Expression and purification of a soluble form of penicillin-binding protein 2 from both penicillin-susceptible and penicillin-resistant Neisseria gonorrhoeae

Protein Expression and Purification - Tập 2 - Trang 339-349 - 1991
Derk E. Schultz1, Brian G. Spratt2, Robert A. Nicholas1
1Department of Pharmacology, CB 7365 FLOB, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7365, USA
2Microbial Genetics Group, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom

Tài liệu tham khảo

Spratt, 1975, Distinct penicillin-binding proteins involved in the division, elongation, and shape of Escherichia coli, 72, 2999 Spratt, 1983, Penicillin-binding proteins and the future of beta-lactam antibiotics. The Seventh Fleming Lecture, J. Gen. Microbiol, 129, 1247 Waxman, 1983, Penicillin-binding proteins and the mechanism of action of β-lactam antibiotics, Annu. Rev. Biochem, 52, 825, 10.1146/annurev.bi.52.070183.004141 Ishino, 1980, Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin-sensitive transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A, Biochem. Biophys. Res. Commun, 97, 287, 10.1016/S0006-291X(80)80166-5 Ishino, 1981, Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: A septum-forming sequence, Biochem. Biophys. Res. Commun, 101, 905, 10.1016/0006-291X(81)91835-0 Ishino, 1986, Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and RodA protein, J. Biol. Chem, 261, 7024, 10.1016/S0021-9258(19)62717-1 Spratt, 1988, Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae, Nature, 332, 173, 10.1038/332173a0 Barbour, 1981, Properties of penicillin-binding proteins in Neisseria gonorrhoeae, Antimicrob. Agents Chemother, 19, 316, 10.1128/AAC.19.2.316 Dougherty, 1980, Penicillin-binding proteins of penicillin-susceptible and intrinsically resistant Neisseria gonorrhoeae, Antimicrob. Agents Chemother, 18, 730, 10.1128/AAC.18.5.730 Dougherty, 1986, Genetic analysis and penicillin-binding protein alterations in Neisseria gonorrhoeae with chromosomally mediated resistance, Antimicrob. Agents Chemother, 30, 649, 10.1128/AAC.30.5.649 Cannon, 1984, The genetics of the gonococcus, Annu. Rev. Genet, 38, 111 Faruki, 1986, Genetics of resistance in a non-β-lactamase-producing gonococcus with relatively high-level penicillin resistance, Annimicrob. Agents Chemother, 30, 856, 10.1128/AAC.30.6.856 Brannigan, 1990, Insertion of an extra amino acid is the main cause of the low affinity of penicillin-binding protein 2 in penicillin-resistant strains of Neisseria gonorrhoeae, Mol. Microbiol, 4, 913, 10.1111/j.1365-2958.1990.tb00664.x Dowson, 1989, Penicillin-binding protein 2 genes of non-beta-lactamaseproducing, penicillin-resistant strains of Neisseria gonorrhoeae, Mol. Microbiol, 3, 35, 10.1111/j.1365-2958.1989.tb00101.x Ishino, 1988, Crystallization and preliminary crystallographic studies of the high molecular weight penicillin-binding protein PBP 1B-γ of Escherichia coli, 285 Pratt, 1986, The C terminus of penicillin-binding protein 5 is essential for localisation to the E. coli inner membrane, EMBO J, 5, 2399, 10.1002/j.1460-2075.1986.tb04510.x Ferriera, 1990, Properties and crystallization of a genetically engineered, water soluble derivative of penicillin binding protein 5 of Escherichia coli, Eur. J. Biochem, 171, 11, 10.1111/j.1432-1033.1988.tb13751.x Nicholas, 1990, Site-directed mutants of a soluble form of penicillin-binding protein 5 from Escherichia coli and their catalytic properties, J. Biol. Chem, 263, 2034, 10.1016/S0021-9258(19)77981-2 Adachi, 1987, A water-soluble form of penicillin-binding protein 2 of Escherichia coli constructed by site directed mutagenesis, FEBS Lett, 226, 150, 10.1016/0014-5793(87)80569-0 Bowler, 1989, Membrane topology of penicillin-binding protein 3 of Escherichia coli, Mol. Microbiol, 3, 1277, 10.1111/j.1365-2958.1989.tb00278.x Bartholomé-De Belder, 1988, Overexpression, solubilization and refolding of a genetically engineered derivative of the penicillin-binding protein 3 of Escherichia coli K12, Mol. Microbiol, 2, 519, 10.1111/j.1365-2958.1988.tb00058.x Spratt, 1986, Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9, Gene, 41, 337, 10.1016/0378-1119(86)90117-4 Kunkel, 1987, Rapid and efficient site-specific mutagenesis without phenotypic selection, 154, 367 Sanger, 1977, DNA sequencing with chain terminating inhibitors, 74, 5463 Marston, 1990, Purification of calf prochymosin (prorenin) synthesized in Escherichia coli, Bio/technology, 2, 800, 10.1038/nbt0984-800 Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Morrissey, 1981, Silver strain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity, Anal. Biochem, 117, 307, 10.1016/0003-2697(81)90783-1 Nakamura, 1983, On the process of cellular division in Escherichia coli: Nucleotide sequence of the gene for penicillin-binding protein 3, Mol. Gen. Genet, 191, 1, 10.1007/BF00330881 Hara, 1989, Genetic analyses of processing involving C-terminal cleavage in penicillin-binding protein 3 of Escherichia coli, J. Bacteriol, 171, 5882, 10.1128/jb.171.11.5882-5889.1989 Nagasawa, 1989, Determination of the cleavage site involved in C-terminal processing of penicillin-binding protein 3 of Escherichia coli, J. Bacteriol, 171, 5890, 10.1128/jb.171.11.5890-5893.1989 Hayashi, 1988, Lipid modification of Escherichia coli penicillin binding protein 3, J. Bacteriol, 170, 5392, 10.1128/jb.170.11.5392-5395.1988 Tabor, 1985, A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes, 82, 1074 Marston, 1986, The purification of eukaryotic polypeptides synthesized in Escherichia coli, Biochem. J, 240, 1, 10.1042/bj2400001 Ghuysen, 1986, Active-site- serine d-alanyl-d-alanine-cleaving-peptidase-catalysed acyl-transfer reactions, Biochem. J, 235, 159, 10.1042/bj2350159 Hartman, 1984, Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus, J. Bacteriol, 158, 513, 10.1128/JB.158.2.513-516.1984 Dowson, 1990, Extensive remodelling of the transpeptidase domain of penicillin-binding protein 2B of a penicillin-resistant South African isolate of Streptococcus pneumoniae, Mol. Microbiol, 3, 95, 10.1111/j.1365-2958.1989.tb00108.x Dowson, 1989, Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae, 86, 8842 Smith, 1991, Localized sex in bacteria, Nature, 349, 29, 10.1038/349029a0 Hedge, 1985, Amino acid substitutions that reduce the affinity of penicillin-binding protein 3 of Escherichia coli for cephalexin, Eur. J. Biochem, 151, 111, 10.1111/j.1432-1033.1985.tb09075.x Hedge, 1985, Resistance to beta-lactam antibiotics by re-modelling the active site of an E. coli penicillinbinding protein, Nature, 318, 478, 10.1038/318478a0