Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation

Protein Expression and Purification - Tập 82 Số 1 - Trang 162-167 - 2012
Yifeng Li1, Rui Sousa1
1Protein Production Core Facility, Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.

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Tài liệu tham khảo

Weber, 1989, Structural origins of high-affinity biotin binding to streptavidin, Science, 243, 85, 10.1126/science.2911722

Bayer, 1990, Protein biotinylation, Methods Enzymol., 184, 138, 10.1016/0076-6879(90)84268-L

Barker, 1981, The birA gene of Escherichia coli encodes a biotin holoenzyme synthetase, J. Mol. Biol., 146, 451, 10.1016/0022-2836(81)90042-5

Schatz, 1993, Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli, Biotechnology (NY), 11, 1138, 10.1038/nbt1093-1138

Beckett, 1999, A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation, Protein Sci., 8, 921, 10.1110/ps.8.4.921

Cull, 2000, Biotinylation of proteins in vivo and in vitro using small peptide tags, Methods Enzymol., 326, 430, 10.1016/S0076-6879(00)26068-0

Crawford, 1998, Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes, Immunity, 8, 675, 10.1016/S1074-7613(00)80572-5

Duffy, 1998, Site-specific, enzymatic biotinylation of recombinant proteins in Spodoptera frugiperda cells using biotin acceptor peptides, Anal Biochem., 262, 122, 10.1006/abio.1998.2770

Chen, 2005, Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase, Nat. Methods., 2, 99, 10.1038/nmeth735

Howarth, 2008, Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin, Nat. Protoc., 3, 534, 10.1038/nprot.2008.20

Tropea, 2009, Expression and purification of soluble His(6)-tagged TEV protease, Methods Mol. Biol., 498, 297, 10.1007/978-1-59745-196-3_19

Howarth, 2005, Targeting quantum dots to surface proteins in living cells with biotin ligase, Proc. Natl. Acad. Sci. USA, 102, 7583, 10.1073/pnas.0503125102