Experimental infection of pigs with aTaenia species from Korea: parasitological and serological aspects

Parasitology Research - Tập 78 - Trang 513-515 - 1992
S. Geerts1, A. Zorloni1, V. Kumar1, J. R. A. Brandt1, R. de Deken1, K. S. Eom2
1Institute of Tropical Medicine, Antwerp 1, Belgium
2Department of Parasitology, College of Medicine, Chungbuk National University, Chungbuk, Republic of Korea

Tóm tắt

Belgian Landrace piglets were experimentally infected with eggs of aTaenia sp. of Korean origin. At autopsy, metacestodes were present only in the livers. The proportion of degenerated metacestodes increased from 12%–39% at 5 weeks to 94%–100% at 10 weeks after infection. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the excretory-secretory products ofT. saginata metacestodes detected circulating antigen in the sera of the pigs at 1 week post-infection. A good correlation was found between the presence of viable metacestodes and the detection of circulating antigen; the latter disappeared as the metacestodes died off. However, the antibodies were detected only after 3 weeks of infection and onwards until the necropsy of the pigs.

Tài liệu tham khảo

Brandt JRA, Geerts S, De Deken R, Kumar V, Ceulemans F, Brijs L, Falla N (1992) A monoclonal antibody based ELISA for the detection of circulating excretory-secretory antigens inTaenia saginata cysticercosis. Int J Parasitol (in press) Eom KS, Rim HJ (1988) Pig as possible intermediate host ofTaenia saginata. Korean J Parasitol 26:304 Fan PC (1988) TaiwanTaenia and taeniasis. Parasitol Today 4:86–88 Fan PC, Lin CY, Kosman ML, Kosin E (1989a) Experimental infection of IndonesiaTaenia (Samosir strain) in domestic animals. Int J Parasitol 19:809–812 Fan PC, Lin CY, Wu CC, Chung WC, Soh CT (1989b) Experimental studies of KoreaTaenia (Cheju strain) infection in domestic animals. Ann Trop Med Parasitol 83:395–403 Fan PC, Chung WC, Lin CY, Wu CC (1990a) Experimental infection of ThailandTaenia (Chiengmai strain) in donestic animals. Int J Parasitol 20:121–123 Fan PC, Chung WC, Lin CY, Wu CC (1990b) The pig as an intermediate host for TaiwanTaenia infection. J Helminthol 64:223–231 Fan PC, Chung WC, Lo CT, Lin CY (1990c) The pig as an experimental host ofTaenia sagineta (Ethiopia and Madagascar strains). Ann Trop Med Parasitol 84:93–95 Rhoads ML, Murrell KD, Cross JH, Fan PC (1989) The serological response of pigs experimentally infected with a species ofTaenia from Taiwan. Vet Parasitol 30:279–285 Sokal RS, Rohlf JJ (1981) Biometry. The principles of statisties in biological research, 2nd edn. Freeman & Cie. New York Zarlenga DS, McManus DP, Fan PC, Cross JH (1991) Characterization and detection of a newly described Asiar taeniid using cloned ribosomal DNA fragments and sequence amplification by the polymerase chain reaction. Exp Parasitiol 72:174–183