Experimental cutaneous wound healing in rabbits: using continuous microamperage low-voltage electrical stimulation
Tóm tắt
This study was designed to determine the effects of continuous microamperage low-voltage electrical stimulation on cutaneous wound healing. Sixty mature rabbits were randomly divided into three equal groups (experimental, open control, and closed or sutured control groups). After routine surgical preparations, two 3 × 1 cm pieces of lumbosacral skin were excised on both sides in each animal. An incision was made over the fascia and muscle on the right side (deep wounds), and in the left side, only the skin was removed (superficial wound). Continuous direct electrical current (100 μA and 1.5 V) was applied to both wounds of the experimental group for 14 days. All rabbits were kept under observation for a period of 21 days, and their wound contraction and repair were measured daily. The rabbits then were euthanized, and biopsies were taken from the site of initial incisions. There was no significant difference in the rate of wound contraction between experimental group and open control. The yield and ultimate strength of the above mentioned specimens were lower than those of the normal skin, and the differences in biomechanical parameters between all groups were not statistically significant. There was a statistically significant decrease in the biomechanical properties of closed control lesions compared to those of the open control (p < 0.05). Hemorrhages were evident in the upper dermis just below the epidermis, and many macrophages and lymphocytes were infiltrated at the site of injury. Electron microscopic studies showed no significant difference in the collagen fibrils diameter and distribution between different groups. There was no significant difference in the percentage dry weight of the injured skin with those of the normal skin. Results suggest that continuous microamperage low-voltage electrical stimulation, as given, did not significantly improve wound healing.
Tài liệu tham khảo
Alvarez OM, Mertz PM, Smerbeck RV et al (1983) The healing of superficial skin wounds is stimulated by external electrical current. J Invest Dermatol 81:144–148
Assimacopoulos D (1968) Wound healing promotion by the use of negative electric current. Am Surg 34:423–431
Bayat M, Asgari- Moghadam Z, Maroufi M et al (2006) Experimental wound healing using microamperage stimulation in rabbits. J Rehabil Res Dev 43:219–226
Bourgiugnon GJ, Bourgiugnon LY (1987) Electric stimulation of protein and DNA synthesis in human fibroblasts. FASEB J 1:398–402
Brown M, Gogia PP (1987) Effects of high voltage stimulation on Cutaneous wound healingin rabbits. Phys Ther 67:662–667
Brown M, McDonnel MK, Menton DN (1988) Electrical stimulation effect on cutaneous wound healing in rabbits. Phys Ther 68:655–660
Brown M, McDonnel MK, Menton DN (1989) Polarity effects on wound healing using electric stimulation in rabbits. Arch Phys Med Rehabil 70:624–627
Butler DL, Grood ES, Noyes FR (1978) Biomechanics of ligaments and tendons. Exerc Sport Sc Rev 6:125–181
Byl NN, Mckenzie AL, West JM et al (1994) Pulsed microamperage stimulation: a controlled study of healing of surgically induced wounds in Yucatan pigs. Phys Ther 74:201–219 Erratum in: Phys Ther 1994; 74: 595)
Canseven AG, Atalay NS (1996) It is possible to trigger collagen synthesis by electric current in skin wounds. Indian J Biochem Biophys 33:223–227
Carley PJ, Wainapel SF (1985) Electrotherapy for acceleration of wound healing: low intensity direct current. Arch Phys Med Rehabil 66:443–446
Demir H, Balay H, Kirnap M (2004) A comparative study of the effects of electrical stimulation and laser treatment on experimental wound healing in rats. J Rehabil Res Dev 41:147–154
Friedenberg ZB, Andrews T, Smolenski BI et al (1970) Bone reaction to varying amounts of direct current. Surg Gynecol Obstet 131:894–899
Gault WR, Gatens PF (1976) Use of low intensity direct current in management of ischemic skin ulcers. Phys Ther 56:265–269
Gentzkow GD (1993) Electrical stimulation to heal dermal wounds. J Dermatol Surg Oncol 19:753–758
Goh JC, Bose K, Kang YK et al (1988) Effects of electrical stimulation on the biomechanical properties of facture healing in rabbits. Clin Orthop Relat Res 233:268–273
Griffin JW, Tooms RE, Mendius RA et al (1991) Efficacy of high voltage pulsed current for healing of pressure ulcers in patients with spinal cord injury. Phys Ther 71:433–442
Hooker DN (1994) Electrical stimulation currents. In: Prentice WE (ed) Therapeutic modalities in sport medicine. William, Brown Co., St. Louis, pp 51–88
Karnovsky MJ (1965) A formaldehyde-glutaralehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137
Leffman DJ, Arnall DA, Holmgren PR et al (1994) Effect of microamperage stimulation on the rate of wound healing in rats: a historical study. Phys Ther 74:195–200
Nessler JP, Mass DP (1987) Direct-current electrical stimulation of tendon healing in vitro. Clin Orthop Relat Res 217:303–312
Oryan A, Zaker SR (1988) Effects of topical application of honey on cutaneous wound healing in rabbits. J Vet Med A 45:181–188
Oryan A, Meimandi Parizi AH, Dehghani S (1996) Biomechanical and ultrastructural studies on effects of electrical stimulation on experimental fracture healing in rabbit. Indian J Vet Surg 17:98–105
Peacock EE, Cohen IK (1990) Wound healing. In: McCarthy JG, May JW, Littler JW (eds) Plastic surgery. Saunders, Philadelphia, pp 161–185
Reger SI, Hyodo A, Negami S et al (1999) Experimental wound healing with electrical stimulation. Artif Organs 23:460–462
Sharrard WJ (1990) A double-blind trial of pulsed electromagnetic fields for delayed union of tibial fractures. J Bone Joint Surg Br 72:347–355
Taskan I, Ozyazgan I, Tercan M et al (1997) A comparative study of the effects of ultrasound and electric stimulation on wound healing in rats. Plastic Recnstr Surg 100:966–972
Watson T (1994) Electrical stimulation for wound healing. In: Bazin S, Kitchen S (eds) Clayton’s electrotherapy. Saunders, Philadelphia, pp 223–397
Woo SLY, Ritter MA, Amiel D et al (1980) The biomechanical and biochemical properties of swine tendon- long term effects of exercise on the digital extensors. Conn Tissue Res 7:177–183
Wu KT, Go N, Dennis C et al (1967) Effects of electric currents and interfacial potentials on wound healing. J Surg Res 7:122–128
Yarkony GM (1994) Pressure ulcers: a review. Arch Phys Med Rehabil 75:908–917