Expression and purification of a soluble tissue factor fusion protein with an epitope for an unusual calcium-dependent antibody

Protein Expression and Purification - Tập 3 Số 6 - Trang 453-460 - 1992
Alireza R. Rezaie1, Martine M. Fiore2, Pierre F. Neuenschwander2, Charles T. Esmon1, James H. Morrissey3
1Howard Hughes Medical Institute
2Oklahoma Medical Research Foundation
3Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA.

Tóm tắt

Từ khóa


Tài liệu tham khảo

Stearns, 1988, The interaction of a Ca2+ dependent monoclonal antibody with the protein C activation peptide region: Evidence for obligatory Ca2+ binding to both antigen and antibody, J. Biol. Chem, 263, 826, 10.1016/S0021-9258(19)35429-8

Prickett, 1989, A calcium-dependent antibody for identification and purification of recombinant proteins, Bio Techniques, 7, 580

Bach, 1988, Initiation of coagulation by tissue factor, Biochemistry, 23, 339

Paborsky, 1989, Purification of recombinant human tissue factor, Biochemistry, 28, 8072, 10.1021/bi00446a016

Ruf, 1991, Phospholipid-independent and dependent interactions required for tissue factor receptor and cofactor function, J. Biol. Chem, 266, 2158, 10.1016/S0021-9258(18)52223-7

Rehemtulla, 1991, Disulfide loop of tissue factor is required for binding of factor VII, J. Biol. Chem, 266, 10294, 10.1016/S0021-9258(18)99224-0

Lei, 1987, Characterization of the Erwinia carotovora pe1B gene and its product pectate lyase, J. Bacteriol, 139, 4379, 10.1128/jb.169.9.4379-4383.1987

Morrissey, 1987, Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade, Cell, 50, 129, 10.1016/0092-8674(87)90669-6

Ghrayeb, 1984, Secretion cloning vectors in Escherichia coli, EMBO J, 3, 2437, 10.1002/j.1460-2075.1984.tb02151.x

Morrissey, 1988, Resolution of monomeric and heteromeric forms of tissue factor, the high affinity cellular receptor for factor VII, Thromb. Res, 50, 481, 10.1016/0049-3848(88)90197-1

Morrissey, 1988, Monoclonal antibody analysis of purified and cell-associated tissue factor, Thromb. Res, 52, 247, 10.1016/0049-3848(88)90084-9

Fair, 1979, Combined functional and immunochemical analysis of normal and abnormal human factor X, J. Clin. Invest, 64, 884, 10.1172/JCI109554

Guinto, 1983

Owen, 1974, The conversion of prothrombin to thrombin. I. Characterization of the reaction products formed during the activation of bovine prothrombin, J. Biol. Chem, 249, 594, 10.1016/S0021-9258(19)43070-6

Laemmli, 1970, Cleavage of the structural proteins during assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0

Neuenschwander, 1988, A comparison of phospholipid and platelets in the activation of human factor VIII by thrombin and factor Xa, and in the activation of factor X, Blood, 72, 1761, 10.1182/blood.V72.5.1761.1761

Huse, 1989, Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda, Science, 246, 1275, 10.1126/science.2531466

Moks, 1987, Large scale affinity purification of human insulin-like growth factor I from culture medium of Escherichia coli, Biotechnology, 5, 379, 10.1038/nbt0487-379

Nagahari, 1985, Secretion into the culture medium of a foreign gene product from Escherichia coli. Use of the ompF gene for secretion of human endorphin, EMBO J, 4, 3589, 10.1002/j.1460-2075.1985.tb04121.x

Le, 1991, Purification and analysis of recombinant proteins, 163