Structure of CC chemokine receptor 5 with a potent chemokine antagonist reveals mechanisms of chemokine recognition and molecular mimicry by HIV. Immunity 46(6):1005–1017e5

d’Alayer J, Expert-Bezançon N, Béguin P (2007) Time- and temperature-dependent acetylation of the chemokine RANTES produced in recombinant Escherichia coli. Protein Expr Purif 55(1):9–16

Webb PM, Jordan SJ (2017) Epidemiology of epithelial ovarian cancer. Best Pract Res Clin Obstet Gynaecol 41:3–14

Jelovac D, Armstrong DK (2011) Recent progress in the diagnosis and treatment of ovarian cancer. CA Cancer J Clin 61(3):183–203

Long H, Xie R, Xiang T, Zhao Z, Lin S, Liang Z, Chen Z, Zhu B (2012) Autocrine CCL5 signaling promotes invasion and migration of CD133 + ovarian cancer stem-like cells via NF-κB-mediated MMP-9 upregulation. Stem Cells 30(10):2309–2319

Long H, Xiang T, Qi W, Huang J, Chen J, He L, Liang Z, Guo B, Li Y, Xie R, Zhu B (2015) CD133 + ovarian cancer stem-like cells promote non-stem cancer cell metastasis via CCL5 induced epithelial-mesenchymal transition. Oncotarget 6(8):5846–5859

Zhou B, Sun C, Li N, Shan W, Lu H, Guo L, Guo E, Xia M, Weng D, Meng L, Hu J, Ma D, Chen G (2016) Cisplatin-induced CCL5 secretion from CAFs promotes cisplatin-resistance in ovarian cancer via regulation of the STAT3 and PI3K/Akt signaling pathways. Int J Oncol 48(5):2087–2097

GhoshalA, Ghosh SS (2015) Expression, purification, and therapeutic implications of recombinant sFRP1. Appl Biochem Biotechnol 175(4):2087–2103

Xu T, Xiao M, Yu L (2021) Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase. Protein Expr Purif 183:105866

Liu W, Wang L, Zhang J, Qiao L, Liu Y, Yang X, Zhang J, Zheng W, Ma Z (2021) Purification of recombinant human chemokine CCL2 in E. coli and its function in ovarian cancer. 3 Biotech 11(1):8

Chen L, Cai S, Wang JM, Huai YY, Lu PH, Chu Q (2020) BRDT promotes ovarian cancer cell growth. Cell Death Dis 11(11):1021

Burotto M, Chiou VL, Lee JM, Kohn EC (2014) The MAPK pathway across different malignancies: a new perspective. Cancer 120(22):3446–3456

Labrou NE (2014) Protein purification: an overview. Methods Mol Biol 1129:3–10

Kimple ME, Brill AL, Pasker RL (2013) Overview of affinity tags for protein purification.Curr Protoc Protein Sci73: 9.9.1–9.9.23.

Jia B, Jeon CO (2016) High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives. Open Biol 6(8):160196

Wingfield PT (2015) Overview of the purification of recombinant proteins. Curr Protoc Protein Sci 80: 6.1.1–6.1.35

Smith DB, Johnson KS (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67(1):31–40

Harper S, Speicher DW (2011) Purification of proteins fused to glutathione S-transferase. Methods Mol Biol 681:259–280

Wang SW, Wu HH, Liu SC, Wang PC, Ou WC, Chou WY, Shen YS, Tang CH (2012) CCL5 and CCR5 interaction promotes cell motility in human osteosarcoma. PLoS ONE 7(4):e35101

Zazo S, González-Alonso P, Martín-Aparicio E, Chamizo C, Luque M, Sanz-Álvarez M, Mínguez P, Gómez-López G, Cristóbal I, Caramés C, García-Foncillas J, Eroles P, Lluch A, Arpí O, Rovira A, Albanell J, Madoz-Gúrpide J, Rojo F (2020) Autocrine CCL5 effect mediates trastuzumab resistance by ERK pathway activation in HER2-Positive breast cancer. Mol Cancer Ther 19(8):1696–1707

Wu YH, Huang YF, Chen CC, Huang CY, Chou CY (2020) Comparing PI3K/Akt inhibitors used in ovarian cancer treatment. Front Pharmacol 11:206

Deng Z, Sui G, Rosa PM, Zhao W (2012) Radiation-induced c-Jun activation depends on MEK1-ERK1/2 signaling pathway in microglial cells. PLoS ONE 7(5):e36739

Hsu FT, Chiang IT, Kuo YC, Hsia TC, Lin CC, Liu YC, Chung JG (2019) Amentoflavone effectively blocked the tumor progression of glioblastoma via suppression of ERK/NF-κB signaling pathway. Am J Chin Med 47(4):913–931

Li X, Bao C, Ma Z, Xu B, Ying X, Liu X, Zhang X (2018) Perfluorooctanoic acid stimulates ovarian cancer cell migration, invasion via ERK/NF-κB/MMP-2/-9 pathway. Toxicol Lett 294:44–50

Borghi A, Verstrepen L, Beyaert R (2016) TRAF2 multitasking in TNF receptor-induced signaling to NF-κB, MAP kinases and cell death. Biochem Pharmacol 116:1–10

Ge QL, Liu SH, Ai ZH, Tao MF, Ma L, Wen SY, Dai M, Liu F, Liu HS, Jiang RZ, Xue ZW, Jiang YH, Sun XH, Hu YM, Zhao YX, Chen X, Tao Y, Zhu XL, Ding WJ, Yang BQ, Liu DD, Zhang XR, Teng YC (2016) RelB/NF-κB links cell cycle transition and apoptosis to endometrioid adenocarcinoma tumorigenesis. Cell Death Dis 7(10):e2402