Expression Vector pLF22 for Lactic Acid Bacteria
Tóm tắt
The construction of the expression vector pLF22 for lactic acid bacteria is described. The vector contains a replicon of the cryptic plasmid pLF1311 from Lactobacillus fermentum and a multiple cloning site of the lacZ′ gene integrated with the plasmid rep operon. Such a construction of the vector provides for the constitutive transcription of the cloned sequences lacking the terminators of transcription in all the strains that maintain the replication of the vector. The vector is suitable for a wide range of gram-positive and gram-negative bacteria, including probiotic strains. The efficiency of the vector was verified by expressing the β-galactosidase gene in a laboratory Escherichia coli strain and the synthetic gene of growth hormone-releasing factor (GRF) in the probiotic strains of lactobacilli and enterococci. A recombinant strain with the GRF gene included in the diet of laboratory animals exerted affected characteristic features of their physiology, anatomy, and growth.
Tài liệu tham khảo
Pouwels, P.H., Leer, R.J., and Boersma, W.J.A., The Potential of Lactobacillus as a Carrier for Oral Immunization: Development and Preliminary Characterization of Vector Systems for Targeted Delivery of Antigens, J. Biotechnol., 1996, vol. 44, pp. 183–192.
Djordjevic, G.M. and Klaenhammer, T.R., Positive Selection, Cloning Vectors for Gram-Positive Bacteria Based on a Restriction Endonuclease Cassette, Plasmid, 1996, vol. 35, pp. 37–45.
Aleshin, V.V., Semenova, E.V., Tarakanov, B.V., and Livshits, V.A., A Family of Shuttle Vectors for Lactic Acid Bacteria and Other Gram-Positive Bacteria Based on the Plasmid pLF1311 Replicon, Mikrobiologiya, 2000, vol. 69, pp. 75–80.
McCracken, A., Turner, M.S., Giffard, P., Hafner, L.M., and Timms, P., Analysis of Promoter Sequences from Lactobacillus and Lactococcus and Their Activity in Several Lactobacillus Species, Arch. Microbiol., 2000, vol. 173, pp. 383–389.
Luoma, S., Peltoniemi, K., Joutsjoki, V., Rantanen, T., Tamminen, M., Heikkinen, I., and Palva, A., Expression of Six Peptidases from Lactobacillus helveticus in Lactococcus lactis, Appl. Environ. Microbiol., 2001, vol. 67, pp. 1232–1238.
Pavlova, S.I., Kilic, A.O., Topisirovic, L., Miladinov, N., Hatzos, C., and Tao, L., Characterization of a Cryptic Plasmid from Lactobacillus fermentum KC5b and Its Use for Constructing a Stable Lactobacillus Cloning Vector, Plasmid, 2002, vol. 47, pp. 182–192.
Aleshin, V.V., Doroshenko, V.G., and Tarakanov, B.V., The 3'-Terminus of the repB gene of the Lactobacillar Plasmid pLF1311 Increased Due to the Loss of the Stop Codon of the Precursor Gene, Mol. Biol. (Moscow), 1998, vol. 32, pp. 416–419.
Yakovleva, A.A., Aleshin, V.V., and Tarakanov, B.V., The Structural Instability of Some Artificial Plasmids and Their Heterologous Expression from the Native Promoters, Dokl. Ross. Akad. Selsk. Nauk, 2001, no. 2, pp. 33–36.
Brenig, B. and Brem, G., Comparative Analysis of Transgenic Expression in Mice, Chim. OGGI, 1993, vol. 11,no. (1–2), pp. 33–42.
Miller, J.H., Experiments in Molecular Genetics, Cold Spring Harbor: Cold Spring Harbor Lab., 1972. Translated under the title Eksperimenty v molekulyarnoi genetike, Moscow: Mir, 1976.
Manukhina, A.I., Tarakanov, B.V., Nikolicheva, T.A., and Bruskova, O.B., The Effect of Some Probiotics on the Structural Organization of the Rabbit Immune System Organs, Trudy VNIIFBP, 2001, vol. 40, pp. 104–111.
Espinosa, M., del Solar, G., Rojo, F., and Alonso, J.C., Plasmid Rolling Circle Replication and Its Control, FEMS Microbiol. Lett., 1995, vol. 130, pp. 111–120.
Sassolas, G., Growth Hormone-Releasing Hormone: Past and Present, Horm. Res., 2000, vol. 53,suppl. 3, pp. 88–92.
Sainz, R.D., Hosking, B.J., Hart, F.J., and Schricker, B.R., Exogenous Growth Hormone-Releasing Factors and Cottonseed Meal Improve Growth Performance and Composition of Gain in Lambs Fed Lucerne Chaff Ad Libitum, Austr. J. Agric. Res., 1994, vol. 45, pp. 1111–1123.
Ryabykh, V.P., Kal'nitskii, B.D., and Ernst, L.K., On the Use of the Somatotropic Cascade Genes for Obtaining Transgenic Pigs with a Specified Productivity and Quality, Gennoinzh. Selsk. Zhivotn., 1995, no. 1, pp. 289–325.
Draghia-Akli, R., Ellis, K.M., Hill, L.A., Malone, P.B., and Fiorotto, M.L., High-Efficiency Growth Hormone-Releasing Hormone Plasmid Vector Administration into Skeletal Muscle Mediated by Electroporation in Pigs, FASEB J., 2003, vol. 17, pp. 526–528.
Ziv, E. and Bendayan, M., Intestinal Absorption of Peptides through the Enterocytes, Microsc. Res. Techniq., 2000, vol. 49,no. 4, pp. 346–352.
Philipps, A.F., Kling, P.J., Grille, J.G., and Dvorak, B., Intestinal Transport of Insulin-Like Growth Factor-I (IGF-I) in the Suckling Rat, J. Pediatr. Gastroenterol. Nutr., 2002, vol. 35,no. 4, pp. 539–544.
Schally, A.V., Arimura, A., Bowers, C.Y., Kastin, A.J., Sawano, S., and Reeding, T.W., Hypothalamic Neurohormones Regulating Anterior Pituitary Function, Rec. Prog. Horm. Res., 1968, vol. 24, pp. 497–588.
Garcia-Fernandez, M.O., Schally, A.V., Varga, J.L., Groot, K., and Busto, R., The Expression of Growth Hormone-Releasing Hormone (GHRH) and Its Receptor Splice Variants in Human Breast Cancer Lines: The Evaluation of Signaling Mechanisms in the Stimulation of Cell Proliferation, Breast Cancer Res. Treat., 2003, vol. 77, pp. 15–26.